NEUTROPHIL-INFILTRATING REGULATORY POTENTIAL OF Lactobacillus pentosus 124-2 IN ACUTE INFLAMMATORY MODEL
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Neutrophils, inflammation, lactobacillus, carrageenan, oedemaAbstract
Neutrophils are often responsible for pathological inflammation, as well as other immunological responses. Controlling their activity is crucial to maintaining host integrity, particularly preventing against chronic inflammation. Inflammatory oedema was induced in paw of male Wistar rats using 1% iota-carrageenan. Control group was neither induced nor treated with lactic acid bacteria (LAB), while negative control group was induced without treatment. Experimental group was orogastrically treated with Lactobacillus pentosus 124-2 (5x107 CFU/mL), while positive control group received diclofenac sodium treatment (150 mg/kg body weight) following carrageenan administration. Biochemical assays including myeloperoxidase (MPO), malondialdehyde (MDA), and nitric oxide (NO) were performed on the blood samples at t=1, 4, 24, and 72 hours, and tissue histology was done using standard methods to monitor neutrophil infiltration. LAB treated group showed marked decrease in NO and MPO activity at 4 hours following inducement as well as increase in MDA activity till 72 hours (p<0.05). Neutrophil infiltration was markedly controlled in the LAB treated group between 4 to 72 hours and compared favorably with the diclofenac sodium. This inflammatory model established that administered L. pentosus 124-2 inhibited neutrophil infiltration and activation resulting in the significant MPO reduction, while also regulating NO production.
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