In Vitro Effectiveness of Alcohol Based Hand and Skin Disinfectants Against Various Microorganisms

Abstract

In this study, in order to define how effective alcohol based hand and skin disinfectant is to which concentration against Staphilococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 23853), Candida albicans (ATCC 10231) strains used for Test microorganisms, final microorganisms was disinfectant into the different concentrations of the disinfectant to be tested by being prepared as 2-5x109 CFU/ml according to the McFarland 5 turbidity of test disinfectant. After disinfectants were activated with microorganisms at previously experimented 30 seconds; 1,5,15 and 30 minute periods, colony counts at 1 ml. Levels were performed by way of cast-cultural plaque metod. Consequently, it was determined that 100% concentration of alcohol based skin and hand disinfectant was effective against Staphilococcus aureus in 1 minute, while other test microorganisms were effective in 30 seconds.