DIVERSITY AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH THE ALGERIAN MEDICINAL PLANT Artemisia herba alba
The study aimed to isolate, identify and investigate the antibacterial activity of endophytic fungi from the Algerian medicinal plant Artemisia herba alba. The isolation and identification allowed us to get ten (10) fungal strains belonging to four genera: Fusarium, Penicillium, Alternaria, and Aspergillus. The antibacterial activity of the fungi was evaluated by the agar-fungi disk diffusion method against five human pathogenic bacteria. All isolated fungal strains exhibited antibacterial activity against at least one of the bacteria tested with an inhibition zone (IZ) ranging from 7.5 to 25 mm. However, Fusarium sp and Penicillium sp1 have the highest antibacterial activity with an IZ of 23.5 and 25 mm gainst S. aureus and B. subtilis, respectively. Therefore, the secondary metabolites of fungi were extracted after a fermentation by the butanol solvent. The antibacterial activity of the extracts was determined and results showed that Penicillium sp2 has the highest effect against Bacillus subtilis with IZ of 27.3 mm. In conclusion, the present study is the first report about the antimicrobial activity of endophytic fungi residing in A. herba alba. This study helped to prove the use of A. herba alba in traditional medicine. In fact, the fungus Penicillium and Fusarium demonstrated the ability to produce bioactive agents with an antibacterial potential.