IN VIVO ANTI-INFLAMMATORY ACTIVITY AND POLYPHENOLIC CONTENT OF AQUEOUS AND ETHANOLIC EXTRACTS OF FICUS CARICA L. FRUIT

Leila Kebal; Nadjet  Mostefa; Noureddine Djebli

Authors

Leila Kebal https://orcid.org/0000-0003-4698-1847
Nadjet MOSTEFA https://orcid.org/0000-0002-1582-0162
Noureddine DJEBLI https://orcid.org/0000-0003-0192-1349

Keywords:

bioactive compounds, mice, histological study, inflammation

Abstract

The present study aims at studying the phytochemical compounds and evaluating in vivo the anti-inflammatory effect of aqueous and ethanolic extracts of Ficus carica L. fruit. The total contents of phenolics, flavonoids, and condensed tannins were determined spectrophotometrically using Folin-Ciocalteu, aluminum trichloride and vanillin reagents respectively. The in vivo anti-inflammatory activity was realized by paw edema model in mice induced by intraplantar injection of λ-carrageenan and treated with doses of each extract at 250, 350, and 500 mg/kg body weight. Paws’ tissues were subjected to histological study to devote the effect of extracts at the tissue scale. Preliminary phytochemical screening indicates a significant appearance of flavonoids, tannins, alkaloids and steroids in both types of extracts. The aqueous extract marks the highest values in total phenolics, total flavonoids and condensed tannins contents: 951.06±61.08 mg GAE/100g dry weight (DW), 428.34±15.42 mg QE/100g DW and 474.07±50.25 mg CE/100g DW respectively. Our results show that both treatments with aqueous and ethanolic extract present a significant inhibition ^*P˂0.05 of the edema in a dose-dependent manner compared to the standard group (treated with 2-[2-(2,6-dichloroanilino) phenyl]acetic acid as anti-inflammatory medicine) during the whole experiment period. This was confirmed histologically by the observation of a less intense inflammatory infiltrate as the dose of extracts increase. This study reveals a pronounced anti-inflammatory effect in Ficus carica L. fruit extracts which could be related to the high amounts of phenolic compounds. Thus, fruit can be a promising natural substitute therapeutic to treat inflammation.

References

Iwalewa, E. O., Mc-Gaw, L. J., Naidoo, V., Eloff, J. N. (2007): Inflammation: The foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. African Journal of Biotechnology 6(25): 2868–2885. https://doi.org/10.5897/AJB2007.000-2457

Silva da Rosa, H., Santos, M. C., Costa, M. T., Salgueiro, A., Duarte da Silva, M., Nogueira-Librelotto, D. R., Jesse, C., Machado, M. M., Souza de Oliveira, L. F., Folmer, V., Mendez, A. S. L. (2022): Sida tuberculata: In vitro cytotoxicity and in vivo anti-inflammatory effect. Journal of Ethnopharmacology 287: 114-956. https://doi.org/10.1016/j.jep.2021.114956

Lee, C. Y., Park, S. H., Lim, H. Y., Jang, S. G., Park, K. J., Kim, D. S., Kim, J. H., Cho, J. Y. (2021): In vivo anti-inflammatory effects of Prasiola japonica ethanol extract. Journal of Functional Foods 80: 104-440. https://doi.org/10.1016/j.jff.2021.104440

Qamar, M., Akhtar, S., Ismail, T., Yuan, Y., Ahmad, N., Tawab, A., Ismail, A., Barnard, R. T., Cooper, M. A., Blaskovich, M. A. T., Ziora, Z. M. (2021): Syzygium cumini(L.),Skeels fruit extracts: In vitro and in vivo anti-inflammatory properties. Journal of Ethnopharmacology 271: 113-805. https://doi.org/10.1016/j.jep.2021.113805

Tauchen, J., Bortl, L., Huml, L., Miksatkova, P., Doskocil, I., Marsik, P., Villegas, P. P. P., Flores, Y. B., Damme, P. V., Lojka, B., Havlik, J., Lapcik, O., Kokoska, L. (2016): Phenolic composition, antioxidant and anti-proliferative activities of edible and medicinal plants from the Peruvian Amazon. Revista Brasileira de Farmacognosia 26(6): 728–737. https://doi.org/10.1016/j.bjp.2016.03.016

Taïbi, K., Aït Abderrahim, L., Boussaid, M., Taibi, F., Achir, M., Souana, K., Benaissa, T., Farhi, K. H., Naamani, F. Z., Nait Said, K. (2021): Unraveling the ethnopharmacological potential of medicinal plants used in Algerian traditional medicine for urinary diseases. European Journal of Integrative Medicine 44: 101-339. https://doi.org/10.1016/j.eujim.2021.101339

Lo Turco, V., Potortì, A. G., Tropea, A., Dugo, G., Di Bella, G. (2020): Element analysis of dried figs (Ficus carica L.) from the Mediterranean areas. Journal of Food Composition and Analysis 90: 103-503. https://doi.org/10.1016/j.jfca.2020.103503

Abdel-Rahman, R., Ghoneimy, E., Abdel-Wahab, A., Eldeeb, N., Salem, M., Salama, E., Ahmed, T. (2021): The therapeutic effects of Ficus carica extract as antioxidant and anticancer agent. South African Journal of Botany 141: 273–277. https://doi.org/10.1016/j.sajb.2021.04.019

Patil, V., Patil, V. (2011): Evaluation of anti-inflammatory activity of Ficus carica linn. Leaves. Indian Journal of Natural Products and Resources 2(2): 151-155.

Amessis-Ouchemoukh, N., Ouchemoukh, S., Meziant, N., Idiri, Y., Hernanz, D., Stinco, C. M., Rodríguez-Pulido, F. J., Heredia, F. J., Madani, K., Luis, J. (2017): Bioactive metabolites involved in the antioxidant, anticancer and anticalpain activities of Ficus carica L., Ceratonia siliqua L. and Quercus ilex L. extracts. Industrial Crops and Products 95: 6–17. https://doi.org/10.1016/j.indcrop.2016.10.007

Gilani, A. H., Mehmood, M. H., Janbaz, K. H., Khan, A., Saeed, S. A. (2008): Ethnopharmacological studies on antispasmodic and antiplatelet activities of Ficus carica. Journal of Ethnopharmacology 119(1): 1–5. https://doi.org/10.1016/j.jep.2008.05.040

Palmeira, L., Pereira, C., Dias, M. I., Abreu, R. M. V., Corrêa, R. C. G., Pires, T. C. S. P., Alves, M. J., Barros, L., Ferreira, I. C. F. R. (2019): Nutritional, chemical and bioactive profiles of different parts of a Portuguese common fig (Ficus carica L.) variety. Food Research International 126: 108-572. https://doi.org/10.1016/j.foodres.2019.108572

Francini, A., Pintado, M., Manganaris, G. A., Ferrante, A. (2020): Editorial: Bioactive Compounds Biosynthesis and Metabolism in Fruit and Vegetables. Frontiers in Plant Science 11: 129. https://doi.org/10.3389/fpls.2020.00129

Slatnar, A., Klancar, U., Stampar, F., Veberic, R. (2011): Effect of Drying of Figs (Ficus carica L.) on the Contents of Sugars, Organic Acids, and Phenolic Compounds. Journal of Agricultural and Food Chemistry 59(21): 11696–11702. https://doi.org/10.1021/jf202707y

Bey, M. B., Louaileche, H., Zemouri, S. (2013): Optimization of phenolic compound recovery and antioxidant activity of light and dark dried fig (Ficus carica L.) varieties. Food Science and Biotechnology 22(6): 1613–1619. https://doi.org/10.1007/s10068-013-0258-7

Rtibi, K., Grami, D., Wannes, D., Selmi, S., Amri, M., Sebai, H., Marzouki, L. (2018): Ficus carica aqueous extract alleviates delayed gastric emptying and recovers ulcerative colitis-enhanced acute functional gastrointestinal disorders in rats. Journal of Ethnopharmacology 224: 242–249. https://doi.org/10.1016/j.jep.2018.06.001

Aiyegoro, O. A., Okoh, A. I. (2010): Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine 10(1): 21. https://doi.org/10.1186/1472-6882-10-21

Gacem, M. A., Telli, A., Gacem, H., Ould-El-Hadj-Khelil, A. (2019): Phytochemical screening, antifungal and antioxidant activities of three medicinal plants from Algerian steppe and Sahara (preliminary screening studies). SN Applied Sciences 1(12): 1721. https://doi.org/10.1007/s42452-019-1797-1

Singleton, V. L., Rossi, J. A. (1965): Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture 16(3): 144-158.

Huang, D. J., Lin, C. D., Chen, H. J., Lin, Y. H. (2004): Antioxydant and antiproliferative activities of sweet potato (Ipomoea batatas L. Lam ‘Tainong 57’) constituents. Botanical Bulletin of Academia Sinica 45: 179–186.

Julkunen-Tiitto, R. (1985): Phenolic constituents in the leaves of northern willows: Methods for the analysis of certain phenolics. Journal of Agricultural and Food Chemistry 33(2): 213–217. https://doi.org/10.1021/jf00062a013

OECD. (2008): Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure. OECD. https://doi.org/10.1787/9789264071049-en

Loomis, T. A., Hayes, A. W. (1996): Loomis’s essentials of toxicology. 4th ed. San Diego Academic Press, pp. 208- 245.

Winter, C. A., Risley, E. A., Nuss, G. W. (1962): Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Antiinflammatory Drugs. Experimental Biology and Medicine 111(3): 544–547. https://doi.org/10.3181/00379727-111-27849

Ndiaye Sy, A., Fall, A. D., Ndiaye, M., Sall, A. O., Sy, G. Y., Bassène, E., Dièye, A. M. (2016): Mise en évidence de l’activité anti-inflammatoire des sous-fractions méthanoliques des feuilles de Moringa oleifera Lam. (Moringaceae) chez le rat. International Journal of Biological and Chemical Sciences 10(2): 760-768. https://doi.org/10.4314/ijbcs.v10i2.25

Alqethami, A., Aldhebiani, A. Y. (2021): Medicinal plants used in Jeddah, Saudi Arabia: Phytochemical screening. Saudi Journal of Biological Sciences 28(1): 805–812. https://doi.org/10.1016/j.sjbs.2020.11.013

Oszmiański, J., Lachowicz, S., Gorzelany, J., Matłok, N. (2018): The effect of different maturity stages on phytochemical composition and antioxidant capacity of cranberry cultivars. European Food Research and Technology 244(4): 705–719. https://doi.org/10.1007/s00217-017-2994-z

Yang, X., Yu, W., Ou, Z., Ma, H., Liu, W., Ji, X. (2009): Antioxidant and Immunity Activity of Water Extract and Crude Polysaccharide from Ficus carica L. Fruit. Plant Foods for Human Nutrition 64(2): 167–173. https://doi.org/10.1007/s11130-009-0120-5

Bey, M. B., Louaileche, H. (2015): A comparative study of phytochemical profile and in vitro antioxidant activities of dark and light dried fig (Ficus carica L.) varieties. The Journal of Phytopharmacology 4(1): 41-48.

Pourghayoumi, M., Bakhshi, D., Rahemi, M., Noroozisharaf, A., Jafari, M., Salehi, M., Chamane, R., Hernandez, F. (2016): Phytochemical Attributes of Some Dried Fig (Ficus carica L.) Fruit Cultivars Grown in Iran. Agriculturae Conspectus Scientificus 81(3): 161–166.

Debib, A., Tir-Touil, M. A., Meddah, B., Hamaidi-Chergui, F., Menadi, S., Alsayadi, M. S. (2018): Evaluation of antimicrobial and antioxidant activities of oily macerates of Algerian dried figs (Ficus carica L.). International Food Research Journal 25(1): 351-356.

Mopuri, R., Ganjayi, M., Meriga, B., Koorbanally, N. A., Islam, M. S. (2018): The effects of Ficus carica on the activity of enzymes related to metabolic syndrome. Journal of Food and Drug Analysis 26(1): 201–210. https://doi.org/10.1016/j.jfda.2017.03.001

Debib, A., Tir-Touil, A., Mothana, R. A., Meddah, B., Sonnet, P. (2014): Phenolic Content, Antioxidant and Antimicrobial Activities of Two Fruit Varieties of Algerian Ficus carica L: Antioxdant and Antimicrobial Activity of Algerian Figs. Journal of Food Biochemistry 38(2): 207–215. https://doi.org/10.1111/jfbc.12039

Belguith-Hadriche, O., Ammar, S., Contreras, M. M., Turki, M., Segura-Carretero, A., El Feki, A., Makni-Ayedi, F., Bouaziz, M. (2016): Antihyperlipidemic and Antioxidant Activities of Edible Tunisian Ficus carica L. Fruits in High Fat Diet-Induced Hyperlipidemic Rats. Plant Foods for Human Nutrition 71(2): 183–189. https://doi.org/10.1007/s11130-016-0541-x

Benmaghnia, S., Meddah, B., Tir-Touil, A., Gabaldon-Hernandez, J. A. (2019): Phytochemical analysis, antioxidant and antimicrobial activities of three samples of dried figs (Ficus carica L.) from the region of Mascara (Western Algeria). Journal of Microbiology, Biotechnology and food sciences 9(2): 208-215. https://doi.org/10.15414/jmbfs.2019.9.2.208-215

Ahmad, S., Bhatti, F. R., Khaliq, F. H., Irshad, S., Madni, A. (2013): A review on the prosperous phytochemical and pharmacological effects of Ficus carica. International Journal of Bioassays 2(5): 843–849.

Cuzzocrea, S., Zingarelli, B., Hake, P., Salzman, A. L., Szabo, C. (1998): Antiinflammatory Effects of Mercaptoethylguanidine, a Combined Inhibitor of Nitric Oxide Synthase and Peroxynitrite Scavenger, in Carrageenan-induced Models of Inflammation. Free Radical Biology and Medicine 24(3): 450–459. https://doi.org/10.1016/S0891-5849(97)00280-3

Gilroy, D. W., Colville-Nash, P. R., Willis, D., Chivers, J., Paul-Clark, M. J., Willoughby, D. A. (1999): Inducible cyclooxygenase may have anti-inflammatory properties. Nature Medicine 5(6): 698–701. https://doi.org/10.1038/9550

Chauhan, P., Singh, S., Gupta, Y., Kumar, U. (2018): Evaluation of toxicity studies and anti-inflammatory activity of Terminalia Bellerica in carrageenan-induced paw edema in experimental rats. Journal of Natural Science, Biology and Medicine 9(2): 169-174. https://doi.org/10.4103/jnsbm.JNSBM_159_17

Zakaria, Z. A., Hussain, M. K., Mohamad, A. S., Abdullah, F. C., Sulaiman, M. R. (2012): Anti-Inflammatory Activity of the Aqueous Extract of Ficus Deltoidea. Biological Research For Nursing 14(1): 90–97. https://doi.org/10.1177/1099800410395378

Rasool, S., Uttra, A. M., Uttra, M. M., Ahmed, F., Abbas, K. (2020): Evaluation of Anti-Inflammatory and Analgesic Activities of Methanolic Extract of Aitchisonia Rosea in Albino Mice. RADS Journal of Pharmacy and Pharmaceutical Sciences 8(1): 31–36. https://doi.org/10.37962/jpps.v8i1.371

Lee, C. Y., Park, S. H., Lim, H. Y., Jang, S. G., Park, K. J., Kim, D. S., Kim, J. H., Cho, J. Y. (2021): In vivo anti-inflammatory effects of Prasiola japonica ethanol extract. Journal of Functional Foods 80: 104-440. https://doi.org/10.1016/j.jff.2021.104440

Mansour, S., Djebli, N., Ozkan, E. E., Mat, A. (2014): In vivo antiinflammatory activity and chemical composition of Hypericum scabroides. Asian Pacific Journal of Tropical Medicine 7: 514–520. https://doi.org/10.1016/S1995-7645(14)60283-2

Singh, S., Tomar, A., Chandel, H. S. (2012): Anti-inflammatory effect of hydroalcoholic extract of fruit of Ficus carica. International Journal of Drug Research and Technology 2(6): 440-445.

Ali, B., Mujeeb, M., Aeri, V., Mir, S. R., Faiyazuddin, M., Shakeel, F. (2012): Anti-inflammatory and antioxidant activity of Ficus carica Linn. Leaves. Natural Product Research 26(5): 460–465. https://doi.org/10.1080/14786419.2010.488236

Eteraf-Oskouei, T., Allahyari, S., Akbarzadeh-Atashkhosrow, A., Delazar, A., Pashaii, M., Gan, S. H., Najafi, M. (2015): Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation. Evidence-Based Complementary and Alternative Medicine 2015: 1–9. https://doi.org/10.1155/2015/760405

Tungmunnithum, D., Thongboonyou, A., Pholboon, A., Yangsabai, A. (2018): Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines 5(3): 93. https://doi.org/10.3390/medicines5030093

Zhang, T., Qiu, F., Chen, L., Liu, R., Chang, M., Wang, X. (2021): Identification and in vitro anti-inflammatory activity of different forms of phenolic compounds in Camellia oleifera oil. Food Chemistry 344: 128-660. https://doi.org/10.1016/j.foodchem.2020.128660

Bouhlali, E. D. T., Hmidani, A., Bourkhis, B., Khouya, T., Ramchoun, M., Filali-Zegzouti, Y., Alem, C. (2020): Phenolic profile and anti-inflammatory activity of four Moroccan date (Phoenix dactylifera L.) seed varieties. Heliyon 6(2): e03436. https://doi.org/10.1016/j.heliyon.2020.e03436.

Mawa, S., Husain, K., Jantan, I. (2013): Ficus carica L. (Moraceae): Phytochemistry, Traditional Uses and Biological Activities. Evidence-Based Complementary and Alternative Medicine 2013: 1–8. https://doi.org/10.1155/2013/974256

IN VIVO ANTI-INFLAMMATORY ACTIVITY AND POLYPHENOLIC CONTENT OF AQUEOUS AND ETHANOLIC EXTRACTS OF FICUS CARICA L. FRUIT

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