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UV-Vis spectrophometer, HPTLC, HPLC, GC-MS, FTIR, consortium, metabolic activity


A detailed characterization of degraded metabolites is essential for understanding the mechanisms of complex dye degradation, as a result of metabolic activity of micro-organisms. The resulting knowledge may prove insightful for designing microbial tools for the purpose of bioremediation. In the current study, an azo dye RR120, completely decolorized by a bacterial consortium RAR, was analysed for identification of degraded metabolites. For this purpose, the dye degradation was first confirmed using qualitative techniques like UV-Vis spectrophotometer and HPTLC. On confirmation of biodegradation, the metabolites thus produced were analysed using techniques like HPLC, GC-MS and FTIR to identify stable and/ or unstable intermediate products. Specifically, these studies indicated that more than 2 intermediates are produced on degradation of RR120 dye. The cleavage of –N=N–bond, degradation of aromatic rings, loss of sulphone groups and breakage of C–Cl bond was evident from FTIR spectrum. Based on HPTLC analysis, there occurred a high probability that the degraded metabolites were assimilated by the cells of pure cultures and consortium RAR. Finally, based on above information, a detailed azo dye RR120 degradation pathway was proposed. Thus, the current study provides complete information on the metabolic activity of bacterial consortium RAR and the degradation of complex azo dye RR120.


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How to Cite

Radhika, B., & K., A. (2022). ELUCIDATING A PATHWAY FOR DEGRADATION OF AZO DYE REACTIVE RED 120 BY BACTERIAL CONSORTIUM. Journal of Applied Biological Sciences, 16(3), 396–417. Retrieved from https://jabsonline.org/index.php/jabs/article/view/1042