In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase

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Authors

  • Mahinur KIRICI
  • Muammer KIRICI
  • Şükrü BEYDEMIR
  • Metin BÜLBÜL

Keywords:

Glucose 6-phosphate dehydrogenase, Gilthead sea bream, Insecticides, Inhibition

Abstract

In this study, effects of some insecticides (cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin) on glucose 6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ oksidoreductase, EC 1.1.1.49; G6PD) from gilthead sea bream (Sparus aurata) have been investigated in vitro. In this purpose, G6PD enzyme was purified 1864.3-fold with a yield of 54.6% from fish liver using 2’,5’-ADP
Sepharose 4B affinity chromatography. Activity of the enzyme was determined by Beutler method spectrophotometrically. The purify of the enzyme was checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and the purified enzyme showed a single band on the gel. Additionally, inhibitory effects of some insecticides (cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin) on G6PD
were investigated. For this aim, IC50 values for cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin were determined by plotting activity % vs. [I]. IC50 values of cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin were 0.237, 0.016, 0.071, 0.021 and 0.187 mM, respectively. From these results, we showed that deltamethrin is the most potent inhibitor of fish liver G6PD enzyme.

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Published

2019-06-15

How to Cite

KIRICI, M., KIRICI, M., BEYDEMIR, Şükrü, & BÜLBÜL, M. (2019). In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase. Journal of Applied Biological Sciences, 10(2), 46–50. Retrieved from https://jabsonline.org/index.php/jabs/article/view/501

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