OPTIMIZATION OF PHENOL DEGRADATION BY STREPTOCOCCUS EPIDERMIDIS USING ONE FACTOR AT A TIME METHODOLOGY

Keywords: Bacteria, Degradation, Temperature, Concentration, Phenol

Abstract

This study was aimed at studying the effects of culture conditions of degradation of phenol by Streptococcus epidermidis isolated from soil.Confirmation of the isolate was carried out using standard microbiological procedures. Morphological and biochemical characterizations were employed to identify the isolate. Quantitative phenol was estimated via measure of its absorbance at 510nm wavelength by using UV–visible spectrophotometer and 4-aminoantipyrene as colour indicator. The experiment was repeated with different phenol concentrations to determine the effect of temperature, pH, and inoculum size on degradation of phenol. Percentage degradation (%) was calculated by dividing the reduced amount of phenol with the initial phenol concentration and multiplied by 100. An optimization study that takes into consideration effect of pH, temperature, contact time and inoculum size on the rate of degradation of phenol was carried out. The optimal conditions for the degradation of phenol were found to be pH of 7.0, temperature, of 35oC, inoculum size of 10% and optimum contact time of 24 hours after incubation. This study shows culture conditions such as pH, temperature, contact time and inocula dosage can affect the specific rate of phenol degradation by Streptococcus epidermidis

Published
2021-05-20
How to Cite
Ibrahim, U., Rabah, A., Usman, A., Saleh, A., Oluwatosin, A., & Kawo, A. (2021). OPTIMIZATION OF PHENOL DEGRADATION BY STREPTOCOCCUS EPIDERMIDIS USING ONE FACTOR AT A TIME METHODOLOGY. Journal of Applied Biological Sciences, 15(2), 137-147. Retrieved from http://jabsonline.org/index.php/jabs/article/view/800
Section
Articles