ELEMENT CONCENTRATIONS IN HORSE BLOOD AND RELATION BETWEEN AGE, GENDER, BREED, HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS
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Horse, essential elements, non-essential elements, niochemistry, hematologyAbstract
The aim of this study was to determine the concentrations of macro and essential and non-essential trace elements and correlation between biochemical and hematological parameters, age, gender, and breed in horses. The whole blood samples of 20 horses were collected from İstanbul (n=11) and Tekirdağ (n=9) cities. Macro (Ca, K, Mg, and P), trace essential (Cu, Fe, Se, and Zn) and non-essential (Al, Cd, Hg, and Pb) element concentrations were determined by ICP-OES. In addition, hematological and biochemical parameters levels were determined. Ca, K and Mg concentrations were within the normal reference range, P concentrations were higher than the reference in horses. Blood concentrations were sorted as K>Ca>P>Mg for macro (mg L-1) elements, were Fe>Cu> Zn>Se for essential trace (μg L-1) elements and as Al>Pb>Cd>Hg for non-essential trace (μg L-1) elements. The negative and positive correlation between the hematological and biochemistry parameter levels and element levels were significant (p <0.05, p <0.01) between Mg-TRIG, Mg-CHOL, Zn-TB, Al-LDL, and Hg-CREA.
References
Yarsan, E., Yipel, M., Dikmen, B., Altıntaş, L., Ekici, H., Köksal, A. (2014): Concentrations of essential and non-essential toxic trace elements in wild boar (Sus Scrofa L., 1758) tissues from southern Turkey. Bulletin of Environmental Contamination and Toxicology 92(1):10-14.
Fazio, F., Gugliandolo, E., Nava, V., Piccione, G., Giannetto, C., & Licata, P. (2020): Bioaccumulation of Mineral Elements in Different Biological Substrates of Athletic Horse from Messina, Italy. Animals 10(10): 1877.
Rayner-Canham, G. (2021): The Periodic Table: Past, Present, and Future. Chemistry International.
NRC (2005): National Research Council. Mineral tolerance of animals, 2nd ed., The National Academies Press. USA
Yarsan, E., Yipel, M., Altınok Yipel, F., Dikmen, B. (2014): Accumulation of nonessential potentially toxic trace elements (PTEs) in the some economically important seafood species of Mediterranean. Kafkas University Veterinary Journal 20 (2): 185-188.
Khaneghah, A. M., Fakhri, Y., Nematollahi, A., Pirhadi, M. (2020): Potentially toxic elements (PTEs) in cereal-based foods: a systematic review and meta-analysis. Trends in Food Science & Technology 96: 30-44.
Yipel, M., Tekeli, İ.O., Dikmen, B., Yarsan, E. (2021): Distribution and Ecotoxicological Risk Assessment of Heavy Metals in Streams of Amanos Mountains from Southern Turkey. Bulletin of Environmental Contamination and Toxicology 107(5): 895-903.
Gupta, R.C. (2012): Veterinary toxicology: Basic and Clinical Principles, 2nd ed., Academic press. USA.
Bampidis, V.A., Nistor, E., Nitas, D. (2013): Arsenic, cadmium, lead and mercury as undesirable substances in animal feeds. J Anim Sci Biotechnol 46(1):17-22.
López-Alonso, M. (2012): Animal feed contamination by toxic metals. In: Fink-Gremmels J (ed) Animal Feed Contamination. Woodhead Publishing, Philadelphia, pp. 19102-3406, USA.
Moeller, R.B., Albretsen, J.C., Coppock, R., Dziwenka, M.M., Ensley, S., Gwaltney-Brant, S., (2004): Metals and Minerals. In: Plumbee K. (ed) Clinical Veterinary Toxicology. Mosby, Philadelphia, USA.
Yipel, M., & Yarsan, E. (2014): A risk assessment of heavy metal concentrations in fish and an invertebrate from the Gulf of Antalya. Bulletin of Environmental Contamination and Toxicology 93(5): 542-548.
Massanyi, P., Stawarz, R., Halo, M., Formicki, G., Lukac, N., Cupka, P., Kovacik, J. (2014): Blood concentration of copper, cadmium, zinc and lead in horses and its relation to hematological and biochemical parameters. Journal of Environmental Science and Health Part A 49(8): 973-979.
Ghorbani, A., Mohit, A., Kuhi, H. D. (2015): Effects of dietary mineral intake on hair and serum mineral contents of horses. Journal of Equine Veterinary Science 35(4): 295-300.
Raghu, V. (2015): Study of dung, urine, and milk of selected grazing animals as bioindicators in environmental geoscience—a case study from Mangampeta barite mining area, Kadapa District, Andhra Pradesh, India. Environmental Monitoring and Assessment 187(1):1-10.
Tripodi, M. A., Muschetto, E., Cueto, G., Hancke, D., & Suárez, O. (2020): Are soft tissues of urban rats good indicators of exposure to heavy metal pollution? A study conducted in one of the most polluted basins of Buenos Aires, Argentina. Environmental Monitoring & Assessment 192(6):1-11.
Brummer-Holder, M., Cassill, B. D., Hayes, S. H. (2020): Interrelationships between age and trace element concentration in horse mane hair and whole blood. Journal of Equine Veterinary Science 87: 102922.
Komarova, T., McKeating, D., Perkins, A. V., & Tinggi, U. (2021): Trace Element Analysis in Whole Blood and Plasma for Reference Levels in a Selected Queensland Population, Australia. International Journal of Environmental Research and Public Health, 18(5): 2652.
Maia, L., de Souza, M. V., Fernandes, R. B. A., Fontes, M. P. F., de Souza Vianna, M. W., Luz, W. V. (2006): Heavy metals in horse blood, serum, and feed in Minas Gerais, Brazil. Journal of Equine Veterinary Science 26(12): 578-583.
Zaccaroni, A., Corteggio, A., Altamura, G., Silvi, M., Di Vaia, R., Formigaro, C., Borzacchiello, G. (2014): Elements levels in dogs from “triangle of death” and different areas of Campania region (Italy). Chemosphere 108: 62-69.
Squadrone, S., Brizio, P., Stella, C., Mantia, M., Favaro, L., Biancani, B., Abete, M. C. (2019): Differential bioaccumulation of trace elements and rare earth elements in the muscle, kidneys, and liver of the invasive Indo-Pacific Lionfish (Pterois spp.) from Cuba. Biological Trace Element Research 196(1): 1-10.
Yao, T., Zhu, G., Zhang, Y., Yan, P., Li, C., & de Boer, W. F. (2021): Bird's feather as an effective bioindicator for detection of trace elements in polymetallic contaminated areas in Anhui Province, China. Science of the Total Environment 771: 144816.
Shawaf, T., Al Bulushi, S., Al-Ali, M. A., Meligy, A. M. A., Salouci, M., & Hussen, J. (2021): Investigation of some trace elements and hematological and biochemical parameters in the blood of emaciated Omani goats. Veterinary World 14(7): 1960.
Laur, N., Kinscherf, R., Pomytkin, K., Kaiser, L., Knes, O., & Deigner, H. P. (2020): ICP-MS trace element analysis in serum and whole blood. PloS One 15(5): e0233357.
Baptista, T., Redígolo, M., Zamboni, C., Sato, I., & Marcelino, J. (2012): Comparative study of inorganic elements determination in whole blood from Crioula breed horse by EDXRF and NAA analytical techniques. Journal of Radioanalytical and Nuclear Chemistry 291(2): 399-403.
Goullé, J.P., Mahieu, L., Castermant, J., Neveu, N., Bonneau, L., Lainé, G., Bouige, D., Lacroix, C. (2005): Metal and metalloid multi-elementary ICP-MS validation in whole blood, plasma, urine and hair. Reference concentrations. Forensic Sci Int 153(1):39–44.
Cedeño, Y., Miranda, M., Orjales, I., Herrero-Latorre, C., Suárez, M., Luna, D., López Alonso, M. 2020: Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs. Animals 2020(10): 1052.
Sprayberry, K. A., & Robinson, N. E. (2014): Robinson's Current Therapy in Equine Medicine-E-Book. Elsevier Health Sciences.
Lumsden, J. H., Rowe, R., & Mullen, K. (1980): Hematology and biochemistry reference concentrations for the light horse. Canadian Journal of Comparative Medicine 44(1): 32.
Toribio, R. E. (2011): Disorders of calcium and phosphate metabolism in horses. Veterinary Clinics: Equine Practice 27(1): 129-147.
Asirvatham, J. R., Moses, V., & Bjornson, L. (2013): Errors in potassium measurement: a laboratory perspective for the clinician. North American Journal of Medical Sciences 5(4): 255.
Cornes, M. P., Ford, C., & Gama, R. (2008): Spurious hyperkalaemia due to EDTA contamination: common and not always easy to identify. Annals of Clinical Biochemistry 45(6): 601-603.
Hawkins R. (2003): Measurement of whole-blood potassium: Is it clinically safe? Clin Chem. 49(12): 2105–2106.
Ghaffar, A., Hussain, R., Khan, A., Abbas, R., Asad, M. (2015): Clinico-Hematologıical and mutagenic changes induced by arsenic and copper sul- phate in adult poultry males. J. Anim. Plant Sci 25 (6): 1555-61.
Altinok-Yipel, F., Yipel, M., Altuğ, N., & Özdemir, N. (2022): Blood concentrations of potentially toxic trace elements (PTEs) and correlation with biochemical and hematological parameters in dogs from thrace region, Turkey. Chemosphere 133649.
Andjelkovic, M., Buha Djordjevic, A., Antonijevic, E., Antonijevic, B., Stanic, M., Kotur-Stevuljevic, J., ... & Bulat, Z. (2019): Toxic effect of acute cadmium and lead exposure in rat blood, liver, and kidney. J. Environ. Res. Public Health. 16(2): 274.
Yousef, M., Ibrahim, H., Yacout, H., Hassan, A. (1998): Ef- fects of cypermethrin and dimethoate on some physiological and biochemical parameters in barki sheep. Egyptian J Nutr Feeds 1(1): 41-52.
Peters, J. L., Perry, M. J., McNeely, E., Wright, R. O., Heiger-Bernays, W., & Weuve, J. (2021): The association of cadmium and lead exposures with red cell distribution width. PloS One 16(1): e0245173.
Badiei, K., Mostaghni, K., Nikghadam, P., & Poorjafar, M. (2010): The effect of mercury on thyroid function in sheep. Iran J Vet Med 4(4): 277-281.
Shawaf, T., Almathen, F., Meligy, A., El-Deeb, W., & Al-Bulushi, S. (2017): Biochemical analysis of some serum trace elements in donkeys and horses in Eastern region of Kingdom of Saudi Arabia. Veterinary World 10(10): 1269.
Asano, R., Suzuki, K., Otsuka, T., Otsuka, M., Sakurai, H. (2002): Concentrations of toxic metals and essential minerals in the mane hair of healthy racing horses and their relation to age. J Vet Med Sci 64: 607-610.
Rudy, M. (2009): Correlation of lead, cadmium and mercury levels in tissue and liver samples with age of cattle. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 26(6): 847-853.
Nazifi, S., Eskandarzade, N., Khosravi, M., Haddadi, M., & Rahsepar, M. (2017): Serum Concentrations of Strontium, Lead, Nickel, Vanadium and Aluminum in Horses. J Vet Sci Technol, 8(457): 2.
De Souza, M.V., Ferreira Fontes, M.P., Alves Fernandes, R.B. (2014): Heavy metals in equine biological components. R Bras Zootec 43: 60-66.
Southwood, L. L. (Ed.). (2012): Practical guide to equine colic. John Wiley & Sons.
Manual, M. V. (2016): Overview of Enteric Campylobacteriosis. Kenilworth (NJ): Merck Sharp & Co. https://www.merckvetmanual.com/multimedia/table/v3362735. Erişim tarihi: 12.09.2021.
Kaneko, J. J., Harvey, J. W., & Bruss, M. L. (Eds.). (2008): Clinical Biochemistry of Domestic Animals. Academic press.
Knottenbelt, D. C., & Malalana, F. (2014): Saunders Equine Formulary E-Book. Elsevier Health Sciences.
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