INVESTIGATION OF THE CYTOTOXIC EFFECTS OF LAVANDULA STOECHAS ESSENTIAL OILS IN PANCREATIC DUCTAL ADENOCARCINOMA
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Pancreatic ductal adenocarcinoma, L. stoechas, cytotoxicity, K-ras, EGFRAbstract
Pancreatic cancer is one of the highest mortality types in the world and it is reported that it ranks 4th in the latest data. In recent years, studies on the treatment of the disease has focused on active substances derived from natural products. Lavender plant is known to have many biological activities including cytotoxic effects in many cancer studies. The aim of this study is to investigate the cytotoxic efficacy of essential oils of Lavandula stoechas (L. stoechas) extracted by water distillation in pancreatic ductal adenocarcinoma. Pancreatic adenocarcinoma cells (AsPC-1) were produced on high glucose DMEM medium and separated into control and L. stoechas essential oil applied group (LS). L. stoechas essential oil was obtained by water distillation method and applied to cells at different concentrations (0.1, 0.25, 0.5 and 1 µg/ml) for 24 hours. Cell viability tests were performed by MTT method and effective concentration was determined. Expression levels of EGFR and K-ras oncogenes were investigated by real-time quantitative polymerase chain reaction from mRNA samples. Viability tests showed that LS has the effective concentration of 1 µg/ml concentration. In gene expression analysis, it was found that effective concentration of LS downregulates the expression of both EGFR and Kras oncogenes. In the light of all these data, it was concluded that essential oil components of L. stoechas could be considered as a potential agent in the regression of PDAK pathogenesis. In order to be able to say this subject in full, in vivo and further studies are needed.
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