THE EFFECT OF DIFFERENT CONCENTRATIONS OF ACETOSYRINGONE ON TRANSFERRING rol GENES TO SOME CITRUS ROOTSTOCKS VIA Agrobacterium rhizogenes

Abstract

Genetic engineering technologies have been taken part in the breeding strategies of fruit crops. Several biotechnological methods can be applied to plant to have better ones in the process of fruit breeding. Among these methods, Agrobacterium-mediated gene transfer is frequently used. Citrus species are the most widely grown fruit crops. Despite substantial genetic diversity and interspecific fertility, the genus Citrus includes some of the most difficult species to breed. This is due to several obstacles for conventional breeding. For this reason, biotechnological breeding methods are used in citrus breeding. Acetosyringone is one of the phenolic compounds secreted by injured plant cells and affects the transformation efficiency. This study aims to determine the effect of different acetosyringone concentrations (0, 25, 50, 100 and 200 µM) on the transformation of rol genes in citrus genotypes and check whether the plantlets obtained after transformation carry the rol genes by molecular methods. Tuzcu 3131 sour orange, Gou Tou sour orange, Carrizo citrange, Rough lemon, Volkameriana, Trifoliate orange were used in this study. As a result, the highest shoot formation was obtained at 200 µM acetosyringone concentration and the highest root formation was obtained at 25 µM acetosyringone concentration