INVESTIGATION OF TOXOPLASMA GONDII AND SARCOCYSTIS SPP. TISSUE CYSTS IN MEAT PRODUCTS AVAILABLE TO THE MARKET BY PERCOLL GRADIENT METHOD
DOI:
https://doi.org/10.71336/jabs.1330Keywords:
Meat, percoll gradient, sarcocystis, tissue cyst, Toxoplasma gondiiAbstract
Purpose of this study is microscopic examination of Toxoplasma gondii and Sarcocystis spp. in lamb meat, beef, meatball and sausages launched for retail sale in Ankara and Kırıkkale using Percoll Gradient method. By visiting butchers in Ankara and Kırıkkale 50 pieces of each meat products (200 total) were purchased. After 5 grams of the samples were homogenized, they were subjected to the Percoll gradient method. Ten coverslip areas from each sample were examined under the light microscope for the presence of T. gondii and Sarcocystis spp. tissue cysts. In 78.5% of the 200 samples that examined there was at least one parasite species tissue cyst. In 2.5 % of total samples had T. gondii and 56.5% had Sarcocystis spp. and 19.5% had both T. gondii+Sarcocystis spp. tissue cysts. 95% of lamb meats, 62% of beef, 90% of meatballs and 68% of sausages were found to be infected with at least one species. In conclusion, T. gondii and Sarcocystis spp. tissue cysts were found on meat products that launched in Kırıkkale and Ankara. Because of the zoonotic feature of T.gondii it is highly important to consume meat products after cooked well for public health. And for prevent meat products from getting Sarcocystis spp. tissue cysts, it is important to take cautions for our animals to not get infected.
References
Dubey, J.P. (2010): Toxoplasmosis of Animals and Humans. 2nd ed. CRC Press, Boca Raton, FL, USA. pp. 1-71.
Buxton, D. (1998): Protozoan infections (Toxoplasma gondii, Neospora caninum and sarcocystis spp.) in sheep and goats: Recent advances. Veterinary Research 29(3–4): 289–310.
Robert-Gangneux, F., Dard´e, M.L. (2012): Epidemiology of and diagnostic strategies for toxoplasmosis. Clinical Microbiology Reviews, 25(2): 264–296. DOI: https://doi.org/10.1128/CMR.05013-11
Ahmadiara, E., Rahimzadeh, A., Samakkhah, S.A. (2023): Identification of Sarcocystis spp. in hamburgers distributed in Fouman City, North of Iran using dab smear and digestion methods. Journal of Environmental Health and Sustainable Development 8(4): 2134-2138. DOI: https://doi.org/10.18502/jehsd.v8i4.14439
Dubey, J.P., Lindsay, D.S. (2006): Neosporosis, toxoplasmosis, and sarcocystosis in ruminants. Veterinary Clinics of North America: Food Animal Practice 22(3):645-671. DOI: https://doi.org/10.1016/j.cvfa.2006.08.001
Aydenizöz Özkayhan, M., Karaer, Z., İlkme, A.N., Atmaca, H.T. (2007): Kırıkkale Belediye mezbahasında kesilen koyunlarda Sarcocystis türlerinin yaygınlığı. Türkiye Parazitoloji Dergisi 31 (4): 272-276.
Singla, L.D, Juyal, P.D. (2014): Sarcocystosis. In: Garg SR (ed) Zoonosis: parasitic and mycotic diseases. Daya Publishing House, New Delhi. pp. 235–250.
Fayer, R. (2004). Sarcocystis spp. in human infections. Clinical Microbiology Review 17(4):894–902. DOI: https://doi.org/10.1128/CMR.17.4.894-902.2004
Hill, D.E., Dubey, J.P. (2016): Toxoplasma gondii as a parasite in food: Analysis and control. Microbiology Spectrum 4(4). DOI: https://doi.org/10.1128/microbiolspec.PFS-0011-2015
Fayer, R., Esposito, D.H., & Dubey, J.P. (2015): Human infections with Sarcocystis species. Clinical Microbiology Reviews 28(2): 295–311 DOI: https://doi.org/10.1128/CMR.00113-14
Yildiz, K., Piskin, F.C., Utuk, A.E., Gokpinar, S. (2015): Prevalence of Toxoplasma gondii in sheep meats purchased from retail stores in Central Anatolia, Turkey. Turkish Journal of Veterinary and Animal Sciences 39:328-332. DOI: https://doi.org/10.3906/vet-1412-63
Muz, M.N., Kurultay, Ş., Gümüş, T., Vural, G. (2006): Kırmızı et ve ürünlerinde Toxoplasma gondii’nin Nested PCR metodu ile tespit edilmesi ve genotiplendirilmesi. Proje Sonuç Raporu. Namık Kemal Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi. Tekirdağ, Türkiye.
Ergin, S., Ciftcioglu, G., Midilli, K., Issa, G., Gargili, A. (2009): Detection of Toxoplasma gondii from meat and meat products by the Nested-PCR method and its relationship with seroprevalence in slaughtered animals. Bulletin of the Veterinary Institute in Pulawy 53: 657-661.
Plaza, J., Damek, F., Villena, I., Innes, E.A., Katzer, F., Hamilton, C.M. (2020): Detection of Toxoplasma gondii in retail meat samples in Scotland. Food and Waterborne Parasitology 20: e00086. DOI: https://doi.org/10.1016/j.fawpar.2020.e00086
Rahdar, M., Samarbaf-Zadeh, A.R., Arab, L. (2012): Evaluating the prevalence of Toxoplasma gondii in meat and meat products in Ahvaz by PCR method. Jundishapur Journal of Microbiology 5(4): 570-573. DOI: https://doi.org/10.5812/jjm.4280
Amairia, S., Rouatbi, M., Rjeibi, M.R., Gomes, J., Rekik, M., Darghouth, M.A., Gharbi, M. (2021): Molecular detection of Toxoplasma gondii and Sarcocystis spp. co-infection in Tunisian Merguez, a traditional processed sausage beef meat. Food Control 121:107618. DOI: https://doi.org/10.1016/j.foodcont.2020.107618
Dehkordi, Z.S., Yalameha, B., Sari, A.A. (2017): Prevalence of Sarcocystis infection in processed meat products by using digestion and impression smear methods in Hamedan, Iran. Comparative Clinical Pathology 26:1023-1026. DOI: https://doi.org/10.1007/s00580-017-2478-3
Mavi, S.A., Teimouri, A., Mohebali, M., Yazdi, M.K.S., Shojaee, S., Rezaian, M., Salimi, M., Keshavarz, H. (2020): Sarcocystis infection in beef and industrial raw beef burgers from butcheries and retail stores: A molecular microscopic study. Heliyon 6: e04171. DOI: https://doi.org/10.1016/j.heliyon.2020.e04171
Maky, M.A., Mohammed, E.S. (2021): Molecular identification of Sarcocystis spp. in meat and meat products in Qena, Upper Egypt. Journal of Animal Health and Production 9(1): 88-93. DOI: https://doi.org/10.17582/journal.jahp/2021/9.1.88.93
Rahdar, M., Salehi, M. (2011): The prevalence of Sarcocystis infection in meat-production by using digestion method in Ahvaz, Iran. Jundishapur Journal of Microbiology 4(4): 295-299.
Kamber, U., Arslan, M.Ö., Gülbaz, G., Taşçı, G.T., Akça, A. (2018): Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage). Turkish Journal of Veterinary & Animal Sciences 42 (1): 1-6. DOI: https://doi.org/10.3906/vet-1705-84
Sancak, Y.C., Akdemir, C., Berktaş, M. (1995): Van piyasasında tüketime sunulan et ve et ürünlerinde Sarcosporidiosis’in yayılışı. Yüzüncü Yıl Üniversitesi Sağlık Bilimleri Dergisi 2: 114-116.
Hussein, D.E., Abu-Akkada, S.S., Bessat, M.S., Aggour, M.G., Otify, Y.Z. (2017): Molecular identification of sarcocystis species in imported frozen beef in Egypt. Alexandria Journal of Veterinary Sciences 53 (2):72-82. DOI: https://doi.org/10.5455/ajvs.244449
Xue, R., Yan, W., Qian, W., Wang, T., Zhang, M., Wei, Z., Han, L., He, B., Dou, J. (2019): Prevalence and molecular characterization of Sarcocystis infections of retail beef products from central China. Acta Tropica 190:339-343. DOI: https://doi.org/10.1016/j.actatropica.2018.12.015
Jafari, F., Motavallihaghi, S.Y., Bakhtiari, M., Maghsood, A.H., Foroughi-Parvar, F. (2022): Sarcocystis bovifelis in raw hamburgers marketed in Hamadan City, Western Iran. Iranian Journal of Parasitology 17 (1):36-42. DOI: https://doi.org/10.18502/ijpa.v17i1.9014
Oğuz, B., Değer, M.S., Koşal, S. (2021): Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey. Ankara Universitesi Veteriner Fakültesi Dergisi 68 (2):97-105. DOI: https://doi.org/10.33988/auvfd.671606
Gokpinar, S., Yildiz, K., Gurcan, I.S. (2014): Prevalence and concentration of Sarcocystis spp. microscopic cysts in sheep muscles using percoll gradient centrifugation. Israel Journal of Veterinary Medicine 69 (1):16-19.
Gencay, Y.E., Yildiz, K., Gokpinar, S., Leblebicier, A. (2013): A potential infection source for humans: Frozen buffalo meat can harbour tissue cysts of Toxoplasma gondii. Food Control 30: 86-89. DOI: https://doi.org/10.1016/j.foodcont.2012.07.021
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