INVESTIGATION OF ESβL, AMP-C, CARBAPENEMASE PRODUCING ESCHERICHIA COLI STRAINS ANTIMICROBIAL RESISTANCE PROPERTIES ISOLATED FROM RED MEAT IN RETAIL BY MIC, PCR, AND LAMP PCR ANALYSIS

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Keywords:

Escherichia coli, PCR, ESβL, Amp-C, carbapenemase

Abstract

In this study; phenotypic antibiotic resistance properties of ESβL, Amp-C, and carbapenemase producing Escherichia coli strains isolated from red meats offered for retail sale were analyzed by E-Test and disc diffusion test. Antibiotic resistance genes were determined by PCR and LAMP PCR methods. Twelve E. coli strains were isolated from 120 red meat samples that were collected and it was verified by a rapid identification system (BBL Crystal). In 10 isolates, as a result of the disk diffusion test of the isolates, ESβL activity was determined; resistance was seen to ampicillin and tetracycline with 91.6%, cefotaxime with 83.33%, chloramphenicol, ciprofloxacin and nalidixic acid with 75%, azithromycin, and sulfamethoxazole/trimethoprim with 66%, gentamicin, and ceftazidime with 41.6%, and tigecycline with 33.3%; no resistance to meropenem was observed. In addition, all isolates were resistant to cefoxitin. With the E-test, ESβL activity was found in 1 isolate; Amp-C and carbapenemase activity were not detected in any of the isolates. As a result of PCR, when examined in terms of ESβL activity, in 4 isolates, the CTX-M1 gene was detected, and the SHV gene was determined in all E. coli isolates; Amp-C and carbapenemase genes were not detected. When carbapenemase genes were examined by LAMP PCR, two isolates were positive and all of the 6 carbapenemase genes that were examined were found in these two positive isolates. Our study, it was aimed to determine the resistance genes of red meats within 24 hours of pre-enrichment without agent isolation, and the same results were obtained, in addition. Detection of these resistance genes in retail red meat poses a risk to public health. In line with the concept of one health, it is important for public health to detect the resistance genes detected in red meat and take the necessary measures to reduce this resistance.

References

Butler, C.C., Hıllıer, S., Roberts, Z., Dunstan, F., Howard, A., Palmer, S.R. (2006): Antibiotic-resistant infections in primary care are symptomatic for longer and increase workload: outcomes for patients with E. coli UTIs. Br J Gen Pract 56: 686-92.

Nadeem, S.F., Gohar, U.F., Tahir, S.F., Mukhtar, H., Pornpukdeewattana, S., Nukthamna, P, Ali, A.MM., Bavisetty, S.C.B., Massa, S. (2020): Antimicrobial resistance: more than 70 years of war between humans and bacteria. Crit Rev Microbiol 46 (5): 578-599. https://doi.org/10.1080/1040841x.2020.1813687.

European Food Safety Authority (EFSA). (2011): Scientific opinion on the public health risks of bacterial strains producing extendedspectrum β-lactamases and/or AmpC β-lactamases in food and foodproducing animals. EFSA Journal 9: 2322–2417. https://doi.org/10.2903/j.efsa.2011.2322.

WHO. (2013): Integrated surveillance of antimicrobial resistance: guidance from a WHO Advisory Group. ISBN 978 92 4 150631 1, Switzerland, 2013.

Silva, N., Carvalho, I., Currie, C., Sousa, M., Igrejas, G., Poeta, P. (2019): Extended-spectrum-β-lactamase and carbapenemase-producing Enterobacteriaceae in food-producing animals in Europe: An Impact on Public Health? In, Capela-Martinez JL, Igrejas G. (editor). Antibiotic Drug Resistance, Chapter 12. 261–273, Wiley Online Library, 2019. pp.

Ramos. S,, Silva, V., Dapkevicius, M.D.L.E., Caniça, M., Tejedor-Junco, M.T., Teresa, M. (2020): Escherichia coli as commensal and pathogenic bacteria among food-producing animals: Health implications of extended spectrum β-lactamase (ESBL) production. Animals (Basel) 10(12): 2239. https://doi.org/10.3390/ani10122239.

Mughini-Gras, L., Dorado-García, A., van Duijkeren, E., van den Bunt, G., Dierikx, C.M., Bonten, M.J.M., Bootsma, M.C.J., Schmitt, H., Hald, T., Evers, E.G., Koeijer, A.D, Pelt, W.V., Franz, E., Mevius, D.J., Heederik, D.J.J. (2019): Attributable sources of community-acquired carriage of Escherichia coli containing β-lactam antibiotic resistance genes: A population-based modelling study. Lancet. Planetary Health 3: e357–e369. https://doi.org/10.1016/S2542-5196(19)30130-5.

Fadare, F.T., Adefisoye, M.A., Okoh, A.I. (2020): Occurrence, identification, and antibiogram signatures of selected Enterobacteriaceae from Tsomo and Tyhume rivers in the Eastern Cape Province, Republic of South Africa. Plos one 15(12): e0238084.

Thomson, K.S. (2010): Extended-spectrum-β-lactamase, AmpC, and Carbapenemase issues. J Clin Microbiol 48: 1019–1025.

Poirel, L., Lebessi, E., Castro, M., Fèvre, C., Foustoukou, M., Nordmann, P. (2004): Nosocomial outbreak of extended-spectrum β-lactamase SHV-5-producing isolates of Pseudomonas aeruginosa in Athens, Greece. Antimicrob Agent Chemothe 48(6): 2277-2279. https://doi.org/10.1128/AAC.48.6.2277-2279.2004

Köck, R., Daniels-Haardt, I., Becker, K., Mellmann, A., Friedrich, A.W., Mevius, D., Schwarz, S., Jurke, A. (2018): Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review. Clin Microbiol Infect. Epub ahead of print https://doi.org/10.1016 /j.cmi.2018.04.004.

Woodford, N., Wareham, D.W., Guerra, B., Teale, C. (2014): Carbapenemaseproducing Enterobacteriaceae and non-Enterobacteriaceae from animals and the environment: an emerging public health risk of our own making? J Antimicrob Chemother 69: 287–291.

Day, M.J., Hopkins, K.L., Wareham, D.W., Toleman, M.A., Elviss, N., Randall, L., Teale, C., Cleary, P., Wiuff, C., Doumith, M., Ellington, M.J., Woodford, N., Livermore, D.M. (2019): Extended-spectrum β-lactamase-producing Escherichia coli in human-derived and food chain-derived samples from England, Wales, and Scotland: An epidemiological surveillance and typing study. Lancet Infect Dis 2019; 19: 1325–1335. https://doi.org/10.1016/S1473-3099(19)30273-7.

European Union Reference Laboratory-Antimicrobial Resistance, Laboratory Protocol. (2019): Isolation of ESBL-, AmpC- and carbapenease producing E. coli from fresh meat. Version 7

Dallenne, C., Costa, A., Decre, D., Favier, C., Arlet, G. (2010): Development of a set of multiplex PCR assays for the detection of genes encoding important b-lactamases in Enterobacteriaceae J Antimicrob Chemother 65: 490–495. https://doi.org/10.1093/jac/dkp498.

Lahiri, S., Venkataraman, R., Jagan, A., Deshmukh, G., Patra, S., Reddy, V., Sangeetha, V., Solanki, R., Gupta, J., Patel, K., De, A., Mukhopadhyay, C., Dias, M., Kanungo, R., Mendiratti, D., Nawal, P., Shastri, J., Vemu, L., Rangarajan, R. (2019): Evaluation of LAMP-based assays for carbapenemase genes. J Med Microbiol 68(10): 1431-1437. https://doi.org/10.1099/jmm.0.001050.

European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2021): MIC and zone diameter distributions and ECOFFs 2021.

Clinical and Laboratory Standards Institute. (2014): Performance standarts for antimicrobial susceptibility testing; Twenty-fourth Informational supplement. CLSI document M100- S24 Pennsylvania: CLSI.

Krumperman, P.H. (1983): Multiple Antibiotic Resistance Indexing of Escherichia coli to Identify High-risk Sources of Fecal Contamination of Foods, Appl Environ Microbiol 46 (1): 165-170. https://doi.org/10.1128/aem.46.1.165-170.1983.

Mc Hugh, M.L. (2012): Interrater reliability: the kappa statistic. Biochemia Medica 22 (3): 276-282.

Van Boeckel, T.P., Brower, C., Gilbert, M., Grenfell, B.T., Levin, S.A., Robinson, T.P., Teillant, A., Laxminarayan, R. (2015): Global trends in antimicrobial use in food animals. Proceedings of the National Academy of Sciences 112 (18): 5649-5654.

Parida, M., Sannarangaiah, S., Dash, P.K., Rao, P.V.L., Morita, K. (2008): Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol 18 (6): 407-421. https://doi.org/10.1002/rmv.593.

Saat, T. (2017): Amasya'da tüketime sunulan kıyma örneklerinden izole edilen Enterobacteriaceae üyelerinde B-laktamaz aktivitelerinin belirlenmesi (Master's thesis, Amasya Üniversitesi).

Öndeş, N. (2015): Kırmızı Et Örneklerinden Genişlemiş Spektrumlu Β Laktamaz (Gsbl) Üreten Enterobacteriaceae İzolatlarında Antibiyotik Dirençlilik Durumlarının İncelenmesi (Doctoral dissertation, İstanbul Aydın Üniversitesi Fen Bilimleri Enstitüsü).

Pehlivanlar Önen, S., Aslantaş, Ö., Yılmaz, E.Ş., Kürekci, C. (2015): Prevalence of β‐lactamase producing Escherichia coli from retail meat in Turkey. J Food Sci 80 (9): M2023-M2029. https://doi.org/10.1111/1750-3841.12984.

Abdallah, H.M., Al Naiemi, N., Elsohaby, I., Mahmoud, A.F., Salem, G.A., Vandenbroucke-Grauls, C.M.J.E. (2022): Prevalence of extended-spectrum β-lactamase-producing Enterobacterales in retail sheep meat from Zagazig city, Egypt. BMC Vet Res 18 (1): 1-7. https://doi.org/10.1186/s12917-022-03294-5.

Rajaei, M., Moosavy, M.H., Gharajalar, S.N., Khatibi, S.A. (2021): Antibiotic resistance in the pathogenic foodborne bacteria isolated from raw kebab and hamburger: phenotypic and genotypic study. BMC Microbiology 21 (1): 1-16. https://doi.org/10.1186/s12866-021-02326-8.

Dsani, E., Afari, E.A., Danso-Appiah, A., Kenu, E., Kaburi, B.B., Egyir, B. (2020): Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from raw meat in Greater Accra region, Ghana. BMC Microbiology 253. https://doi.org/10.1186/s12866-020-01935-z.

Trongjit, S., Angkittitrakul, S., Chuanchuen, R. (2016): Occurrence and molecular characteristics of antimicrobial resistance of Escherichia coli from broilers, pigs and meat products in Thailand and Cambodia provinces. Microbiol Immuno 60 (9):575–85.

Atlaw, N.A., Keelara, S., Correa, M., Foster, D., Gebreyes, W., Aidara-Kane, A. Harden, L., Thakur, S., Fedorka Cray, P.J. (2021): Identification of CTX-M Type ESBL E. coli from Sheep and Their Abattoir Environment Using Whole-Genome Sequencing. Pathogens 10 (11): 1480. https://doi.org/10.3390/pathogens10111480.

Solanki, R., Vanjari, L., Ede, N., Gungi, A., Soory, A., Vemu, L. (2013): Evaluation of LAMP assay using phenotypic tests and conventional PCR for detection of blaNDM-1 and blaKPC genes among carbapenem-resistant clinical Gram-negative isolates. Journal of Medical Microbiology 62 (10): 1540-1544.

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2024-02-06

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Halıcı Demir, C. ., & Kızıl, S. (2024). INVESTIGATION OF ESβL, AMP-C, CARBAPENEMASE PRODUCING ESCHERICHIA COLI STRAINS ANTIMICROBIAL RESISTANCE PROPERTIES ISOLATED FROM RED MEAT IN RETAIL BY MIC, PCR, AND LAMP PCR ANALYSIS. Journal of Applied Biological Sciences, 18(1), 64–77. Retrieved from https://jabsonline.org/index.php/jabs/article/view/1264

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