Thidiazuron (TDZ) Induced in Vitro Axillary Shoot Regeneration of Desi Chikpea (Cicer arietinum L.)

  • Arife Kirtiş
  • Muhammad Aasim1
Keywords: Desi chickpea, in vitro, Shoot tip, Cotyledonary node


Desi Chcikpea (Cicer auritinum L.) is samll sized chickpea of blackish color with rough seed coat. Desi Chcikpea is very popular in Indian subcontinent due to its use as in making food and also as medicinal plant especially against Diabetes. Desi chickpea is also cultivated
crop in SouthEastern Anatolian region of Turkey. shoot tip and cotyledonary node explants excised from in vitro germinated seeds were inoculated on MS medium fortified with different TDZ concentrations (0.25-3.0 mg/L). Multiple shoot formation without any callusing was evidented after four weeks of culture. Both explants and interactive effect of explants X TDZ concentration was insignificant. Whereas, TDZ concentrations yielded 93.33-100.00% shoot formation frequency. Contrarily, explants, TDZ concentration and interactive effect of explants X TDZ concentration was statistically significant. Shoot tip explant yielded 12.54 shoots compared to 11.46 shoots for cotyledonary node. The
highest shoot counts of 17.90 were recorded for medium augmented with 0.25 mg/L TDZ. Similarly, maximum shoot counts from explants X TDZ concentration were also recorded on medium augmented with 0.25 mg/L TDZ for both explants. In vitro regenerated shoots were shorter in length and failed to root on the rooting medium enrched with 0.25-1.00 mg/l IBA. The results revealed that both explants can be used for multiple shoot formation of desi chickpea. However, it is recommended to use other explants and plant growth regulators to obatin normal shoots for rooting and acclimatization.