MINIMUM INHIBITORY CONCENTRATION OF ANTIMICROBIAL SULFATED POLYSACCHARIDES OF Sargassum cristaefolium AGAINST Bacillus subtilis
Bacterial resistance to antibiotics leads to multiple infections and a decline in curative abilities within the host. Furthermore, preliminary action is needed with natural-based products, as an alternative solution to prevent bacterial growth. The species Sargassum cristaefolium is a marine seaweed currently gaining attention for advanced applications, due to the various intrinsic biological activities, especially as an antimicrobial agent. The aim of this research, therefore, is to determine the minimum inhibitory concentration (MIC) and the selective toxicity of sulfated-polysaccharide extracts obtained from S. cristaefolium, against the gram positive bacteria, Bacillus subtilis. in addition, the efficacy was evaluated using tube dilution test and agar diffusion (disk plate test). The result showed an MIC of 200 ppm for sulfated-polysaccharides, and the least significance difference test demonstrated bacteriostatic selective toxicity. This yield is confirmed to be applicable as a natural and safe antibiotic product, needed for the remediation of numerous bacterial diseases.