EVALUATION OF CHEMICAL COMPOSITION, ANTI-INFLAMMATORY, ANTIBACTERIAL ACTIVITY AND SYNERGISTIC EFFECT BETWEEN ANTIBIOTICS AND THE ESSENTIAL OIL OF ARTEMISIA CAMPESTRIS L.
DOI:
https://doi.org/10.71336/jabs.1002Keywords:
Artemisia campestris, essential oil, hydro distillation, chemical composition, synergies, antibacterial activity, acute toxicity, anti-inflammatory activityAbstract
This present study aimed to elucidate the chemical composition of essential oil (EO) which was obtained from the leaves of Artemisia campestris (Asteraceae) collected in Djelfa region (Algeria). The in-vitro antibacterial activity against six (06) bacterial strains were evaluated, the EO was used alone and associated with antibiotics to assess their synergistic effect. The in-vivo acute toxicity and anti-inflammatory activity were evaluated. In this work thirty-three (33) compounds accounting for 100% of total oil were identified by GC and GC/MS analysis of the essential of A. campestris. Camphor (41.95%), Chrysanthenone (13.95%), and 1,8-Cineole (13.31%) are found to be the major compounds. From the results of the antibacterial activity using disc diffusion method revealed inhibition zones ranging from 18.33 ±0.57 to 24.33± 0.57mm. Gram-negative was more sensitive to EO compared to Gram-positive bacteria. The combined application of EO of the studied specie with standard antibiotics led to a synergistic effect in some bacteria. At the highest tested dose (5000 mg/kg p.o.) the EO of A. campestris did not show signs of acute toxicity. EO of A. campestris reduced significantly the paw edema induced by carrageenan in mice at 27.36%, 39.62%, and 56.60% (after 6 hours) at the doses of 100, 200, and 400 (mg/kg) respectively.
References
Grimsrud, P.A., Xie, H., Griffin, T.J., Bernlohr, D.A. (2008): Oxidative stress and covalent modification of protein with bioactive aldehydes. J Biol Chem 283: 21837–21841. DOI: https://doi.org/10.1074/jbc.R700019200
Lozniewski, A., Rabaud, C., Nancy. Bacterial antibiotic resistance. (2010): Lyon: CCLIN Sud-Est. French.
Cushnie, T.P.T., Lamb, A.J. (2011): Recent advances in understanding the antibacterial properties of flavonoids. International journal of antimicrobial agents 38(2): 99–107. DOI: https://doi.org/10.1016/j.ijantimicag.2011.02.014
Sudano, R.A., Rita Blanco, A., Giuliano, F., Rusciano, D., Enea V. (2004): Epigallocatechin-Gallate Enhances the Activity of Tetracycline in Staphylococci by Inhibiting Its Efflux from Bacterial Cells. Antimicrobial Agents and Chemotherapy 48(6): 1968–1973. DOI: https://doi.org/10.1128/AAC.48.6.1968-1973.2004
Fellah, S., Romdhane & Abderraba, M. (2006): Extraction and study of essential oils from Salvia officinalis L. collected in two different regions of Tunisia. J. Soc. Alger. Chim 16 (2): 193−202.
Zeggwagh, N.A., Michel, J.B & Eddouks, M. (2014): Acute hypotensive and diuretic activities of Artemisia herba-alba aqueous extract in normal rats. Asian Pacific Journal of Tropical Biomedicine. DOI: https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0136
Jakovljević, M.R., Grujičić, D., Vukajlović, J.T., Marković A., Milutinović M., Stanković, M., Vuković, N., Vukić, M., & Milošević-Djordjević, O. (2020): In-vitro study of genotoxic and cytotoxic activities of methanol extracts of Artemisia vulgaris L. and Artemisia alba Turra. South African Journal of Botany 132: 117–126. DOI: https://doi.org/10.1016/j.sajb.2020.04.016
Mansouri, A., Embarek, G., Kokkalou, E & Kefalas, P. (2005): Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chemistry 89(3): 411–420. DOI: https://doi.org/10.1016/j.foodchem.2004.02.051
Shahidi, F. & Ambigaipalan P. (2015): Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects. A review. Journal of Functional Foods 1–78. DOI: https://doi.org/10.1016/j.jff.2015.06.018
Ozcan, T., Akpinar-Bayizit, A., Yilmaz-Ersan, L. & Delikanli, B. (2014): Phenolics in human health. International Journal of Chemical Engineering and Applications 5(5): 393–396. DOI: https://doi.org/10.7763/IJCEA.2014.V5.416
Ziani, B.E.C., Barros, L., Boumehira, A.Z., Bachari, K., Heleno, S.A., Alves, M.J. & Ferreira, I.C.F.R. (2018): Profiling polyphenol composition by HPLC-DAD-ESI/MSn and the antibacterial activity of infusion preparations obtained from four medicinal plants. Food & Function 9: 149–159. DOI: https://doi.org/10.1039/C7FO01315A
Mabberley, D.J. (1998): The Plant-Book. UK, Cambridge University press Cambridge,
Quezel, P., Santa, S. (1963): New flora from Algeria and southern desert regions. CNRS, tome 2, Paris, 1170p.
Baba Aissa, F. (1991): Medicinal plants in Algeria. Co´dition Bouche´ne´ and Ad. Diwan, Algiers, Alge´rie.
Wu, H., Guo, J., Chen, S., Liu, X., Zhou, Y., Zhang, X & Xu, X. (2013): Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–Mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 72(151): 267–291. DOI: https://doi.org/10.1016/j.jpba.2012.09.004
Akrout, A., El Jani, H., Amouri, S., Neffati, M. (2010): Screening of antiradical and antibacterial activities of essential oils of Artemisia campestris l., Artemisia herba alba asso, & Thymus capitatus hoff. Et link. Growing wild in the southern of Tunisia. Recent Research in Science and Technology 2(1): 29–39.
Neffati, A., Skandrani, I., Sghaier, M.B., Bouhlel, I., Kilani, S., Ghedira, K., Neffati, M., Cherif, I., Hammami, M., Chekir-Ghedira, L. (2008): Chemical composition, mutagenic and antimutagenic activities of essential oils from (Tunisian) Artemisia campestris and Artemisia herba-alba. J. Essent. Oil. Res 20(5): 471-477.
Mucciarelli, M., Caramiello, R., Maffei, M., Chialva, F. (1995): Essential oils from some Artemisia species growing spontaneousl in North-West Italy. Flav Fragr J 10: 25–32. DOI: https://doi.org/10.1002/ffj.2730100105
Bellomaria, B., Valentini, G., Biondi, E. (2001): Chemotaxonomy of Artemisia variabilis Ten. and A. campestris. ssp. glutinosa (Ten.) Briq. et Cavill. (Asteraceae) from Italy. J Essent Oil Res 13: 90–94. DOI: https://doi.org/10.1080/10412905.2001.9699623
Akrout, A., Chemli, R., Cherı¨f, I., Hammami, M. (2001): Analysis of the essential oil of Artemisia campestris. Flav Frag J 16: 337–339. DOI: https://doi.org/10.1002/ffj.1006
Dob, T., Dahmane, D., Berramdane, T., and Chelghoum, C. (2005): Chemical Composition of the Essential Oil of Artemisia campestris L. from Algeria. Pharmaceutical Biology Vol. 43, No. 6, pp: 512–514. DOI: https://doi.org/10.1080/13880200500220664
Hatimi, S., Boudouma, M., Bichichi, M., Chaib, N., Idrissi, N.G. (2000): Evaluation in-vitro of anti-leishmanien activity of Artemisia herba-alba Asso (in French). Presented at Franco-African meeting of pediatrics N°14, Paris, France. Exotic pathology Society: Paris, France, pp. 57-70.
Djeridane, A., Yousfi, M., Nadjemi, B., Boutasouma, D & Vidal, N. (2006): Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chimistry 97(4): 654–660. DOI: https://doi.org/10.1016/j.foodchem.2005.04.028
Aniya, Y., Shimabukuro, M., Shimoji, M., Kohatsu, M., Gyamfi, M.A., Miyagi, C.D, Kunii Takayama, F., Egashira, T. (2000): Antioxidant and Hepatoprotective Actions of the Medicinal Herb Artemisia campestris from the Okinawa Islands. Biological and Pharmaceutical Bulletin 23(3): 309-312. DOI: https://doi.org/10.1248/bpb.23.309
Tharib, S.M., Gnan, S.O., Veitch, G.B.A. (1983): Antimicrobial activity of compounds from Artemisia campestris. J. Food. Prot 46: 681-685. DOI: https://doi.org/10.4315/0362-028X-46.3.185
Ghaleb, A., Mhanna, M. (2008): Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Middle East J Sci Res 3(3): 134-9.
Olajuyigbe, OO., Afolayan, A.J. (2012): Synergistic interactions of methanolic extract of Acacia mearnsii De Wild. with antibiotics against bacteria of clinical relevance. Int J Mol Sci 13: 8915-32. DOI: https://doi.org/10.3390/ijms13078915
Batlouni, M. (2010): Nonsteroidal anti-inflammatory drugs: cardiovascular, cerebrovascular and renal effects. Arquivos Brasileiros de Cardiologia 94(4): 522–563. DOI: https://doi.org/10.1590/S0066-782X2010000400019
Bengmark, S. (2004): Acute and "chronic" phase reaction-a mother of disease. Clinical nutrition 23(6):1256–1266. DOI: https://doi.org/10.1016/j.clnu.2004.07.016
Széles, L., Töröcsik, D., and Nagy, L. (2007): PPAR γ in immunity and inflammation: cell types and diseases. Biochimica et Biophysica Acta (BBA)-Mol. Cell Biol. Lipids 1771(8): 1014-1030. DOI: https://doi.org/10.1016/j.bbalip.2007.02.005
Kang, S.M. Kim, K.N., Lee, S.H., Ahn, G., Cha, S.H., Kim, A.D. and Jeon, Y.J. (2011): Anti-inflammatory activity of polysaccharide purified from AMG-assistant extract of Ecklonia cava in LPS-stimulated RAW 264.7 macrophages. Carbohydrate Polymers 85(1): 80-85. DOI: https://doi.org/10.1016/j.carbpol.2011.01.052
Iwalewa, E.O., McGaw, L.J., Naidoo, V., and Eloff, J.N. (2007): Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. African Journal of Biotechnology 6(25). DOI: https://doi.org/10.5897/AJB2007.000-2457
Abdelli, W., Bahri, F., Romane, A., Höferl, M., Wannerd, J., Schmidtc, E., and Jirovetzc, L. (2017): Chemical Composition and Anti-inflammatory Activity of Algerian Thymus vulgaris Essential Oil. Natural Product Communications, pp 611 – 614. DOI: https://doi.org/10.1177/1934578X1701200435
Akrout, A., Gonzalez, L.A., El Jani, H & Madrid, P.C. (2011): Antioxidant and antitumor activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia. Food. Chem. Toxicol 49: 342-347. DOI: https://doi.org/10.1016/j.fct.2010.11.003
Adams, R.P. (2007): Identification of essential oil components by gas chromatography/mass spectorscopy (4th ed). Allured Pub. Corp.
König, W.A., Joulain, D. & Hochmuth, D.H. (2001): GC/MS Library: Terpenoids and Related Constituents of Essential Oils.
Babushok, VI., Linstrom, P.J., Zenkevich, I.G. (2011): Retention Indices for Frequently Reported Compounds of Plant Essential Oils. J. Phys. Chem. Ref. Data Vol. 40, No. 4. DOI: https://doi.org/10.1063/1.3653552
Van Den Dool, H., Dec Kratz, P. (1963): A generalization of the retention index system including linear temperature programmed gas—liquid partition chromatography. Journal of Chromatography A. 11: 463–471. DOI: https://doi.org/10.1016/S0021-9673(01)80947-X
Perveen, K., Bokhari, N.A. & Dina Soliman, A.W. (2012): Antibacterial activity of Phoenix dactylifera L. leaf and pit extracts against selected Gram-negative and Gram-positive pathogenic bacteria. Journal of Medicinal Plants Research 6(2): 296–300. DOI: https://doi.org/10.5897/JMPR11.1380
Lesueur, D., de Rocca Serra, D., Bighelli, A., Hoi, T.M., Ban, N.K., Thai, T.H. (2007): Chemical composition and antibacterial activity of essential oil of Michelia faveolata Meryll ex Dandy from Vietnam. Flavour Fragr J 22: 317-21. DOI: https://doi.org/10.1002/ffj.1799
Toroglu, S. (2007): In-vitro antimicrobial activity and antagonistic effect of essential oils from plant species. J Environ Biol 28: 551-9.
Faria, L.R.D., Lima, C.S., Perazzo, F.F., Carvalho, J.C.T. (2011): Anti-inflammatory and antinociceptive activities of the essential oil from Rosmarinus officinalis L. (Lamiaceae). International Journal of Pharmaceutical Sciences Review and Research 7(2): 1-8p.
OECD. (2002): Test No. 423: Acute Oral Toxicity-Acute Toxic Class Method. Organization for Economic Co-operation and Development, Paris.
Winter, C.A., Risley, E.A., Nuss, G.W. (1962): Carrageenin-induced edema in hind paw of the ratas an assay for anti-inflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y) 111: 544-547p. DOI: https://doi.org/10.3181/00379727-111-27849
Vasudevan, M., Gunnam, K.K. Parle, M. (2006): Antinociceptive and anti-inflammatory properties of Daucus carota seeds extract. Journal of Health Science 52(5): 598-606p. DOI: https://doi.org/10.1248/jhs.52.598
Touil, S., Benrebiha, F.Z & Tahar, H.S. (2017): Influence of seasonal variation on chemical composition and antioxidant activity of the essential oil of field wormwood Atemisia campestris. Revue Agrobiologia 7(2): 502-511.
Belhattab, R., Boudjouref, M., Barroso, J.G., Pedro, L.P., Figueirido, A.C. (2011): Essential Oil Composition from Artemisia campestris Grown in Algeria. Advances in Environmental Biology 5(2): 429-432. ISSN 1995-0756.
Judzentiene, A., Budiene, J. (2014): Variability of Artemisia campestris L. essential oils from Lithuania. J Ess Oil Res 26: 328–333. DOI: https://doi.org/10.1080/10412905.2014.933132
Maarfia, S., Zellagui1, A., Hakki Alma, M., Göçeri, A., Karaoğul, E., Gherraf, N. (2018) Essential Oils of Bellis Sylvestris, Asteriscus Maritimus and Artemisia Campestris Stems Growing in Different Areas in Algeria. World Journal of Environmental Biosciences ISSN, 2277- 8047.
Judzentiene, A., Budiene, J., Butkiene, R., Kupcinskiene, E., Laffont-Schwobc, I., Masotti, V. (2010): Caryophyllene Oxide-rich Essential Oils of Lithuanian Artemisia campestris ssp. campestris and Their Toxicity. Nat Prod Commun 5: 1981-1984. DOI: https://doi.org/10.1177/1934578X1000501232
Ennajar, M., Bouajila, J., Lebrihi, A., Mathieu, F., Abderraba, M., Raies, A., Romdhane, M. (2009): Chemical composition and antimicrobial and antioxidant activities of essential oils and various extracts of Juniperus phoenicea L. (Cupressacees). Journal of Food Science 74(7): 364-371p. DOI: https://doi.org/10.1111/j.1750-3841.2009.01277.x
Oliva, d.l.M., Beltramino, E., Gallucci, N., Casero, C., Zygadlo, J., Demo, M. (2010): Antimicrobial activity of essential oils of Aloysia triphylla (L’Her.) Britton from different regions of Argentina. Boletin Latinoamericano y del Caribe de Plantas Mecidinales 9: 29-37p.
Viuda-Martos, M., Mohamady, M.A., Fernández-López, J., Abd El Razik, K.A., Omer, E.A., Pérez-Alvarez, J.A., Sendra, E. (2011): In vitro antioxidant and antibacterial activities of essential oils obtained from Egyptian aromatic plants. Food Control 22: 1715-1722p. DOI: https://doi.org/10.1016/j.foodcont.2011.04.003
Bertella, A. (2019): Study of the antimicrobial and antioxidant activity of the essential oils of Artemisia Herba alba, Artemisia campestris & Rosmarinus tournfortti, Doctoral Thesis, Ahmed Ben-Balla Oran University 1, Algeria.
Khebri, S. (2011): Chemical and biological study of essential oils from three Artemisia. Magister's thesis. Hadj Lakhdar Batna University, Algeria 150p.
Ultee, A., Bennik, M.H.J., Moezelaar, R. (2002): The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl Environ Microbiol 68(4): 1561-1568. DOI: https://doi.org/10.1128/AEM.68.4.1561-1568.2002
Fillippi, J.J., lanfranchi, D.A., Prado, S., Baldovini, N., Meierhenich, U.J. (2006): Composition, distribution and antibacterial activity of the essential oil Achillea ligustica. All. from crosica. J. DOI: https://doi.org/10.1021/jf060752y
Rosato, A., Piarulli, M., Corbo, F., Muraglia, M., Carone, A., Vitali, M.E., Vitali, C. (2010): In-vitro synergistic antibacterial action of certain combinations of gentamicin and essential oils. Current Medicinal Chemistry 17: 3289-3295. DOI: https://doi.org/10.2174/092986710792231996
Milenkovića, M., Stošovića, J & Slavkovskab, V. (2018): Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics. Natural Product Communications 371 – 374. DOI: https://doi.org/10.1177/1934578X1801300325
Moussaoui, F., Alaoui, T. (2016): Evaluation of antibacterial activity and synergistic effect between antibiotic and the essential oils of some medicinal plants. Asian Pac J Trop Biomed 6(1): 32–37. DOI: https://doi.org/10.1016/j.apjtb.2015.09.024
Dib, I., Fauconnier, M.L., Sindic, M., Belmekki, F., Assaidi, A., Berrabah, M., Mekhfi, H., Aziz, M., Legssyer, A., Bnouham, M and Ziyyat, A. (2017): Chemical composition, vasorelaxant, antioxidant and antiplatelet effects of essential oil of Artemisia campestris L. from Oriental Morocco. BMC Complementary and Alternative Medicine DOI 10.1186/s12906-017-1598-2. DOI: https://doi.org/10.1186/s12906-017-1598-2
Sefi, M., Fetoui, H., Makni, M., Zeghal, N. (2010): Mitigating effects of antioxidant properties of Artemisia campestris leaf extract on hyperlipidemia, advanced glycation end products and oxidative stress in alloxan-induced diabetic rats. Food Chem Toxicol 48(7):1986–93. DOI: https://doi.org/10.1016/j.fct.2010.05.005
Reanmongkol, W., Noppapan, T., Subhadhirasakul S. (2009): Antinociceptive, antipyretic, and anti-inflammatory activities of Putranjiva roxburghii Wall. leaf extract in experimental animals. Journal of natural medicines 63(3):290–296. DOI: https://doi.org/10.1007/s11418-009-0336-6
DiRosa, M., Giroud, J.P., Willoughby, D.A. (1971): Studies on the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. DOI: https://doi.org/10.1002/path.1711040103
Olajide, O.A., Makinde, M.J., Awe, S.O. (1999): Effects of the aqueous extract of Bridelia ferruginea stem bark on carrageenan-induced oedema and granuloma tissue formation in rats and mice. J Ethnopharmacol 66(1): 113-117. DOI: https://doi.org/10.1016/S0378-8741(99)00006-9
Posadas, L., Bucci, M., Roviezzo, F., Rossi, A., Parente, L., Sautebin, L., Cirino, G. (2004): Carrageenan-induced mouse paw oedema is biphasic, age-weight dependent and displays diffenrential nitric oxide cyclooxygenase-2 expression. British Journal of Pharmacology 142(2): 331-338p. DOI: https://doi.org/10.1038/sj.bjp.0705650
Gupta, M., Mazumder, U.K., Sambath Kumar, R., Gomathi, P., Rajeshwar, Y., Kakoti, B.B & Tamil Selven, V. (2005): Anti-inflammatory, analgesic and antipyretic effects of methanol extract from Bauhinia racemosa stem bark in animal models. Journal of Ethnopharmacology 98(3): 267–273. DOI: https://doi.org/10.1016/j.jep.2005.01.018
Tanas, S., Odabasoglu, F., Halici, Z., Cakir, A., Aygun, H., Aslan, A & Suleyman, H. (2010): Evaluation of anti-inflammatory and antioxidant activities of Peltigera rufescens lichen species in acute and chronic inflammation models. Journal of natural medicines 64(1): 42–49. DOI: https://doi.org/10.1007/s11418-009-0367-z
Kim, H.P., Son, K.H., Chang, H.W., kong, S.S. (2004): Anti-inflammatory plant flavonoids and cellular action macanism. J Pharmac. Sci 96: 229-254. DOI: https://doi.org/10.1254/jphs.CRJ04003X
Mujumdar, A.M & Misar, A.V. (2004): Anti-inflammatory activity of Jatropha curcas roots in mice and rats. J Ethnopharmacol 90: 11–15. DOI: https://doi.org/10.1016/j.jep.2003.09.019
Panthong, A., Kanjanapothi, D., Taesotikul, T., Phankummoon, A., Panthong, K., Reutrakul, V. (2004): Anti-inflammatory activity of methanolic extracts from Ventilago harmandiana Pierre. J Ethnopharmacol 91: 237242. DOI: https://doi.org/10.1016/j.jep.2003.12.025
González-Gallego, J., Sánchez-Campos, S., Tuñón, M.J. (2007): Nutricin Hospitalaria 22(3): 287-293.
Sayyah, M., Saroukhani, G., Peirovi, A., Kamalinejad, M. (2003): Analgesic and anti-inflammatory activity of the leaf essential oil of Laurus nobilis Linn. Phytotherapy Research 17: 733-736. DOI: https://doi.org/10.1002/ptr.1197
Chao, L.K., Hua, K.F., Hsu, H.Y., Cheng, S.S., Liu, J.Y., Chang, S.T. (2005): Study on the anti-inflammatory activity of essential oil from leaves of Cinnamomum osmophloeum. Journal of Agricultural and Food Chemistry 53: 7274-7278. DOI: https://doi.org/10.1021/jf051151u
Miguel, M.G. (2010): Antioxidant and anti-inflammatory activities of essential oils: A short review. Molecules 15: 9252-9287. DOI: https://doi.org/10.3390/molecules15129252
Ghlissi, Z., Sayari, N., Kallel, R., Bougatef, A & Sahnoun, Z. (2016): Antioxidant, antibacterial, anti-inflammatory and wound healing effects of Artemisia campestris aqueous extract in rat. Biomed. Pharm 84: 115-122. DOI: https://doi.org/10.1016/j.biopha.2016.09.018
Boudjouref, M. (2019): Antioxidant and anti-inflammatory activities of Artemisia campestris extracts, Doctoral Thesis, Ferhat Abbas Sétif University 1, Algeria.
Medila, I., Toumi, I., Ferhat, I., & Mehaych, R. (2017): Biological Evaluation of Anti-Inflammatory Activity of Artemisia campestris L. and Spitzelia coronopifolia Desf Ethanolic Leaves Extract. Journal of Chemical and Pharmaceutical Research 9(7):1-4.
Bruneton, J. (1999): Pharmacognosy, Phytochemistry, Medicinal Plants, Lavoisier Publishing. Paris 367-370.
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