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  • Chaitra R. Sharma
  • Praveen Kumar S. Kondaguli
  • Laxmi S. Inamdar Karnatak University


Prolactin receptor, Mouse, Oviduct, Ampulla, Isthmus


In mice, the oviduct is required for the timely accomplishment of gamete transport, fertilization, preimplantation embryo development, and to deliver a competent and healthy conceptus to the endometrium. Apart from the ovarian steroids, prolactin (PRL)from hypophysis acting through prolactin receptor (PRLR) plays a major role during early pregnancy. The present study is aimed to know the spatiotemporal localization of PRLR in the mouse oviduct during the pre- and peri-implantation phases of early pregnancy. Oviducts were collected from mice during different phases of early pregnancy, fixed in 4% paraformaldehyde, subjected to immunohistochemistry and western blotting. The intensities of immunohistochemical staining were measured in the oviductal subregions using ImageJ (Fiji) software. The expression of PRLR was principally found in the apical membrane and cytoplasm of oviductal cells, irrespective of its subregions (infundibulum, ampulla, and isthmus). Nevertheless, the intensity of PRLR varies throughout early pregnancy. The highest expression of PRLR was observed in the ciliated epithelial cells of the infundibulum and ampullary region of the oviduct on gestation day (GD) 1.5 and 2.5. However, downregulation of PRLR in the oviductal epithelial cells was noticed during the peri- and post-implantation periods. The diminished PRLR expression in the epithelial cells was maintained thereafter. Western blot analysis revealed a single protein band with an apparent molecular weight of 90 kDa. It is inferred that time specific expression pattern of PRLR along the oviductal subregions indicates the requirement of prolactin hormone and its receptors during the preimplantation period.


Maillo, V., Lopera-Vasquez, R., Hamdi, M., Gutierrez-Adan, A., Lonergan, P., Rizos, D. (2016a): Maternal-embryo interaction in the bovine oviduct: Evidence from in vivo and in vitro studies. Theriogenology 86: 443-450.

Maillo, V., Sanchez-Calabuig, M. J., Lopera-Vasquez, R., Hamdi, M., Gutierrez-Adan, A., Lonergan, P., Rizos, D. (2016b): Oviductal response to gametes and early embryos in mammals. Reproduction 152: R127-141.

Alminana, C., Bauersachs, S. (2019): Extracellular vesicles in the oviduct: progress, challenges and implications for the reproductive success. Bioengineering 6: 32.

Li, S., Winuthayanon, W. (2017): Oviduct: roles in fertilization and early embryo development. Journal of Endocrinology 232: 1-26.

Edwards, R. G., Fishel, S. B., Cohen,J., Fehilly,C. B., Purdy, J. M., Slater,J. M., Steptoe, P. C., Webster J. M.(1984): Factors influencing the success of in vitro fertilization for alleviating human infertility Journal of In Vitro Fertilization and Embryo Transfer 1: 3-23.

Arslan, H. O., Herrera, C., Malama, E., Siuda, M., Leiding, C., & Bollwein, H. (2019): Effect of the addition of different catalase concentrations to a TRIS-egg yolk extender on quality and in vitro fertilization rate of frozen-thawed bull sperm. Cryobiology.

Freeman, M. E., Kanyicska, B., Lerant, A., Nagy, G. (2000): Prolactin: structure, function, and regulation of secretion. Physiological Reviews. 80: 1523-631.

Cunningham, B. C., Henner, D. J., Wells, J. A. (1990): Engineering human prolactin to bind to the human growth hormone receptor. Science 247: 1461-1465.

Bachelot, A., Binart, N. (2007): Reproductive role of prolactin. Reproduction 133: 361-369.

Ben-Jonathan, N., Lapensee, C.R., Lapensee, E. W. (2008): What can we learn from rodents about prolactin in humans? Endocrine Reviews 29: 1-41.

Forsyth, I. A. (1986): Variation Among Species in the Endocrine Control of Mammary Growth and Function: The Roles of Prolactin, Growth Hormone, and Placental Lactogen.Journal of Dairy Science 69:886-903.

Grattan, D. R. (2001): The actions of prolactin in the brain during pregnancy and lactation. Progress in Brain Research 133: 153-171.

Woodside, B., Augustine, R. A., Ladyman, S. R., Naef, L., Grattan, D. R. (2008): Role of prolactin in the metabolic adaptations to pregnancy and lactation. Bridges RS. (ed.), Neurobiology of the Parental Brain San Diego: Academic Press 249-268.

Bazan, J.F. (1989): A novel family of growth factor receptors: a common binding domain in the growth hormone, prolactin, erythropoietin and IL-6 receptors, and the p75 IL-2 receptor beta-chain. Biochemical and Biophysical Research Communications 164: 788-795.

Bole-Feysot, C., Goffin, V., Edery, M., Binart, N., Kelly, P. A. (1998): Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocrine Reviews 19: 225-268.

Bachelot, A.,Binart N. (2007): Reproductive role of prolactin. Reproduction 133:361–369

Shao, R., Nutu, M., Weijdegard, B., Egecioglu, E., Fernandez-Rdriguez, J., Tallet, E., Goffin, V., Ling, C., Billig, H. (2008): Differences in prolactin receptor (PRLR) in mouse and human fallopian tubes: Evidence for multiple regulatory mechanisms controlling PRLR isoform expression in mice. Biology of Reproduction 79: 748-757.

Ormandy, C. J., Camus, A., Barra, J. Damotte, D., Lucas, B., Buteau, H., Edery, M., Brousse, N., Babinet, C., Binart, N., Kelly,P. A. (1997): Null mutation of the prolactin receptor gene produces multiple reproductive defects in the mouse. Genes and Development 11: 167-178.

Clarke, D. L. Linzer, D. I. (1993): Changes in prolactin receptor expression during pregnancy in the mouse ovary. Endocrinology 133: 224-232.

Clarke, D. L., Arey, B. J., Linzer, D. I. (1993): Prolactin receptor messenger ribonucleic acid expression in the ovary during the rat estrous cycle. Endocrinology 133: 2594-2603.

Tzeng, S. J., Linzer, D. I. (1997): Prolactin receptor expression in the developing mouse embryo. Molecular Reproduction Development 48: 45-52.

Kiapekou, E., Loutradis, D., Patsoula, E., Koussidis, G. A., Minas, V., Bletsa, R., Antsaklis, A., Michalas, S., Makrigiannakis, A. (2005): Prolactin receptor mRNA expression in oocytes and preimplantation mouse embryos. Reproductive Biomedicine Online 10: 339-346.

Reese, J., Binart, N., Brown, N., Ma, W. G., Paria, B. C., Das, S. K., Kelly, P. A. Dey, S. K. (2000): Implantation and decidualization defects in prolactin receptor (PRLR)-deficient mice are mediated by ovarian but not uterine PRLR. Endocrinology 141:1872-1881.

Lebrun, J. J., Ali, S., Sofer, L., Ullrich, A., Kelly, P. A. (1994): Prolactin-induced proliferation of Nb2 cells involves tyrosine phosphorylation of the prolactin receptor and its associated tyrosine kinase JAK2. Journal of Biological Chemistry 269:14021–14026 12.

Rui, H., Kirken, R. A., Farrar, W. L. (1994): Activation of receptor-associated tyrosine kinase JAK2 by prolactin. Journal of Biological Chemistry269:5364–5368.

Barkley, M. S., Bradford, G. E., Geschwind, I. I. (1978): Pattern of plasma prolactin concentration during the first half of mouse gestation. Biology of Reproduction 19: 291-296.

Yohkaichiya, T., Fukaya, T., Hoshiai, H., Yajima, A. (1988): Improvement of Mouse Embryo Development In Vitro by Prolactin. Journal of Experimental Medicine 155: 241-246

Charoenphandhu, K., Krishnamra, N. (2007): Prolactin is an important regulator of intestinal calcium transport. Canadian Journal of Physiology and Pharmacology 85: 569-581.

Barton, B. E., Herrera, G. G., Anamthathmakula, P., Rock, J. K., Willie, A. M., Harris, E. A., Takemaru, K. I., Winuthayanon, W. (2020): Roles of steroid hormones in oviductal function. Reproduction159:125–137.

Hewitt, S. C., Winuthayanon, W., Korach, K. S. (2016): What’s new in estrogen receptor action in the female reproductive tract. Journal of Molecular Endocrinology 56:55–71.

Conneely, O. M., Mulac-Jericevic, B., Lydon, J. P. (2003): Progesterone-dependent regulation of female reproductive activity by two distinct progesterone receptor isoforms. Steroids 68:771–778.

Mulac-Jericevic, B., Lydon, J. P., Demayo, F. J., Conneely, O. M. (2003): Defective mammary gland morphogenesis in mice lacking the progesterone receptor b isoform. PNAS 100: 9744–9749.

Gidley-Baird, A.A. (1977): Plasma Progesterone, FSH and LH Levels Associated with Implantation in the Mouse. Australian Journal of Biological Sciences.30: 289-96.

Al-Chalabi,M., Bass, A. N.,Alsalman,I.(2021): Physiology, Prolactin In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing.

Buser, A. C.,Gass-Handel, E. K.,Wyszomierski, S. L., Doppler,W., Leonhardt,S. A., Schaack,J., Rosen,J. M., Watkin, H., Anderson, S. M., Edwards, D. P. (2007): Progesterone Receptor Repression of Prolactin/Signal Transducer and Activator of Transcription 5-Mediated Transcription of the β-Casein Gene in Mammary Epithelial Cells. Molecular Endocrinology 21: 106–125.




How to Cite

Sharma, C. R., Kondaguli, P. K. S., & Inamdar, L. S. (2022). SPATIOTEMPORAL EXPRESSION OF PRLR IN MURINE OVIDUCT DURING PREIMPLANTATION PERIOD. Journal of Applied Biological Sciences, 16(1), 35–45. Retrieved from