IN VITRO ANTIOXIDANT AND ANTIVIRAL ACTIVITY OF CAMEL MILK CASEIN HYDROLYSATES

  • Farah Ben Abbes, Mrs Laboratory of Applied Microbiology, Faculty of Nature and Life Sciences, Ferhat Abbas Department of Engineering Process, Pharmaceutical Engineering Process, Faculty of Technology, Ferhat Abbas Setif1 University, 19000, Setif, Algeria, University, 19000, Setif, Algeria
  • Rachid Belhattab Ferhat abbas Setif1 University
  • Mohamed Seghier, Dr Enterovirus Laboratory, OMS National Referenced Laboratory, Pasteur Institute, Algiers, Algeria
  • Dahbia Leila Anes-Boulahbal, Pr Enterovirus Laboratory, OMS National Referenced Laboratory, Pasteur Institute, Algiers, Algeria
Keywords: Camel milk casein, pepsin hydrolysates, antioxidant, Coxsackie virus, Vero, A549

Abstract

Camel milk casein and its hydrolysates obtained by pepsin enzyme action for 5 to 180 min, were assessed for antioxidant activities using 2, 2- diphenyl picryl-1- hydrazyl (DPPH), ferric reducing power, total antioxidant capacity (TAC) and b-carotene/linoleic acid bleaching tests. The antiviral effect against Coxsackie virus (Cox B6) was conducted in vitro by using A 549 and Vero cell lines. Coxsackie virus (Cox B6) was first interacted with casein and its hydrolysates at different concentrations, and then mixed with the different cell types. The viable cells were revealed by colorimetric method using neutral red. Results obtained suggest that pepsin camel milk casein hydrolysates can be exploited as a source of antioxidant compounds, and inhibit Coxsackie infection in both cell lines.

Author Biographies

Farah Ben Abbes, Mrs, Laboratory of Applied Microbiology, Faculty of Nature and Life Sciences, Ferhat Abbas Department of Engineering Process, Pharmaceutical Engineering Process, Faculty of Technology, Ferhat Abbas Setif1 University, 19000, Setif, Algeria, University, 19000, Setif, Algeria

PhD student and lecturer at Laghouat University

Mohamed Seghier, Dr, Enterovirus Laboratory, OMS National Referenced Laboratory, Pasteur Institute, Algiers, Algeria

Dr virology

Dahbia Leila Anes-Boulahbal, Pr, Enterovirus Laboratory, OMS National Referenced Laboratory, Pasteur Institute, Algiers, Algeria

Prin virology and Head of the Enterovirus Laboratory

Published
2021-02-03
How to Cite
Ben Abbes, F., Belhattab, R., Seghier, M., & Anes-Boulahbal, D. (2021). IN VITRO ANTIOXIDANT AND ANTIVIRAL ACTIVITY OF CAMEL MILK CASEIN HYDROLYSATES. Journal of Applied Biological Sciences, 15(1), 101-112. Retrieved from http://jabsonline.org/index.php/jabs/article/view/797
Section
Articles