GENETIC DIVERSITY ANALYSIS OF CULTIVATED AND WILD MANGOES FROM BANGLADESH BASED ON PCR AMPLIFICATION PATTERN OF TANDEMLY ORGANIZED REPEAT SEQUENCES
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Mangifera indica, RepeatExplorer2, repetitive DNA, satellite DNAAbstract
Mangifera is an economically important genus mainly because of the delicious fruit produced by many species of this genus. Wild species available in Bangladesh of this genus are often misidentified and have ambiguous taxonomic leveling, due to the difficulty of getting the appropriate sample for plant identification. Tandem repeat diversity was analyzed from the Mangifera genome, to understand their impact on the genomic diversity of different species and cultivars available in Bangladesh. We use tandem repeats amplification pattern for mango diversity analysis because of the fact that satellite repeats (longer arrayed tandem repeats) are usually rapidly evolving parts of the genome, found to even diversify in closely related species, hence is useful to study genome diversity analysis. RepeatExplorer2-based bioinformatics analysis was performed to identify tandemly organized repeat sequences. Face to face PCR primers were developed from each of the consensus sequences of the identified tandem repeats sequences. Our results showed that the studied samples showed a tandem repeat-specific PCR amplification pattern that corresponds to their monomer size variation, confirming the presence of genomic organization of each repeat type. Three primer pairs targeting three tandem repeat specific clusters CL18, CL165 and CL335 showed genotype specific patterns, while CL201 and CL283 produced similar banding patterns in all the studied genotype. However, CL19 failed to follow any consistent banding pattern in all three species and cultivars except smear. Our results suggest that although cultivated M. indica genotypes are very similar in terms of tandem repeat diversity but the wild species collected from different locations of Bangladesh are indeed different. Therefore, in depth taxonomic investigation is necessary to solve the taxonomic ambiguity of wild Mangifera germplasm available in Bangladesh.
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