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Collagen, antioxidant, wound healing, skin irritation


The imbalance of reactive oxygen species (ROS) may cause oxidative stress which is harmful to skin health. Moreover, oxidative stress could also impair the healing of a wound. Antioxidants have been demonstrated their benefits to skin health and wound healing. Previous studies have shown collagen tripeptide has beneficial effects on skins, such as alleviating photoaging, improving skin elasticity and hydration, improving type I collagen synthesis and more. In this study, anti-oxidant activities and in vitro wound healing effects of Meta collagen (Highly content dipeptide and tripeptide) were investigated. The skin irritation test of Meta collagen was also studied. In the DPPH scavenging assay, Meta collagen could scavenge DPPH dose-dependently and showed the anti-oxidant activity. Meta collagen could enhance the migration of NIH-3T3 fibroblasts, suggesting its in vitro wound healing effects. Meanwhile, Meta collagen (1000 mg/mL) is considered as non-irritant to skin by using a reconstructed human epidermis. According to these results, Meta collagen shows anti-oxidant activities and in vitro wound healing effects without skin irritation. Meta collagen might be a potential material to be developed as a skin care product in the future.


Comino-Sanz, I. M., Lopez-Franco, M. D., Castro, B., and Pancorbo-Hidalgo, P. L. (2021): The role of antioxidants on wound healing: A review of the current evidence. Journal of Clinical Medicine 10: 3558.

Chen, J., Liu, Y., Zhao, Z., and Qiu, J. (2021): Oxidative stress in the skin: Impact and related protection. International Journal of Cosmetic Science 43: 495-509.

Di Meo, S., Reed, T. T., Venditti, P., and Victor, V. M. (2016): Role of ros and rns sources in physiological and pathological conditions. Oxidative Medicine and Cellular Longevity 2016: 1245049.

Scharffetter-Kochanek, K., Brenneisen, P., Wenk, J., Herrmann, G., Ma, W., Kuhr, L., et al. (2000): Photoaging of the skin from phenotype to mechanisms. Experimental Gerontology 35: 307-316.

Dixon, S. J. and Stockwell, B. R. (2014): The role of iron and reactive oxygen species in cell death. Nature Chemical Biology 10: 9-17.

Butnariu, M. and Samfira, I. (2012): Free radicals and oxidative stress. Bioequivalence & Bioavailability 4(6): 4-6.

Michalak, M. (2022): Plant-derived antioxidants: Significance in skin health and the ageing process. International Journal of Molecular Sciences 23: 585.

Rosenbaum, A. J., Banerjee, S., Rezak, K. M., and Uhl, R. L. (2018): Advances in wound management. Journal of the American Academy of Orthopaedic Surgeons 26: 833-843.

Velnar, T., Bailey, T., and Smrkolj, V. (2009): The wound healing process: An overview of the cellular and molecular mechanisms. Journal of International Medical Research 37: 1528-1542.

Meephansan, J., Rungjang, A., Yingmema, W., Deenonpoe, R., and Ponnikorn, S. (2017): Effect of astaxanthin on cutaneous wound healing. Clinical, Cosmetic and Investigational Dermatology 10: 259-265.

Kunkemoeller, B. and Kyriakides, T. R. (2017): Redox signaling in diabetic wound healing regulates extracellular matrix deposition. Antioxidants & Redox Signaling 27: 823-838.

Fitzmaurice, S. D., Sivamani, R. K., and Isseroff, R. R. (2011): Antioxidant therapies for wound healing: A clinical guide to currently commercially available products. Skin Pharmacology and Physiology 24: 113-126.

Farazin, A., Shirazi, F. A., and Shafiei, M. (2023): Natural biomarocmolecule-based antimicrobial hydrogel for rapid wound healing: A review. International Journal of Biological Macromolecules 244: 125454.

Pyun, H. B., Kim, M., Park, J., Sakai, Y., Numata, N., Shin, J. Y., et al. (2012): Effects of collagen tripeptide supplement on photoaging and epidermal skin barrier in uvb-exposed hairless mice. Preventive Nutrition and Food Science 17: 245-253.

Choi, S. Y., Ko, E. J., Lee, Y. H., Kim, B. G., Shin, H. J., Seo, D. B., et al. (2014): Effects of collagen tripeptide supplement on skin properties: A prospective, randomized, controlled study. Journal of Cosmetic and Laser Therapy 16: 132-137.

Kao, H.-Y., Jen, S.-I., and Kao, Y.-C. (2022): Evaluation of the anti-aging and whitening efficacy of collagen tripeptide and chicken protein hydrolysate. Journal of Applied Biological Sciences 16: 447-455.

Blois, M. S. (1958): Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.

Rahman, M. M., Islam, M. B., Biswas, M., and Khurshid Alam, A. H. M. (2015): In vitro antioxidant and free radical scavenging activity of different parts of tabebuia pallida growing in bangladesh. BMC Research Notes 8: 621.

Suarez-Arnedo, A., Torres Figueroa, F., Clavijo, C., Arbeláez, P., Cruz, J. C., and Muñoz-Camargo, C. (2020): An image j plugin for the high throughput image analysis of in vitro scratch wound healing assays. PLOS ONE 15: e0232565.

Au - Kandárová, H., Au - Hayden, P., Au - Klausner, M., Au - Kubilus, J., and Au - Sheasgreen, J. (2009): An in vitro skin irritation test (sit) using the epiderm reconstructed human epidermal (rhe) model. JoVE e1366.

OECD, Test no. 439: In vitro skin irritation: Reconstructed human epidermis test method, 2021.

Comino-Sanz, I. M., López-Franco, M. D., Castro, B., and Pancorbo-Hidalgo, P. L. (2021): The role of antioxidants on wound healing: A review of the current evidence. J Clin Med 10(16): 3558.

Freiesleben, S. H., Soelberg, J., Nyberg, N. T., and Jäger, A. K. (2017): Determination of the wound healing potentials of medicinal plants historically used in ghana. Evidence-Based Complementary and Alternative Medicine 2017: 9480791.

Ghorbanpour Arani, A., Miralaei, N., Farazin, A., and Mohammadimehr, M. (2023): An extensive review of the repair behavior of smart self-healing polymer matrix composites. Journal of Materials Research 38: 617-632.

Farazin, A., Mohammadimehr, M., and Naeimi, H. (2023): Flexible self-healing nanocompositebased gelatin/tannic acid/acrylic acid reinforced with zinc oxide nanoparticles and hollow silver nanoparticles based on porous silica for rapid wound healing. International Journal of Biological Macromolecules 241: 124572.




How to Cite

Kao, H.-Y. ., Jen, S.-I. ., Kao, Y.-C. ., Kuo, Z.-K., & Liu, S.-H. . (2023). ANTIOXIDANT ACTIVITIES AND IN VITRO WOUND HEALING EFFECTS OF META COLLAGEN. Journal of Applied Biological Sciences, 17(3), 500–509. Retrieved from