PROTECTIVE EFFECT OF ALGERIAN SALVIA VERBENACA EXTRACT AGAINST INFLAMMATION AND OXIDATIVE STRESS
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Salvia verbenaca L., polyphenols, anti-inflammatory effect, antioxidant effectAbstract
This study aims to examine the anti-inflammatory and antioxidant effects of polyphenolic extract of Salvia verbenaca L. using in vivo and in vitro models. The in vivo anti-inflammatory activity was evaluated using carrageenan-induced mice paw edema method. The in vivo antioxidant effect of the extract was explored by measuring oxidative stress (MDA, CAT, GSH and SOD) parameters. The in vitro anti-inflammatory activity was performed using protein denaturation and membrane stabilization assays. DPPH and hydrogen peroxide tests were used for the in vitro antioxidant evaluation. The highest percentage of edema inhibition was 75.03% at the concentration of 500 mg/kg. Significant increases (p˂0.05) in the activities of CAT, SOD, GSH and significant decreases in the MDA level activity were showed. The extract was found to possess an in vitro anti‐inflammatory activity by inhibiting the heat induced protein denaturation and red blood cells membrane stabilization with the IC50 values of 133.21±0.89 and 160.62±1.60 μg/mL, respectively. This study demonstrated that extract exhibited high free radical scavenging activity as showed by the low IC50 values for DPPH (115.41±1.40 μg/mL) and for H2O2 (180.39±1.77 μg/mL) scavenging assays. Our findings suggest that Salvia verbenaca could be tested as a drug candidate against oxidative stress and inflammation diseases.
References
Jing, W., Zhong, J., Ping, L. J., Yan, L. H. (2018): Relationship between oxidative stress and inflammation in peripheral and cerebral system of oxonate-induced hyperuricemic rats. Brazilian Journal of Pharmaceutical Sciences 53 (4): e00229.
Hussain, A., Aslam, B., Muhammad, F., Faisal, M. N. (2022): In vitro Antioxidant Activity and In vivo Anti-inflammatory Effect of Ricinus communis (L.) and Withania somnifera (L.) hydroalcoholic extracts in rats. Brazilian Archives of Biology and Technology 64: e21200783
Biswas, S., Das, R., Banerjee, E. R. (2017): Role of free radicals in human inflammatory diseases. Aims Biophysics 4(4): 596-614.
Forman, H. J., Zhang, H. (2021): Targeting oxidative stress in disease: Promise and limitations of antioxidant therapy. Nature Reviews Drug Discovery 20(9): 689-709.
Akimat, E. K., Omwenga, G. I., Moriasi, G. A., Ngugi, M. P. (2021): Antioxidant, Anti-Inflammatory, Acute Oral Toxicity, and Qualitative Phytochemistry of The Aqueous Root Extract of Launaea cornuta (Hochst. Ex Oliv. & Hiern.). Journal of Evidence-Based Integrative Medicine 26: 2515690X211064585.
Bindu, S., Mazumder, S., Bandyopadhyay, U. (2020): Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: A current perspective. Biochemical Pharmacology 180: 11414.
Martínez-Francés, V., Hahn, E., Ríos, S., Rivera, D., Reich, E., Vila, R., Cañigueral, S. (2017): Ethnopharmacological and chemical characterization of Salvia Species used in valencian traditional herbal preparations. Frontiers in Pharmacology 8(467).
Dweck, A.C. (2000): The folklore and cosmetic use of various Salvia species. In: S.E. Kintzios Sage: the genus Salvia. CRC Press, London,
Kelen, M., Tepe, B. (2008): Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Bioresource Technology 99(10): 4096-4104.
González-Chávez, M.M., Ramos-Velázquez, C, S., Serrano-Vega, R., Pérez-González, C., Sánchez-Mendoza, E., Pérez-Gutiérrez, S. (2017): Anti-inflammatory activity of standardized dichloromethane extract of Salvia connivens on macrophages stimulated by LPS. Pharmaceutical Biology 55(1): 1467-1472.
Guaouguaou, F. E., Taghzouti, K., Oukabli, M., Masrar, A., Chabraoui, L., Bouabdellah, M., Es-Safi, N. E. (2019): Acute and subchronic oral and dermal toxicological studies of salvia verbenaca extracts in mice and rats. Journal of Herbs. Spices & Medicinal Plants 25(1): 33-42.
Fiore, G., Massarelli, P., Sajeva, M., Francji, G.G. (2012): Anti-tumor activity of the methanolic extracts of Salvia menthifolia. Revista Brasileira de Farmacognosia 22(381-387.
Jiang, Y., Zhang, L., Rupasinghe, H. P. V. (2017): Antiproliferative effects of extracts from Salvia officinalis L. and Saliva miltiorrhiza Bunge on hepatocellular carcinoma cells. Biomedicine & Pharmacotherapy 85:57-67.
Šmidling, D., Mitić-Ćulafić, D., Vuković-Gačić, B., Simić, D., Knežević-Vukčević, J. (2008): Evaluation of antiviral activity of fractionated extracts of sage Salvia officinalis L. (Lamiaceae). Archives of Biological Sciences 60:421-429.
Kamatou, G.P.P., Van Vuuren, S. F., Van Heerden, R. F., Seaman, T., Viljoen, A. M. (2007): Antibacterial and antimycobacterial activities of South African Salvia species and isolated compounds from S. chamelaeagnea. South African Journal of Botany 73(4): 552-557.
Kivrak, İ. Duru, M. E., Öztürk, M., Mercan, N., Harmandar, M., Topçu, G. (2009): Antioxidant, anticholinesterase and antimicrobial constituents from the essential oil and ethanol extract of Salvia potentillifolia. Food Chemistry 116(2): 470-479.
Yu, J., Ahmedna, M., Goktepe, I. (2005): Effects of processing methods and extraction solvents on concentration and antioxidant activity of peanut skin phenolics. Food Chemistry 90(1): 199-206.
Othman, A., Ismail, A., Abdul Ghani, N., Adenan, I. (2007): Antioxidant capacity and phenolic content of cocoa beans. Food Chemistry 100(4): 1523-1530.
Bahorun, T., Gressier, B., Trotin, F., Brunet, C., Dine, T., Luyckx, M., Vasseur, J., Cazin, M., Cazin, J. C., Pinkas, M. (1996): Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittelforschung 46(11): 1086-1089.
Winter, C.A., Risley, E. A., Nuss, G.W. (1962): Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine 111:544–547.
Iqbal, M., Sharma, S, D., Okazaki, Y., Fujisawa, M., Okada, S. (2003): Dietary supplementation of curcumin enhances antioxidant and phase II metabolizing enzymes in ddY male mice: Possible role in protection against chemical carcinogenesis and toxicity. Pharmacology & Toxicology 92(1): 33-38.
Ohkawa, H., Ohishi, N., Yagi, K. (1979): Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95(2): 351-358.
Clairborne, A., (1985): Catalase activity. In: R.A. Greenwald CRP Handbook of Methods for Oxygen Radical Research. CRP Press, Boca Raton, FL,
Ellman, G.L., (1959): Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics 82(1): 70-77.
Beauchamp, C., Fridovich, I. (1971): Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44(1): 276-287.
Mizushima, Y., Kobayashi, M. (1968): Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. Journal of Pharmacy and Pharmacology 20(3): 169-173.
Sakat, S., Wankhede, S., Juvekar, M., Gambhire, M. (2010): In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharmacy and Pharmaceutical Sciences 2:146-155.
Shinde, U.A., Phadke, A. S., Nair, A. M., Mungantiwar, A.A., Dikshit, V. J., Saraf, M. N. (1999): Membrane stabilizing activity — a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia 70(3): 251-257.
Brand-Williams, W., Cuvelier, M. E., Berset, C. (1995): Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology 28(1): 25-30.
Najmi, A., Javed, S. A., Al Bratty, M., Alhazmi, H. A. (2022): Modern approaches in the discovery and development of plant-based natural products and their analogues as potential therapeutic agents. Molecules 27(2): 349.
Albarakati, A. J. A. (2022): Protocatechuic acid counteracts oxidative stress and inflammation in carrageenan-induced paw edema in mice. Environmental Science and Pollution Research 1-10.
Yimer, T., Birru, E. M., Adugna, M., Geta, M., Emiru, Y.K. (2020): Evaluation of analgesic and anti-inflammatory activities of 80% methanol root extract of Echinops kebericho M. (Asteraceae). Journal of Inflammation Research 13:647-658.
Azam, S., Huda, A., Shams, K., Ansari, P., Mohamed, K., Hasan, M., Azad, A., Mondal, K., Zaouad, K. (2015): Anti-inflammatory and anti-Oxidant study of ethanolic extract of Mimosa pudica. Journal of Young Pharmacists 7:234-240.
Umamageswari, A., Kudagi, B. (2015): Anti-inflammatory and analgesic properties of Ocimum sanctum: a comparative study using animal models. International Journal of Basic and Clinical Pharmacology 4(5): 981-986.
Boussouf, L., Boutennoune, H., Kebieche, M., Adjeroud, N., Al-Qaoud, K., Madani, K. (2017): Anti-inflammatory, analgesic and antioxidant effects of phenolic compound from Algerian Mentha rotundifolia L. leaves on experimental animals. South African Journal of Botany 113:77-83.
Boutennoun, H., Boussouf, L., Kebieche, M., Al-Qaoud, K., Madani, K. (2017): In vivo analgesic, anti-inflammatory and antioxidant potentials of Achillea odorata from north Algeria. South African Journal of Botany 112:307-313.
BenSaad, L. A., Kim, K. H., Quah, C. C., Kim, W. R., Shahimi, M. (2017): Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum. BMC Complementary and Alternative Medicine 17(1): 1-10.
Al-Khayri, J. M., Sahana, G. R., Nagella, P., Joseph, B. V., Alessa, F. M., Al-Mssallem, M. Q. (2022): flavonoids as potential anti-Inflammatory molecules: A Review. Molecules 27(9): 2901.
Ben Khedir, S., Mzid, M., Bardaa, S., Moalla, D., Sahnoun, Z., Rebai, T. (2016): In vivo evaluation of the anti-inflammatory effect of Pistacia lentiscus fruit oil and its effects on oxidative stress. Evidence-Based Complementary and Alternative Medicine 2016: 1-12.
Zhang, H., Shang, C., Tian, Z., Amin, H. K., Kassab, R. B., Abdel Moneim, A. E., Zhang, Y. (2020): Diallyl disulfide suppresses inflammatory and oxidative machineries following carrageenan injection-induced paw edema in mice. Mediators of inflammation 2020: 1-11
Ginwala, R., Bhavsar, R., Chigbu, D. G. I., Jain, P., Khan, Z. K. (2019): Potential role of flavonoids in treating chronic inflammatory diseases with a special focus on the anti-inflammatory activity of apigenin. Antioxidants 8(2): 35.
Ullah, A., Munir, S., Badshah, S. L., Khan, N., Ghani, L., Poulson, B. G., Jaremko, M. (2020): Important flavonoids and their role as a therapeutic agent. Molecules 25(22): 5243.
Xian, D., Guo, M., Xu, J., Yang, Y., Zhao, Y., Zhong, J. (2021): Current evidence to support the therapeutic potential of flavonoids in oxidative stress-related dermatoses. Redox Report 26(1): 134-146.
Gunathilake, K. D. P. P., Ranaweera, K. K. D. S., Rupasinghe, P. V. (2018): In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines 6(4): 107.
Ngoua Meye Misso, R. L., Ndong, J. D. L. C., Sima Obiang, C., Ondo, J. P., Ndong Atome, G. R., Ovono Abessolo, F., Obame-Engonga, L. C. (2018): Phytochemical studies, antiangiogenic, anti-inflammatory and antioxidant activities of Scyphocephalium ochocoa Warb. (Myristicaceae), medicinal plant from Gabon. Clinical Phytoscience 4:1-13.
Anoop, M. V., Bindu, A R. (2015): In vitro antiinflammatory studies on Syzigium zeylanicum (L) DC leaves. International Journal of Pharma Research & Review 4:18-27.
Ranasinghe, P., Ranasinghe, P., Abeysekera, W. K. M., Premakumara, G. S., Perera, Y. S., Gurugama, P., Gunatilake, S. (2012): In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts. Pharmacognosy Research 4:196-202.
Labu, Z., Laboni, F., Tarafder, M., Harun, M. (2015): Membrane stabilization as a mechanism of anti-Inflammatory and thrombolytic activities of ethanolic extract of arial parts of Spondiasis pinanata (Family: Anacardiaceae). Pharmacology & Pharmacy 2:44-51.
Tepe, B., Eminagaoglu, O., Akpulat, H. A., Aydin, E. (2007): Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia verticillata (L.) subsp. verticillata and S. verticillata (L.) subsp. amasiaca (Freyn & Bornm.) Bornm. Food Chemistry 100(3): 985-989.
Belkhiri, F., Baghiani, A., Zerroug, M. M., Arrar, L. (2017): Investigation of antihemolytic, xanthine oxidase inhibition, antioxidant and antimicrobial properties of Salvia verbenaca L. aerial part extracts. African Journal of Traditional, Complementary and Alternative Medicines 14(2): 273-281.
Silva, C., Herdeiro, R., Mathias, C., Panek, A., Silveira, C., Rodrigues, V., Rennó, M., Falcão, D., Malta Cerqueira, D., Minto, A., Nogueira, F., Quaresma, C., Silva, J., Boylan, F., Eleutherio, E. (2005): Evaluation of antioxidant activity of Brazilian plants. Pharmacological Research 52:229-33.
Villaño, D., Fernández-Pachón, M. S., Moyá, M. L., Troncoso, A. M., García-Parrilla, M. C. (2007): Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta 71(1): 230-235.
Farhat, M., Hamada, R., Sotomayor, J., Landoulsi, A., Jordán, M. (2015): Antioxidant properties and evaluation of phytochemical composition of Salvia verbenaca L. extracts at different developmental stages. Plant Foods for Human Nutrition 70:15-20.
Mamache, W., Amira, S., Chawki, B., Laouer, H., Benchikh, F. (2020): In vitro antioxidant, anticholinesterases, anti-α-amylase, and anti-α-glucosidase effects of Algerian Salvia aegyptiaca and Salvia verbenaca. Journal of Food Biochemistry 44: e13472.
Albayrak, S., Aksoy, A., Sagdic, O., Hamzaoglu, E. (2010): Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey. Food Chemistry 119(1): 114-122.
Baba, S.A. Malik, S. A. (2015): Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science 9(4): 449-454.
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