Isolation and Characterisatıon of Acidic Cellulase Enzyme from Native Bacillus Sp. S-41 Strain

  • Sureyya KOCAMAZ
  • Derya KOCAMAZ
Keywords: Acidic cellulase, Bacillus, Thermostable, pH stable


Acidic cellulase producing Bacillus sp. S-41was screened from soil samples in Ardicli village, Tarsus, Turkey and was optimized for the production of extracellular cellulolytic enzyme (CMCase). Using SDS-PAGE one band is found with molecular mass 53.7 kDa. The partially purified enzyme showed optimally activity at pH 4.0 (average 88%) and 50°C and it was stable from pH 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0 after incubating at 37 °C for 24 hour 82%, 96%, 94%, 92%, 90%, 81% and 70%, respectively. Enzyme retained its original activity at 50, 60 and 100°C for 60 min 100%, 93%, 70%, respectively. While the enzyme activity was increased in the presence of (1% v/v) β-merkaptoethanol 145% , EDTA (5mM) 64%, CaCl2 (5mM) 58%, ZnCl2 (5mM) 59%, Na-sulfite (5 mM) 60%, 1,10-phenantroline (5mM) 53%, MnCl2 PMSF (3mM) 55%, TritonX100 (1% v/v) 61%, SDS (1% v/v) 60% and Urea (8M) 62% were inhibited respectively. According to these results, Bacillus sp. S-41 cellulase enzyme showed acid active and stable, meso-thermophile (between at 40-90 °C, 88% active), highly thermostable and metallo-enzyme properties. Therefore it has the potentials to use in cellulose, beverage, prebiotic, feed and food industries areas, bioremediation applications and second generation bioethanol production.