VITAMIN-D3 INDUCED CHANGES IN THE CORPUSCLES OF STANNIUS GLAND OF LABEO ROHITA (HAMILTON, 1822), REARED IN LOW, AND HIGH CALCIUM-RICH WATER

Vitamin-D3 induced corpuscles of Stannius gland of Labeo rohita reared in calcium-rich freshwater

  • U Sivagurunathan, dr ICAR-CENTRAL INSTITUTE OF FISHERIES EDUCATION, MUMBAI, INDIA
  • Prem Prakash SRIVASTAVA ICAR-CENTRAL INSTITUTE OF FISHERIES EDUCATION, MUMBAI, INDIA
  • subodh gupta
Keywords: Labeo rohita, Corpuscle of Stannius, Vitamin D3, Calcium enriched water

Abstract

Change in the level of calcium in water affects the Corpuscles of Stannius (CS) in Labeo rohita (Rohu) which was administered by vitamin D3 intra-peritoneally. This study examined the corpuscles of Stannius (CS) gland to the responses of vitamin- D3 intra-peritoneally injected to Labeo rohita @ of 0 IU/kg bw, 100 IU/Kg bw and 500 IU/Kg bw reared in indoor, plastic tubs with 20 and 40 ppm of Calcium (Ca) enriched water. Cellular changes in CS are examined for 15 days with the regular time interval. Rohu administered with 500 IU/kg bw vitamin D3 and exposed to 40ppm Ca-rich water exhibited notable hyperplasia of CS when compared with their control groups which showed normal cell volume. The results demonstrated that the change in calcium level of water modulated the cellular structure of CS to release stanniocalcin and to maintain Ca homeostasis in Labeo rohita which is influenced by intra-peritoneal vitamin D3 and the effect of one-time injection persist up to 15 days of rearing.  In the present study, we have observed the cellular changes in CS due to the synergistic effects of calcium and vitamin D3 which will open a future line of research to understand calcium regulation in vertebrates with special reference to fishes cultured in soft and hard water aquaculture and/ or in saline and sodic soil-water system.

Published
2020-08-25