CYTOTOXIC AND ANTITUMORAL EFFECT OF CERATONIA SILIQURACTA L. (CAROB) EXTRACT
Carob (Cerotonia siliqua L.) is a plant with various uses in the field of health among the public. The present study aimed to evaluate carob fruit extract, syrup and essence cytotoxic effects using 3-(4.5-dimethylthiazol-2-yl) methyl thiazole tetrazolium (MTT) bromide test. The study also evaluated apoptotic and necrotic effect using a double staining method of Hoechst 33342 and propidium iodide. Carob fruit extract, syrup and essence were applied to adenocarcinomic human alveolar basal epithelial cells (A549) and mouse adipose tissue derived fibroblast (L929) cells. It was applied in 5 different doses (200 μg/mL, 100 μg/mL, 50 μg/mL, 25 μg/mL, 12.5 μg/mL). According to the results carob extract were not found to be toxic against both L929 cells and A549 cells at administered doses. High degree of toxicity was detected in L929 and A549 cells which carob syrup and essence were directly applied. In high doses, necrosis was found to be high and decreased as it fell. The ratio of apoptosis in L929 cells was higher than that of A549 cells. Further studies should be conducted in different cell lines. Carob is a plant that can be easily cultivated and processed under the conditions of our country. Within the scope of the study, taking into consideration the effects on lung cancer cells; It can be expressed as an alternative approach in treatment with applications.