INVESTIGATION OF SYNERGISTIC EFFECT OF GELDANAMYCIN AND CUCURBITACIN ON HUMAN COLON CANCER

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Authors

  • Mustafa Cellat Department of Physiology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey
  • Ares Alizade Department of Pharmacology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, Turkey
  • Halil Mahir Kaplan Department of Pharmacology, Faculty of Medicine, Çukurova University, Adana, Turkey
  • Gülşah Evyapan Department of Medical Biology and Genetics, Faculty of Medicine, Cukurova University, Adana, Turkey
  • Memet Güvenç Department of Physiology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey

Keywords:

Cucurbitacin, Geldanamycin, colon cancer, apoptosis, synergy

Abstract

Colon cancer is one of the most common cancers observed in men and women in the world, today in many countries after cardiovascular diseases; it is second leading cause of death. In this the study we aimed to investigate the effects of use of Cucurbitacin I and together with Geldanamycin (GA) which have apoptotic and anti-proliferativeeffects on HT29. Cells were treated with GA, CU and GA+CU. Effect of GA, CU on cell viabilitywere demonstrated by MTT assay and activities of bax, caspase-3, bcl-2, GRP78, wee1, AIF and GAAD153 were performedwith ELISA method after 48 hour treatment. Our study showed that the combined use of GA and CU significantly reduced cell viability of colon cells. Also, the same dose of GA and CU used together increased the expression of proapoptotic proteins and decreased anti-apoptotic proteins more than their effects alone. As aconclusion, our study showed that GA and CU synergistically induce apoptosis on humancolon cancer.

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Published

2022-05-29

How to Cite

Cellat, M., Alizade, A., Kaplan, H. M., Evyapan, G., & Güvenç, M. (2022). INVESTIGATION OF SYNERGISTIC EFFECT OF GELDANAMYCIN AND CUCURBITACIN ON HUMAN COLON CANCER. Journal of Applied Biological Sciences, 16(2), 248–256. Retrieved from https://jabsonline.org/index.php/jabs/article/view/997

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