ACUTE TOXIC EFFECT OF ETHYLENEDIAMINE DIHYDROCHLORIDE (EDA-2HCl) ON LIVER AND KIDNEY
Ethylenediamine dihydrochloride (EDA-2HCl, 50 mg/kg/day) was given to Wistar albino rats for ten days and the histopathologically effects on liver and kidney were examined by electron microscope. Besides the effects of EDA-2HCl on superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) activities and amount of total sialic acid (TSA) and malondialdehyde (MDA) in plasma and tissues, amount of total protein in tissues were determined. Degeneration of membrane in hepatocytes and in tubule cell in kidney; dysmorphism, loosing of Krista and increasing of count of mitochondria in hepatocytes and hypertrophy in kidney; reduction of intensity of nuclear matrix, loosing of cytoplasm, swelling of smooth endoplasmic reticulum (SER) in hepatocyte and tubule cell; dilatation and decreasing of microvillus of bile canaliculi in hepatocytes, untidiness of basal membrane and derangement of basal infoldings in kidney were observed. Amount of TSA was increased significantly (p<0.05) only in plasma and decreased significantly (p<0.001) only in kidney. Amount of MDA was increased in plasma, liver and kidney significantly (p<0.001). SOD activity was decreased significantly (p<0.001) only in liver. GPX and CAT activities were not changed in liver and kidney. Amount of total protein was decreased only in kidney significantly (p<0.001). These findings indicate that EDA-HCl has toxic effect on ultrastructural morphology and also on these biochemical parameters even in this lowest dose untested before. Therefore we believe that the toxic potential of EDA-HCl should be investigated further by using lower doses in details.