ASSESSMENT OF DNA METHYLATION LEVELS IN THE DIFFERENT ORGANS OF SARCOPOTERIUM SPINOSUM (L.) SPACH
DNA methylation at CCGG region plays a vital role in regulating gene expression. Cytosine methylation levels in the four organs of Sarcopoterium spinosum L. Spach, used as folk medicine, were detected and compared using the fluorescent Methylation-Sensitive Amplified Polymorphism (MSAP) technique. Internal cytosine full or hemimethylated (CG) at CCGG were common in all organs. The only one monomorphic region was detected only in the CG methylation type. When organs are compared binary, the leaf-stem has monomorphic regions of 14 (4.8%), leaf-fruit 4 (1.4%), leaf-root 5 (1.7%), fruit-stem 6 (2.1%), fruit-root 2 (0.7%), stem-root 5 (1.7%).These results showed that the similarity levels of CG and CHG methylations of organs are quite low. MSAP ration is the lowest in the fruit (23.9%), while the highest in stem and root (38.4%). The different methylation cases among four organs may be caused by differences in the expression of tissue-specific genes related to cell differentiation and development. However, cloning, sequencing, and uncovering the functions of tissue-specific fragments are needed to verify these results. MSAP is found a highly efficient method for large-scale detection of cytosine methylation, especially for S. spinosum, which is rich in cytosine methylation and do not have genome sequence knowledge.