INVESTIGATION OF THE CYTOTOXIC EFFECTS OF LAVANDULA STOECHAS ESSENTIAL OILS IN PANCREATIC DUCTAL ADENOCARCINOMA
Abstract views: 23 / PDF downloads: 117Keywords:
Pancreatic ductal adenocarcinoma, L. stoechas, cytotoxicity, K-ras, EGFRAbstract
Pancreatic cancer is one of the highest mortality types in the world and it is reported that it ranks 4th in the latest data. In recent years, studies on the treatment of the disease has focused on active substances derived from natural products. Lavender plant is known to have many biological activities including cytotoxic effects in many cancer studies. The aim of this study is to investigate the cytotoxic efficacy of essential oils of Lavandula stoechas (L. stoechas) extracted by water distillation in pancreatic ductal adenocarcinoma. Pancreatic adenocarcinoma cells (AsPC-1) were produced on high glucose DMEM medium and separated into control and L. stoechas essential oil applied group (LS). L. stoechas essential oil was obtained by water distillation method and applied to cells at different concentrations (0.1, 0.25, 0.5 and 1 µg/ml) for 24 hours. Cell viability tests were performed by MTT method and effective concentration was determined. Expression levels of EGFR and K-ras oncogenes were investigated by real-time quantitative polymerase chain reaction from mRNA samples. Viability tests showed that LS has the effective concentration of 1 µg/ml concentration. In gene expression analysis, it was found that effective concentration of LS downregulates the expression of both EGFR and Kras oncogenes. In the light of all these data, it was concluded that essential oil components of L. stoechas could be considered as a potential agent in the regression of PDAK pathogenesis. In order to be able to say this subject in full, in vivo and further studies are needed.
References
Yokuş, B., Çakır, Ü.D. (2012): Kanser Biyokimyası. Dicle Üniv Vet Fak Dergisi 1(2): 7-18.
İzgi, K. Kanser Biyokimyası. In: Paşaoğlu H, editor. Temel/Klinik Biyokimya. 1.Baskı Ankara: Pelikan Kitabevi 2017; pp:821-831.
Özgenoğlu, S., Aydoğdu, G., Dinçsoy, A.B., Taghidizaj, A.A., Derici, K., Yılmaz, E., Aras, S., Duman, C.D. (2013): Liken Sekonder Bileşiklerinin Farklı İnsan Kanser Hücre Tipleri Üzerine Anti-kanserojenik Etkisi. Turk Hijyen ve Deneysel Biyoloji Dergisi 70(4): 215-226.
Li, W., Song, X., Yu, H., Zhang, M., Li, F., Cao, C., Jiang, Q. (2018): Dendritik Cell-Based Cancer Immunotherapy for Pancreatic Cancer. Arab Journal of Gastroenterology 19:1-6.
Magliano, M.P., Logsdon, C.D. (2013): Roles for KRAS in Pancreatic Tumor Development and Progression. Gastroenterology 144(6): 1220–1229.
Caldas, C., Kern, S.E. (1995): K-ras mutation and pancreatic adenocarcinoma. Int J Pancreatol 18(1): 1-6.
Korc, M. (1998): Role of growth factors in pancreatic cancer. Surg Oncol Clin North America 7: 25-41.
Goren, A., Topçu, G., Bilsel, G., Bilsel, M., Aydoğmuş, Z., Pezzuto, J.M. (2002): The chemical constituents and biological activity of Lavandula stocheas ssp. stocheas. Z Naturforsch 57: 797-800.
Hajhashemi, V., Ghannadi, A., Sharif, B. (2003): Anti- inflammatory properties of the leaf extracts and essential oil of Lavandula angustifolia Mill. J Ethnopharmacol 89: 67-71.
Zu, Y., Yu, H., Liang, L., Fu, Y., Efferth, T., Liu, X., Wu, N. (2010): Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules 15(5): 3200-10.
Zamanian-Azodi, M., Rezaie-Tavirani, M., Heydari-Kashal, S., Kalantari, S., Dailian, S., Zali, H. (2012): Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract. Gastroenterol Hepatol Bed Bench 5(1): 35-42.
Ali, M.A., Abul Farah, M., A”l-Hemaid, F.M., Abou-Tarboush, F.M. (2014): In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells. Genet Mol Res 13(2):3981-90.
Ayanoğlu, F., Mert, A., Kaya, A. (2000): Hatay Florasında Yetişen Karabaş Lavantanın (Lavandula stoechas subsp. stoechas L.) Çelikle Köklendirilmesi Üzerine Farklı Lokasyonların ve Hormon Dozlarının Etkisi. Turk J Agric 24: 607-610.
Özturk, B., Konyalıoğlu, S., Kantarcı, G., Cetinkol, D. (2005): İzmir Yöresindeki Yabani Lavandula stoecas L. Subsp Stoecas Taksonundan Elde Edilen Uçucu Yağın Bileşimi, Antibakteriyel, Antifungal ve Antioksidan Kapasitesi. Anadolu Ege Tarımsal Araştırma Dergisi 15(1): 61-72.
Kucukgul, A., Erdogan, S. (2014): Caffeic acid phenethyl ester (CAPE) protects lung epithelial cells against H2O2-induced inflammation and oxidative stress. HealthMED 8(3): 329-338.
McGimpsey, J.A., Porter, N.G. (1999): Lavender: A Grover’s Guide for Commersial Production. New Zeland Institute for Crop &Food Research Ltd New Zeland.
Cavanagh, H.M., Wilkinson, J.M. (2002): Biological activities of lavender essential oil. Phytother Res 16(4): 301-8.
Önel, S.E., Aksu, T., Kalamak, A., Durmuş, A.K., Aksu, D.S., Sakin, F., Türkmen, M. (2021): Effect of Some Essential Oils on in vitro Ruminal Fermentation of Alfalfa Hay. Progress in Nutrition 23(2): e2021250.
Nelson, N.J. (1997): Scents or nonsense: Aromatherapy's benefits still subject to debate. J Natl Cancer Inst 89: 1334–1336.
Perrucci, S. (1995): Acaricidal activity of some essential oils and their constituents against Tyrophagus longior, a mite of stored food. J Food 58: 560–563.
Antonov, A., Stewart, A., Walter, M. (1997): Inhibition of conidium germination and mycelial growth of Botrytis cinerea by natural products. Proceedings of the 1997 New Zealand Plant Physiology Society Conference. http://www.hortnet.-co.nz/publications/nzpps/proceedings/97/97_159.html.
Nelson, R.R.S. (1997): In vitro activities of five plant essential oils against methacillin‐resistant Staphylococcus aureus and vancomycin‐resistant Enterococcus faecium. J Antimicrob Chemother 40: 305–306.
Selmi, S., Jallouli, M., Gharbi, N., Marzouki, L. (2015): Hepatoprotective and Renoprotective Effects of Lavender (Lavandula stoechas L.) Essential Oils Against Malathion-Induced Oxidative Stress in Young Male Mice. J Med Food 18(10): 1103-11.
Shapira, S., Pleban, S., Kazanov, D., Tirosh, P., Arber N. (2016): Terpinen-4-ol: A Novel and Promising Therapeutic Agent for Human Gastrointestinal Cancers. PLoS ONE 11(6): e0156540.
Wu, C.S., Chen, J., Chen, J.W., Shieh, J., Huang, C.H., Lin, P.S., Chang, G.C., Chang, J., Lin, C. (2012): Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine Article ID 818261, 13 pages.
Nakayama, K., Murata, S., Ito, H., Iwasaki, K., Villareal, M.O., Zheng, Y.W., Matsui, H., Isoda, H., Ohkohchi, N. (2017): Terpinen-4-ol inhibits colorectal cancer growth via reactive oxygen species. Oncol Lett 14(2): 2015-2024.
Jardak, M., Elloumi-Mseddi, J., Aifa, S., Mnif, S. (2017): Chemical composition, anti-biofilm activity and potential cytotoxic effect on cancer cells of Rosmarinus officinalis L. essential oil from Tunisia Lipids Health Dis 16(1): 190.
Moteki, H., Hibasami, H., Yamada, Y., Katsuzaki, H., Imai, K., Komiya, T. (2002): Specific induction of apoptosis by 1,8-cineole in two human leukemia cell lines, but not a in human stomach cancer cell line. Oncol Rep 9(4): 757-60.
Murata, S., Risa, S., Kosugi, C., Tezuka, T., Yamazaki, M., Hirano, A., Yoshimura, Y., Suzuki, M., Shuto, K., Ohkohchi, N., Koda, K. (2013): Antitumor effect of 1, 8-cineole against colon cancer. Oncol Rep 30(6): 2647-52.
Kern, S.E. (2000): Molecular genetic alterations in ductal pancreatic adenocarcinomas. Med Clin North Am 84: 691–5.
Neesse, A., Michl, P., Frese, K.K., Feig, C., Cook, N. (2011): Stromal biology and therapy in pancreatic cancer. Gut 60: 861–8.
Rowland-Goldsmith, M.A., Maruyama, H., Matsuda, K., Idezawa, T., Ralli, M., Ralli, S., Korc, M. (2002): Soluble type II transforming growth factor-beta receptor attenuates expression of metastasis-associated genes and suppresses pancreatic cancer cell metastasis. Mol Cancer Ther 1(3): 161-7.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Journal of Applied Biological Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
JABS is licensed under a 