Expression Pattern of Human ADAMTS-2 (A disintegrin and metalloproteinase with thrombospondin motif, 2) in Different Cell Lines

Abstract

The ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs) are a family of metalloproteases that are found in mammals and invertebrates. This family is known to influence development, angiogenesis, coagulation and progression of arthritis. ADAMTS-2 plays an essential role in the processing of the fibrillar collagen precursors into mature collagen molecules by cleaving of the amino terminus of the type I, II, III and V procollagen molecules. Collagens are the most abundant and important components of the extracellular matrix. Mutations in the ADAMTS-2 gene cause Ehler Danlos Syndrome Type VIIC in humans. Expression analysis of the ADAMTS-2 in some human tissues have been investigated so far. There is restricted studies on the expression pattern of ADAMTS-2 gene in cell lines. In this study, ADAMTS-2 mRNA expression levels were determined by semiquantitative RT-PCR analysis in MG-63, Saos-2, DU-145, PC-3, HUVEC, HeLa, HL-60 and Isichawa cell lines. ADAMTS-2 protein was visualised in Saos-2 osteosarcoma cell model by immunofluorescence for the first time. This preliminary studies about ADAMTS-2 gene expression levels will be beneficial for further ADAMTS-2 gene regulation studies in different cancer models.