ANTI-COAGULANT ACTIVITY OF RUBUS ULMIFOLIUS EXTRACTS FROM JIJEL, ALGERIA
The anticoagulants used such as heparins and anti-vitamin K derivatives have life-threatening side effects. Hence, there is an increasing need to identify new anticoagulants from plants, which constitute the major source of drugs for the treatment of many diseases. Such natural substances may act with no side effects for clinical use. Rubus ulmifolius is an Algerian medicinal plant that is used traditionally to cure some diseases. Yet, there is no report regarding its anticoagulant activities. So this work was conducted to study the anticoagulant potential of hydro-methanolic and hydro-ethanolic Rubus ulmifolius extracts through activated partial thromboplastin time (APTT) and prothrombin time (PT) assays. In this study, hydro-methanolic and hydro-ethanolic extracts could significantly prolong the time for the blood to clot estimated by by the APTT assay indicating its ability to inhibit the intrinsic pathway of the coagulation cascade with all concentrations used and the anticoagulant effect persist at an approximate concentration of 195 μ g/mL of plasma. A significant difference was observed between results of the two extracts (p < 0.01) where the hydro- methanolic extract is the best. Perhaps this result is due to the richness of the hydro-methanolic extract with polyphenols compared to the hydro-ethanolic one. The two extracts also affect the extrinsic pathway of coagulation with some concentrations. The combined effect on the two pathways was also observed with some concentrations, which is suggested to cause a qualitative or quantitative fibrinogen defect.