GREEN SYNTHESIS OF SILVER NANOPARTICLES USING ANDROGRAPHIS PANICULATA LEAVES EXTRACT AND THEIR ANTIPROLIFERATIVE ACTIVITY IN HUMAN LUNG ADENOCARCINOMA CELLS
DOI:
https://doi.org/10.71336/jabs.1254Keywords:
Phytochemicals, nanoparticles, oxidative stress, cell viability assay, green methodAbstract
Cancer is emerging as a global pandemic affecting millions of people. It is often characterised by uncontrolled cell growth due to loss in death mechanism. Conventional anti-cancerous drugs and therapies exhibits other life-threatening side effects. Thus, design and development of new anti-cancer therapeutic agents is the need of time. Metal nanoparticles synthesis via green method have been studying prodigiously worldwide for safe and efficient anti-cancer agents. However, to impart effects of nanoparticles their persistent with the cells are important and for this synthesis of nanoparticles with proper shape and size is important. This study has been focused on the silver nanoparticles synthesis using Andrographis paniculate plant leaves aqueous extract and their evaluation for anti-lung cancer activity. Green method synthesised metal nanoparticles were characterised by Scanning Electron Microscopy (SEM), Energy dispersive X Ray analysis (EDAX), Transmission Electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy analysis. FTIR analysis showed presence of varied phytochemical components. SEM analysis of the sample elucidated rough spherical morphological characteristics of the material, while elemental analysis through EDAX technique reported silver (Ag) metal in synthesised sample. TEM analysis confirmed nanoparticles size ranging from 18-52 nm. Result of the study showed decreased cell viability of A549 cells treated over green method synthesised silver nanoparticles.
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