GREEN SYNTHESIS OF SILVER NANOPARTICLES USING ANDROGRAPHIS PANICULATA LEAVES EXTRACT AND THEIR ANTIPROLIFERATIVE ACTIVITY IN HUMAN LUNG ADENOCARCINOMA CELLS

Abstract views: 36 / PDF downloads: 93

Authors

Keywords:

Phytochemicals, nanoparticles, oxidative stress, cell viability assay, green method

Abstract

Cancer is emerging as a global pandemic affecting millions of people. It is often characterised by uncontrolled cell growth due to loss in death mechanism. Conventional anti-cancerous drugs and therapies exhibits other life-threatening side effects. Thus, design and development of new anti-cancer therapeutic agents is the need of time. Metal nanoparticles synthesis via green method have been studying prodigiously worldwide for safe and efficient anti-cancer agents. However, to impart effects of nanoparticles their persistent with the cells are important and for this synthesis of nanoparticles with proper shape and size is important. This study has been focused on the silver nanoparticles synthesis using Andrographis paniculate plant leaves aqueous extract and their evaluation for anti-lung cancer activity. Green method synthesised metal nanoparticles were characterised by Scanning Electron Microscopy (SEM), Energy dispersive X Ray analysis (EDAX), Transmission Electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy analysis. FTIR analysis showed presence of varied phytochemical components. SEM analysis of the sample elucidated rough spherical morphological characteristics of the material, while elemental analysis through EDAX technique reported silver (Ag) metal in synthesised sample. TEM analysis confirmed nanoparticles size ranging from 18-52 nm. Result of the study showed decreased cell viability of A549 cells treated over green method synthesised silver nanoparticles.

References

An, K., & Somorjai, G. A. (2012): Size and shape control of metal nanoparticles for reaction selectivity in catalysis. ChemCatChem 4(10): 1512-1524.

Morachis, J. M., Mahmoud, E. A., & Almutairi, A. (2012): Physical and chemical strategies for therapeutic delivery by using polymeric nanoparticles. Pharmacological Reviews 64(3): 505-519.

Iravani, S., Korbekandi, H., Mirmohammadi, S. V., & Zolfaghari, B. (2014): Synthesis of silver nanoparticles: chemical, physical and biological methods. Research in Pharmaceutical Sciences 9(6): 385.

Sevindik, M., Mohammed, F. S., & Uysal, I. (2023): Autism: plants with neuro-psychopharmacotherapeutic potential. Prospects in Pharmaceutical Sciences, 21(3): 38-48.

Acamovic, T., & Brooker, J. D. (2005): Biochemistry of plant secondary metabolites and their effects in animals. Proceedings of the Nutrition Society 64(3): 403-412.

Jabeen, S., Qureshi, R., Munazir, M., Maqsood, M., Munir, M., Shah, S. S. H., & Rahim, B. Z. (2021): Application of green synthesized silver nanoparticles in cancer treatment—a critical review. Materials Research Express 8(9): 092001.

Singh, J., Singh, T., & Rawat, M. (2017): Green synthesis of silver nanoparticles via various plant extracts for anti-cancer applications. Nanomedicine 7(3): 1-4.

Gomathi, A. C., Rajarathinam, S. X., Sadiq, A. M., & Rajeshkumar, S. (2020): Anticancer activity of silver nanoparticles synthesized using aqueous fruit shell extract of Tamarindus indica on MCF-7 human breast cancer cell line. Journal of Drug Delivery Science and Technology 55: 101376.

Siegel, R. L., Miller, K. D., Wagle, N. S., & Jemal, A. (2023): Cancer statistics, 2023. CA: A Cancer Journal for Clinicians 73(1): 17–48.

Sathishkumar, K., Chaturvedi, M., Das, P., Stephen, S., & Mathur, P. (2022): Cancer incidence estimates for 2022 & projection for 2025: Result from National Cancer Registry Programme, India. The Indian Journal of Medical Research 156(4&5): 598–607.

Singh, N., Agrawal, S., Jiwnani, S., Khosla, D., Malik, P. S., Mohan, A., Penumadu, P., & Prasad, K. T. (2021): Lung Cancer in India. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 16(8): 1250–1266.

Chao, W. W., & Lin, B. F. (2010): Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian). Chinese Medicine 5: 1-15.

Akbar, S. (2011): Andrographis paniculata: a review of pharmacological activities and clinical effects. Alternative Medicine Review 16(1): 66-77.

Dongre, U., Meshram, T., Dighe, S., Narnawre, K., Mehere, B., & Somkuwar, S. R. (2020): Screening of selected ethno-medicinal plants for anti-cancer activity. Adv Zool Bot 8(5): 447-452.

Kotakadi, V. S., Gaddam, S. A., Rao, Y. S., Prasad, T. N. V. K. V., Reddy, A. V., & Gopal, D. S. (2014): Biofabrication of silver nanoparticles using Andrographis paniculata. European Journal of Medicinal Chemistry 73: 135-140.

Sinha, S. N., & Paul, D. (2015): Phytosynthesis of silver nanoparticles using Andrographis paniculata leaf extract and evaluation of their antibacterial activities. Spectroscopy Letters 48(8): 600-604.

Fardiyah, Q., Ersam, T., Slamet, A., & Kurniawan, F. (2020): New potential and characterization of Andrographis paniculata L. Ness plant extracts as photoprotective agent. Arabian Journal of Chemistry 13(12): 8888-8897.

Raghunandan, D., Ravishankar, B., Sharanbasava, G., Mahesh, D. B., Harsoor, V., Yalagatti, M. S., ... & Venkataraman, A. (2011): Anti-cancer studies of noble metal nanoparticles synthesized using different plant extracts. Cancer Nanotechnology 2: 57-65.

Mohammadzadeh, V., Barani, M., Amiri, M. S., Yazdi, M. E. T., Hassanisaadi, M., Rahdar, A., & Varma, R. S. (2022): Applications of plant-based nanoparticles in nanomedicine: A review. Sustainable Chemistry and Pharmacy 25: 100606.

Khattak, K. F. (2015): Effect of geographical distributions on the nutrient composition, phytochemical profile and antioxidant activity of Morus nigra. Pakistan Journal of Pharmaceutical Sciences 28(5).

Korkmaz, A. İ., Bal, C., Eraslan, E. C., Sevindik, M., & Akgul, H. (2023): Biological activities of Agrocybe praecox (spring fieldcap mushroom). Prospects in Pharmaceutical Sciences 21(4): 33-39.

Botcha, S., & Prattipati, S. D. (2020): Callus extract mediated green synthesis of silver nanoparticles, their characterization and cytotoxicity evaluation against MDA-MB-231 and PC-3 cells. BioNanoScience 10: 11-22.

Mundekkad, D., & Cho, W. C. (2022): Nanoparticles in clinical translation for cancer therapy. International Journal of Molecular Sciences 23(3): 1685.

González-Ballesteros, N., Diego-González, L., Lastra-Valdor, M., Rodríguez-Argüelles, M. C., Grimaldi, M., Cavazza, A., ... & Simón-Vázquez, R. (2019): Immunostimulant and biocompatible gold and silver nanoparticles synthesized using the Ulva intestinalis L. aqueous extract. Journal of Materials Chemistry B 7(30): 4677-4691.

Piao, M. J., Kang, K. A., Lee, I. K., Kim, H. S., Kim, S., Choi, J. Y., ... & Hyun, J. W. (2011): Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicology Letters 201(1): 92-100.

Guntupalli, Y., Raghunandhakumar, S., Ezhilarasan, D., & Lakshmi, T. (2022): Anti proliferative activity of andrographis paniculata whole plant extract on a549 lung cancer cell line. International Journal of Early Childhood Special Education 14(5).

Downloads

Published

2024-02-06

How to Cite

Gajbhiye, S. ., Pal, K., & Dongre, U. (2024). GREEN SYNTHESIS OF SILVER NANOPARTICLES USING ANDROGRAPHIS PANICULATA LEAVES EXTRACT AND THEIR ANTIPROLIFERATIVE ACTIVITY IN HUMAN LUNG ADENOCARCINOMA CELLS. Journal of Applied Biological Sciences, 18(1), 118–126. Retrieved from https://jabsonline.org/index.php/jabs/article/view/1254

Issue

Section

Articles