ANTIBACTERIAL ACTIVITY OF LEUCONOSTOC MESENTEROIDES ISOLATED FROM RAW GOAT MILK
DOI:
https://doi.org/10.71336/jabs.1261Keywords:
Antagonistic, bio-preservation, fermentations, leuconostoc, MALDI-TOFAbstract
Leuconostoc are widespread in the natural environment and play an important role in several fermentations due to their physiological characteristics. With the aim of studying the biological potential of these bacteria four samples of goat raw milk were collected from north-western Algeria and used as sources of isolation of Leuconostoc. A total of 12 isolates of Leuconostoc mesenteroides were obtained by sequential screening for morphological, chemically and MALDI-TOF identification tests. All isolates were observed to behave antagonistically with all indicator strains, with differences in the size of the inhibition zone by the stain diffusion method. Contrariwise to the results of the diffusion method, only seven strains of these isolates were able to inhibit Gram-positive strains with variable resistance according to each strain after heat resistance tests (60°C to 120°C) and acidity (pH 2 to 9) with complete disappearance of this inhibitory activity when their supernatant was treated with pepsin and α-chymotrypsin. The strain W9 was selected as the most efficient to study its capacity of bio-preservation food by the follow-up of the kinetics of pH, the production of lactic acid and the growth in pure culture and mixed with strains of spoilages and/or pathogens. The results obtained showed that these isolates have important antagonistic and conservative potential. It could be concluded that they could be effective candidates for a local application of bio-conservation.
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