Investigation on Cryptosporidium spp. in water samples collected from River Melet in Ordu by Loop Mediated Isothermal Amplification (LAMP)

  • Zeynep KOLOREN
  • Elif DEMİREL
  • Elif DEMİREL
Keywords: Cryptosporidium, LAMP, River Melet


Cryptosporidium spp. were identified in water samples collected from River Melet in Ordu Province by Loop Mediated Isothermal Amplification (LAMP). The water samples are regularly taken every month from River Melet where mix the sea point (M-1), near solid waste storage area (M-2), Ordu-Giresun on the highway bridge location (M-3), near the city's industry (M-4) and in the city out (M-5) from December, 2010 to November, 2011. 60 water samples were flocculated by Aluminum Sulfate and they were used for the counting of Cryptosporidium sp. oocysts by Modified Acid-Fast (MAF). The highest number of samples were purified by sucrose-gradient and DNA were extracted for LAMP assay. The number of oocycsts decreased in the form of M-3, M-4, M-2, M-1 ve M-5, respectively in five stations of River Melet. The six water samples from per site, which is the highest numbers of oocysts by MAF, were analyzed by LAMP and they were found positive. Ordu has rich water resources, but, the availability of them have been reduced because of the waste waters discharge of river and sea directly without processing. Parasites move to rivers, lakes and streams by rainfall in this area. Thereby, the drinking, agriculture and recreational use of water is important to identify potential risk factors for the protection of the public health.