ANTIMICROBIAL EFFECT OF COMMERCIAL L. RHAMNOSUS STRAINS ON VANM-RESISTANT ENTEROCOCCI IN THE CHICKEN MEAT FILLET MODEL

Abstract views: 59 / PDF downloads: 33

Authors

  • Bahar Onaran Acar
  • Eray Burtaçgiray
  • İrem Turan

Keywords:

vanM-resistant E. faecium, LAB bacteria, Vancomycin-variable enterococci, Generally Recognized as Safe, Qualified Presumption of Safety

Abstract

Enterococci are ubiquitous bacteria and are of critical importance due to their ability to harbor antibiotic-resistance genes and their potential to transfer these genes to other bacteria. Vancomycin-resistant enterococci (VRE) are recognized as significant public health pathogens due to limited treatment options. The prevention of the transmission of vancomycin resistance by vanM-resistant enterococci to other pathogens, and the spread of this resistance gene to the environment are very significant for public health. Studies on the antimicrobial effect of Lactic acid bacteria (LAB) cultures on chicken meat are generally limited to Salmonella, Escherichia coli, and Listeria monocytogenes, and studies on inhibiting VRE are insufficient. In this context, the suppressive effect of commercially produced Lactobacillus rhamnosus cultures on vanM-resistant enterococci was investigated in this study. For this purpose, raw chicken fillet samples were contaminated with 4-6 log cfu/ml VRE, then dipped in a solution containing 9 log cfu/ml L. rhamnosus. On the application day (day 0), a decrease of 1.36 and 0.54 log cfu/ml was determined in the enterococcal counts in samples contaminated with 4-6 log cfu/ml VRE, respectively. Furthermore, on the 3rd day following the application, there was a decrease of 4 and 2.3 log cfu/ml in the VRE counts in the samples, respectively. After the application of L. rhamnosus solution, it was determined that there was more bacterial inhibition in samples with a bacterial density of 4 log cfu/ml than in samples with 6 log cfu/ml (p<0.05). Enterococci counts remained below the initial contamination at the end of the 3rd day following the application to the samples with both contaminations. When the results of the study were evaluated, it can be concluded that the commercially used L. rhamnosus cultures can be used to suppress VRE in poultry meat and products.

References

Zaheer, R., Cook, S. R., Barbieri, R., Goji, N., Cameron, A., Petkau, A., Polo, R. O., Tymensen, L., Stamm, C., Song, J., Hannon, S., Jones, T., Church, D., Booker, C. W., Amoako, K., Domselaar, G. V., Read, R. R., Mcallister, T.A. (2020): Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a One-Health continuum. Scientific Reports 10: 3937.

EU Commission Implementing Decision (2020): 2020/1729 of 17 November 2020 on the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria and repealing Implementing Decision 2013/652/EU. The Official Journal of the European Union L387/8.

Lee, T., Pang, S., Daley, D. A, Pearson, J. C, Abraham, S. Coombs, G. W. (2022): The changing molecular epidemiology of Enterococcus faecium harbouring the van operon at a teaching hospital in Western Australia: A fifteen-year retrospective study. Internation Journal of Medical Microbiology 312 (1): 151546.

Centers for Disease Control and Prevention (2019): Vancomycin-Resistant Enterococci (VRE), Antibiotic Resistance Threats in the United States, 2019. Atlanta, GA: U.S. Department of Health and Human Services, CDC [online]. Website http://dx.doi.org/10.15620/cdc:82532 [accessed 02.01.2022].

Ahmed, M. O., Baptiste, K. E. (2018): Vancomycin-resistant enterococci: a review of antimicrobial resistance mechanisms and perspectives of human and animal health. Microbial Drug Resistance 24 (5): 590-606.

Zhou, W., Zhou, H., Sun, Y., Gao, S., Zhang, Y., Cao, X., Zhang, Z., Shen, H., Zhang, C. (2020): Characterization of clinical enterococci isolates, focusing on the vancomycin-resistant enterococci in a tertiary hospital in China: based on the data from 2013 to 2018. BMC Infectious Diseases 20 (356): 1-9. https://doi.org/10.1186/s12879-020-05078-4

Chen, C., Sun, J., Guo, Y., Lin, D., Guo, Q., Hu, F., Zhu, D., Xu, X., Wang, M. (2015): High prevalence of vanM in vancomycin-resistant Enterococcus faecium isolates from Shanghai, China. Antimicrobial Agents and Chemotherapy 59: 7795-7798.

Hashimoto, Y., Taniguchi, M., Uesaka, K., Nomura, T., Hirakawa, H., Tanimoto, K., Tamai, K., Ruan, G., Zhenfg, B., Tomita, H. (2019): Novel multidrugresistant enterococcal mobile linear plasmid pELF1 encoding vanA and vanM gene clusters from a Japanese vancomycin-resistant enterococci isolate. Frontiers in Microbiology 10: 2568.

Sun, L., Qu, T., Wang, D., Chen, Y., Fu, Y., Yang, Q., Yu, Y. (2019): Characterization of vanM carrying clinical Enterococcus isolates and diversity of the suppressed vanM gene cluster. Infection, Genetics and Evolution 68: 145-152.

Onaran Acar, B., Cengız, G., Goncuoglu, M. (2023): Vancomycin-variable enterococci in sheep and cattle isolates and whole-genome sequencing analysis of isolates harboring vanM and vanB genes. Iranian Journal of Veterinary Research 24(3), 182-192.

Nalle, R. P. I., Nuraida, L., Mahakarnchanakul, W., Dewanti-Hariyadi R. (2021): Effect of sanitizers and Lactobacillus rhamnosus R23 on the growth of Salmonella spp. in raw chicken fillets during temperature abuse storage. Food Research 5: 250-258.

Azevedo, I., Barbosa, J., Albano, H., Nogueira, T., Teixeira, P. (2024): Lactic Acid Bacteria isolated from traditional and innovative alheiras as potential biocontrol agents. Food Microbiology 119, 104450.

Shi, C., Maktabdar, M. (2022): Lactic acid bacteria as biopreservation against spoilage molds in dairy products–A review. Frontiers in microbiology 12, 819684.

Samelis, J., Kakouri, A., Rogga, K. J., Savvaidis, I. N., Kontominas, M. G. (2003): Nisin treatments to control Listeria monocytogenes post-processing contamination on anthotyros, a traditional Greek whey cheese stored at 4 °C in vacuum packages. Food Microbiology 20 (6): 661-669.

Foulquie Moreno, M. R, Callewaert, R., Devreese, B., Van Beeumen, J., De Vuyst, L. (2003): Isolation and biochemical characterisation of enterocins produced by enterococci from different sources. Journal of Applied Microbiology 94 (2): 214-229.

Foulquie Moreno M. R., Rea M. C., Cogan, T. M., De Vuyst, L. (2003): Applicability of a bacteriocin-producing Enterococcus faecium as a co-culture in Cheddar cheese manufacture. International Journal of Food Microbiology 81 (1): 73-84.

Liu, L., O'Conner, P., Cotter, P. D., Hill, C., Ross, R. P. (2008): Controlling Listeria monocytogenes in Cottage cheese through heterologous production of enterocin A by Lactococcus lactis. Journal of Applied Microbiology 104 (4) 1059-1066.

Callewaert, R., Hugas, M., De Vuyst, L. (2000): Competitiveness and bacteriocin production of Enterococci in the production of Spanish-style dry fermented sausages. International Journal of Food Microbiology 57 (1-2): 33-42.

Ananou, S., Garriga, M., Hugas, M., Maqueda, M., Martinez-Bueno, M., Gálvez, A., Valdivia, E. (2005): Control of Listeria monocytogenes in model sausages by enterocin AS-48. International Journal of Food Microbiology 103 (2): 179-190.

Marcos, B., Aymerich, T., Monfort, J. M., Garriga, M. (2008): High-pressure processing and antimicrobial biodegradable packaging to control Listeria monocytogenes during storage of cooked ham. Food Microbiology 25 (1): 177-182.

Maragkoudakis, P. A, Mountzouris, K. C, Psyrras, D., Cremonese, S., Fischer, J. Cantor, M. D., Tsakalidou, E. (2009): Functional properties of novel protective lactic acid bacteria and application in raw chicken meat against Listeria monocytogenes and Salmonella enteritidis. International Journal of Food Microbiology 130 (3): 219-226.

Brashears, M. M, Reilly, S. S, Gilliland, S. E. (1998): Antagonistic action of cells of Lactobacillus lactis toward Escherichia coli O157: H7 on refrigerated raw chicken meat. Journal of Food Protection 61(2): 166-170.

Sakaridis, I., Soultos, N., Batzios, C., Ambrosiadis, I., Koidis, P. (2014): Lactic acid bacteria isolated from chicken carcasses with inhibitory activity against Salmonella spp. and Listeria monocytogenes. Czech Journal of Food Sciences 32 (1): 61-68.

Onaran Acar, B., Cengiz, G., Gülendağ, E., Göncüoğlu, M., Diker, K. (2022): Efficacy of peroxyacetic acid against Salmonella biofilms and as a decontamination agent in poultry meat. Journal of the Hellenic Veterinary Medical Society 73 (2): 4007-4014.

Wegener, H. C, Madsen, M., Nielsen, N., Aarestrup, F. M. (1997): Isolation of vancomycin resistant Enterococcus faecium from food. International Journal of Food Microbiology 35 (1): 57-66.

Sakaridis, I., Soultos, N., Dovas, C. I, Papavergou, E., Ambrosiadis, I., Koidis, P. (2012): Lactic acid bacteria from chicken carcasses with inhibitory activity against Salmonella spp. and Listeria monocytogenes. Anaerobe 2012; 18 (1): 62-66.

Matamoros, S., Pilet, M. F., Gigout, F., Prévost, H., Leroi, F. (2009): Selection and evaluation of seafood-borne psychrotrophic lactic acid bacteria as inhibitors of pathogenic and spoilage bacteria. Food Microbiology 26 (6): 638-644.

Downloads

Published

2024-06-21

How to Cite

Onaran Acar, B., Burtaçgiray, E., & Turan, İrem. (2024). ANTIMICROBIAL EFFECT OF COMMERCIAL L. RHAMNOSUS STRAINS ON VANM-RESISTANT ENTEROCOCCI IN THE CHICKEN MEAT FILLET MODEL. Journal of Applied Biological Sciences, 18(2), 193–200. Retrieved from https://jabsonline.org/index.php/jabs/article/view/1302

Issue

Section

Articles