INVESTIGATION OF CHOLESTEROL AND BLOOD GLUCOSE-REDUCING PROPERTIES AND ACUTE TOXICITIES OF THE TEA PREPARED BY VERNONIA CINEREA L. USING WISTAR RAT ANIMAL MODEL AND QUANTIFICATION OF FLAVONOID AND PHENOLIC CONTENTSAbstract views: 41 / PDF downloads: 99
Keywords:V. cinerea, C. sinensis, phytochemicals, wistar rats, health benefits
According to Ayurvedic and folkloric medicine of Sri Lanka, tea prepared using dried leaf powder of Vernonia cinerea L. (S. Monarakudummbiya) has many therapeutic effects including blood glucose and cholesterol-reducing properties. The study investigate cholesterol and blood glucose-reducing properties and acute toxicities exerted by V. cinerea L tea and quantify the total phenolic and flavonoid content. Three months Wistar (20) rats were recruited for the study and divided randomly (n=10) into two groups. One group of rats was daily given tea prepared using the leaf powder of V. cinerea while the other group was given an equal volume of distilled water. Their baseline biochemical parameters, such as blood glucose, total cholesterol, high-density lipoprotein (HDL-cholesterol), triglycerides, creatinine levels, alanine transaminase (ALT), aspartate transaminase (AST) activities were measured. This experiment was carried out for 28 continuous days and repeated the same biochemical parameters on the 29th day. In addition, total phenolic and flavonoid contents were evaluated in the V. cinerea tea. There was a significant increment of HDL cholesterol levels whilst total cholesterol, triglycerides and blood glucose remained unaffected. There was no significant increase in ALT, AST and creatinine, indicating no acute toxicities in the prepared tea. Total phenolic and total flavonoid content in V. cinerea tea were GAE/g of extract at 28.62±1.06 and 19.20±2.7 respectively. V. cinerea tea was rich in therapeutically important phytochemicals such as phenols and flavonoids that can significantly increase HDL cholesterol levels in Wistar rats. V. cinerea can be further improved as a green tea.
Digwal, M., Tarekar, D., Surji, A., Amravati, D. (2017): Pharmacognostic studies of Vernonia cinerea (L.) Leaves - an antismoking plant. Pharmaceut Res 6 (1), pp. 875-882.
Lakshmi Prabha, J. (2015): Therapeutic uses of Vernonia cinerea. A short review. International Journal of Pharmaceutical and Clinical Research 7(4): 323-325.
Haque, M.A., Hassan, M.M., Das, A., Begum, B., Ali, M.Y., Horshed, H. (2012): Phytochemical investigations of Vernonia cinerea (Family: Asteraceae). Journal of Applied Pharmaceutical Science 2(6): 78-83. https://doi.org/10.7324/JAPS.2012.2617.
Iwalewa, E.O., Iwalewa, O.J., Adeboye, J.O. (2003): Analgesic, antipyretic, anti-inflammatory effects of methanol, chloroform and ether extracts of Vernonia cinerea Less leaf. Journal of Ethnopharmacology 86(2-3): 229-234. https://doi.org/10.1016/S0378-8741(03)00081-3.
Gupta, M., Mazumder, U.K., Manikandan, L., Haldar, P.K., Bhattacharya, S., Kandar, C.C. (2003): Antibacterial activity of Vernonia cinerea. Fitoterapia 74(1-2):148-150. https://doi.org/10.1016/s0367-326x(02)00291-5.
Kumar, P.P., Kuttan, G. (2009): Vernonia cinerea L. scavenges free radicals and regulates nitric oxide and proinflammatory cytokines profile in carrageenan-induced paw edema model. Immunopharmacology and Immunotoxicology 31(1):94-102. https://doi.org/10.1080/08923970802438391.
Daffodil, E.D., Lincy, P., Mohan, V.R. (2014): Study of the whole plant of Vernonia cinerea Less for in vitro antioxidant activity. International Journal of Pharmacy 4(2): 172-178.
Sonibare, M.A., Aremu, O.T., Okorie, P.N. (2016): Antioxidant and antimicrobial activities of solvent fractions of Vernonia cinerea (L.) Less leaf extract. African Health Sciences 16(2):629-39. https://doi.org/10.4314/ahs.v16i2.34.
Pakpisutkul, J., Suwapraphan, J., Sripayak, N., Sitkhuntod, N., Loyrat, S., Yahayo, W., Supabphol, R. (2022): The effects of Vernonia cinerea Less extracts on antioxidant gene expression in colorectal cancer cells. Asian Pacific Journal of Cancer Prevention 23(11), 3923 – 3930. https://doi.org/10.31557/APJCP.2022.23.
Marcus, D.M., Grollman, A.P. (2016): Toxicity of Botanical Medicines: An Overlooked Global Health Problem. American Journal of Public Health 106(1):16-7. https://doi.org/ 10.2105/AJPH.2015.302937.
Charan, J., Biswas, T. (2013): How to calculate sample size for different study designs in medical research. Indian Journal of Psychological Medicine 35(2):121-126. https://doi.org/10.4103/0253-7176.116232.
Wariyapperuma, W.A.N.M., Jayawardena, B.M., Kannangara, S.D.P., Wijayasinghe, Y.S., Subramaniam, S.S., Karunakaran, R., Kumara, W.G.S.S., Thammitiyagodage, M.G. (2019): Blood sample collection procedure and biochemical parameters of 6-8 weeks old male and female Wistar rats under Isoflurane anesthesia. Proceeding of Annual Scientific Sessions of the Sri Lanka Association for Laboratory Animal Science.
Singleton, V. L., Orthofer, R., Lamuela-Raventós, R.M. (1999): Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology 299: 152-178. http://dx.doi.org/10.1016/S0076-6879(99)99017-1.
Meda, A., Lamien, C. E., Romito, M., Millogo, J., Nacoulma, O.G. (2005): Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry 91(3): 571-577. https://doi.org/10.1016/j.foodchem.2004.10.006.
Musunuru, K. (2010): Atherogenic dyslipidemia: Cardiovascular risk and dietary intervention. Lipids 45(10): 907-914. https://doi.org/10.1007/s11745-010-3408-1.
Moore, K.J., Tabas, I. (2011): Macrophages in the pathogenesis of atherosclerosis. Cell 145(3):341– 355. https://doi.org/10.1016/j.cell.2011.04.005.
Saboudry, M.A. (1988): Breeding and Care of Laboratory Animals. Volume 1, pp18-19.WHO. Health laboratory Technology Unit. Geneva. Switzerland.
Samaranayake, H.A.E., Chackrewarthy, S., Wickremasinghe, A.R., Thammitiyagodage, M.G., Karunakaran, R. (2015): Determination of the proximate composition of rat feed used at the Medical Research Institute (MRI), Sri Lanka. Proceedings of the 2nd Scientific Session of the Sri Lanka Association for Laboratory Animal Science.
Messina, M., Shearer, G., Petersen, K. (2021): Soybean oil lowers circulating cholesterol levels and coronary heart disease risk and has no effect on markers of inflammation and oxidation. Nutrition (89), 2021, 111343.
Arawwawala, L.D.A.M., Thabrew, I., Arambewela, L.S.R. (2011): Evaluation of toxic potential of standardized aqueous and ethanolic extracts of Trichosanthes cucumerina in rats. Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants (BLACPMA) 1, 11-22.
Castro-Barquero, S., Tresserra-Rimbau, A., Vitelli-Storelli, F., Doménech, M., Salas-Salvadó, J., Martín-Sánchez, V., Rubín-García, M., Buil-Cosiales, P., Corella, D., Fitó, M… et al. (2020): Dietary Polyphenol Intake is Associated with HDL-Cholesterol and A Better Profile of other Components of the Metabolic Syndrome: A PREDIMED-Plus Sub-Study. Nutrients.; 12(3):689.
Abeywardena, K.K., Thammitiyagodage, M.G., Kumara, W.G.S.S., Munasinghe, A.T.M., Arawwawala, L.D.A.M (2023): Phenol and Flavonoid Contents of Vernonia cinerea (L.) and Low-Grown Sri Lankan Green Tea Camelia sinensis. Proceeding of the 10th Annual Scientific Sessions and International Conference of the Sri Lanka Association for the Laboratory Animal Science, Colombo, Sri Lanka.
Ciumărnean, L., Milaciu, M.V., Runcan, O., Vesa, Ș.C., Răchișan, A.L., Negrean, V., Perné, M.G., Donca, V.I., Alexescu, T.G., Para, I., Dogaru, G. (2020): The Effects of Flavonoids in Cardiovascular Diseases. Molecules 21;25(18):4320.
Rahman, M. M., Rahaman, M.S., Islam, M.R., Rahman, F., Mithi, F.M., Alqahtani, T., Almikhlafi, M.A., Alghamdi, S.Q., Alruwaili, A.S., Hossain, M.S., Ahmed, M., Das, R., Emran, T.B, Uddin, M.S (2021): Role of Phenolic Compounds in Human Disease: Current Knowledge and Future Prospects. Molecules 30;27(1):233.
Ferguson, M.A., Waikar, S.S (2012): Established and emerging markers of kidney function. Clin Chem. 58(4):680-689.
Wang, C., Han, J., Pu, Y., Wang, X. (2022): Tea (Camellia sinensis): A Review of Nutritional Composition, Potential Applications, and Omics Research. Applied Sciences 12(12):5874. https://doi.org/10.3390/app12125874.
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