ANTIFUNGAL ACTIVITY OF GARLIC EXTRACT ON FUNGI ASSOCIATED WITH URINARY TRACT INFECTIONS AMONG FEMALE STUDENTS OF JOSEPH SARWUAN TARKA UNIVERSITY
DOI:
https://doi.org/10.71336/jabs.1257Keywords:
Antifungal activity, garlic extract, isolates, urinary tract infectionsAbstract
Researchers from all around the world are becoming more interested in the medicinal properties of plant extracts. Since modern medicine has its own benefits and drawbacks, plant-based solutions are becoming more and more popular since they are risk-free, readily available, and reasonably priced. The main purpose of this study was to determine the antifungal activity of garlic (Allium sativum) extract on fungi associated with urinary tract infections among female students of Federal University of Agriculture Makurdi. Garlic cloves were purchased from North Bank market and were transported to Microbiology Laboratory. They were blended into paste and were extracted using maceration method. Urine samples collected were analyzed using standard microbiological methods. Extract concentrations from 500mg/mL-100mg/mL were prepared using a double standard dilution method. About 50 urine samples were collected and the fungi present in the urine were isolated using standard microbiological methods. Susceptibility analysis of the fungal isolates was done using the aqueous and ethanolic garlic extract, and the zones of inhibitions obtained at the various concentrations were compared with a positive control (fluconazole). Analysis of data obtained was done using a Statistical Package for Social Science (SPSS). The result showed a mean zone of inhibition of 17.6×10± 1.00×10 mm at a concentration of 500mg/mL, 13.6×10 ± 1.52×10 mm at a concentration of 250mg/mL and 22.0×10 ± 1.00x10mm for the positive control (fluconazole) on Candida albicans using the aqueous garlic extract while for the ethanolic garlic extract showed a mean zone of inhibition of 18.3x10±1.52×10mm at a concentration of 500mg/mL 13.0×10 ± 2.00×10 mm at 250mg/mL. The mean zones of inhibition for 200mg/mL, 125mg/mL, and 100mg/mL were 9.67x10±5.77×10 mm, 7.67×10 ±5.77×10 mm and 3.67×10± 5.77×10mm respectively. Garlic extract has potential antifungal activity on the isolated urinary tract organisms and as such can be used as a natural antifungal agent in the control of fungal urinary tract infections.
References
Moyo, B., Masika, P. J., Muchenje, V. (2012): Antimicrobial activities of Moringa oleifera Lam leaf extracts. African Journal of Biotechnology, 11(11): 2797-2802. DOI: https://doi.org/10.5897/AJB10.686
Carvalho, D. D. C., Alves, E., Camargos, R. B., Oliveira, D. F., Scolforo, J. R. S., de Carvalho, D. A., Batista, T. R. S. (2011): Plant extracts to control Alternaria alternata in Murcott tangor fruits. Revista Iberoamericana de Micología, 28(4): 173-178. DOI: https://doi.org/10.1016/j.riam.2011.05.001
Combrinck, S., Regnier, T., Kamatou, G. P. (2011): In vitro activity of eighteen essential oils and some major components against common postharvest fungal pathogens of fruit. Industrial Crops and Products, 33(2): 344-349. DOI: https://doi.org/10.1016/j.indcrop.2010.11.011
Tsai, C. W., Chen, H. W., Sheen, L. Y., Lii, C. K. (2012): Garlic: Health benefits and actions. BioMedicine, 2(1): 17-29. DOI: https://doi.org/10.1016/j.biomed.2011.12.002
Dayan, F. E., Cantrell, C. L., Duke, S. O. (2009): Natural products in crop protection. Bioorganic & medicinal chemistry, 17(12): 4022-4034. DOI: https://doi.org/10.1016/j.bmc.2009.01.046
Du Plooy, W., Regnier, T., Combrinck, S. (2009): Essential oil amended coatings as alternatives to synthetic fungicides in citrus postharvest management. Postharvest Biology and Technology, 53(3): 117-122. DOI: https://doi.org/10.1016/j.postharvbio.2009.04.005
Bakri, I. M., Douglas, C. W. I. (2005): Inhibitory effect of garlic extract on oral bacteria. Archives of oral biology, 50(7): 645-651. DOI: https://doi.org/10.1016/j.archoralbio.2004.12.002
Shams-Ghahfarokhi, M., Shokoohamiri, M. R., Amirrajab, N., Moghadasi, B., Ghajari, A., Zeini, F., Sadeghi, G., Razzaghi-Abyaneh, M. (2006): In vitro antifungal activities of Allium cepa, Allium sativum and ketoconazole against some pathogenic yeasts and dermatophytes. Fitoterapia, 77(4): 321-323. DOI: https://doi.org/10.1016/j.fitote.2006.03.014
Sabitha, P., Adhikari, P. M., Shenoy, S. M., Kamath, A., John, R., Prabhu, M. V., Padmaja, U. (2005): Efficacy of garlic paste in oral candidiasis. Tropical doctor, 35(2): 99-100. DOI: https://doi.org/10.1258/0049475054037084
Sk, B. (2002): Effect of garlic on cardiovascular disorders: a review. Nutr J., 1: 4-14. DOI: https://doi.org/10.1186/1475-2891-1-4
Josling, P. (2001): Preventing the common cold with a garlic supplement: a double-blind, placebo-controlled survey. Advances in therapy, 18, 189-193. DOI: https://doi.org/10.1007/BF02850113
Jung, Y. M., Lee, S. H., Lee, D. S., You, M. J., Chung, I. K., Cheon, W. H., Kwon, Y.S., Lee, Y.J., Ku, S. K. (2011): Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects. Nutrition research, 31(5): 387-396. DOI: https://doi.org/10.1016/j.nutres.2011.04.005
Okigbo, R. N., Okorie, R. E., Putheti, R. R. (2009): In vitro effects of garlic (Allium sativum L.) and African basil (Ocimum gratissimum L.) on pathogens isolated from rotted cassava roots. Interciencia, 34(10): 742-747.
Lemar, K. M., Aon, M. A., Cortassa, S., O'Rourke, B., Müller, C. T., Lloyd, D. (2007): Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress‐mediated cell death studied by two‐photon microscopy. Yeast, 24(8): 695-706. DOI: https://doi.org/10.1002/yea.1503
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