ROLE OF ISSR MOLECULAR MARKER IN ANALYSIS OF GENETIC DIVERSITY OF MUTANT Glycine soja THROUGH MUTATION INDUCTION WITH COMBINATION OF GAMMA RAY IRRADIATION AND EMS (ETHYL METHANE SULFONATE)

Abstract

Mutation induction in soybeans is performed to achieve a high degree of genetic diversity as a basis for plant breeding in an attempt to obtain desired varieties. It is performed physically using gamma rays and chemically using EMS mutagen. This research was intended to figure out the effectiveness of the combination of chemical (EMS) and physical mutagens (gamma rays) in the induction of genetic diversity in black soybeans. Mutation detection can be carried out with molecular markers to characterize the genetic diversity of a crop. The mutation induction method employed in this research involved EMS and gamma-ray treatments. This research made use of 5-primer ISSR molecular marker. The UBC 810, UBC 811, UBC 812, UBC 828, and K18 primers used had successfully amplified and demonstrated genetic diversity in the black soybeans which were subjected to gamma-ray irradiation and EMS administration. The study results indicate that gamma-ray irradiation treatment yielded a higher extent of polymorphism than did control and EMS treatment, thus fit to be applied to candidate parent plants for further treatments.