IN VITRO PROPAGATION AND ENCAPSULATION OF GALATELLA ALBANICA DEGEN SHOOT TIPS
Galatella albanica subsp. paparistoi Qosja is a threatened endemic species of Albania observed in small subpopulations in the sandy dunes and serpentine substrates. It is assessed as endangered plant, so stabilization of an efficient micropropagation protocol and development of a method for in vitro preservation via encapsulation of this species is very important. In this investigation, leaf segments were used as explants for tissue culture and various combinations of auxin (NAA) and cytokinin (BAP) were tested as growth regulators for in vitro morphogenesis. Among these combinations, 1 mg l-1 NAA + 0.4 mg l-1 BAP (2.5: 1 ratio) resulted optimal for callus induction, meanwhile the addition of 0.4 mg l-1 NAA : 1 mg l-1 BAP (1 : 2.50 ratio) into culture medium was optimal for adventitious shoots regeneration. Regarding multiplication parameters, the highest number of shootlets, and leaves and shootlets height were recorded with 0.1 mg l-1 NAA : 2 mg l-1BAP containing medium. The in vitro shoot tips were encapsulated in alginate beads and dehydrated in sucrose solution in order to develop an artificial seed method. Maximum of percentage of recovery was observed in non-dehydrated encapsulated shoot tips (96.6%), followed by the dehydration in 0.25 M sucrose solution for 1.5 h and then at 0.5 M sucrose solution for 1.5 h (63.3%). The recovered plantlets from the encapsulated shoot tips were normal and grew well on recovery medium. According to the obtained results, it could be concluded that the developed micropropagation and alginate-encapsulation method of G. albanica can be applied not only for in vitro propagation but also for conservation of its germplasm and collections.