ISOLATION OF DNA BY PHENOL-CHLOROFORM EXTRACTION METHOD FROM SHED AND WAITING FEATHERS OF PSITTACIFORMES BIRD ORDER
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Bird, DNA isolation, feather, psittaciformesAbstract
In this study, it was aimed to isolate DNA from shed and waiting (at least two weeks) wing/tail feathers of the Psittaciformes bird order (Ara chloropterus, Cacatua galerita, Lorius garrulous, Amazona ochrocephala, Ara ararauna, Psittacus erithacus, n=17) using the phenol-chloroform-isoamyl alcohol extraction method. The isolated DNAs was subjected to polymerase chain reaction with a primer commonly used for sex determination in birds. According to the nanodrop measurement results, it was revealed that sufficient DNA concentration to be used in DNA-based molecular studies can be obtained using the phenol-chloroform isolation method. Gender-specific band images were obtained from all DNA samples isolated according to PCR analysis performed for control purposes. As a result, it was revealed that DNA isolation can be done successfully with the Phenol-chloroform isolation method from the shed and waiting wing/tail feathers of the bird species Psittaciformes. It was concluded that non-invasively obtained DNAs can be used in molecular methods.
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