Initial Determination of DNA Polymorphism of Some Primula veris L. Populations from Kosovo and Austria

Abstract

Primula veris L. is a well known pharmaceutical plant, what for it is widely collected in all SE Europe, particularly in Kosovo. Our aim
is to determine a molecular polymorphism of its populations from Kosovo. Leaves extracted DNA was investigated in details for presence of
polymorphism. RAPD analyses were conducted using 20 different short primers. Genomic DNA amplification profiles were analyzed and
processed using data labelling. Comparison between cowslip populations in genetic composition revealed that samples from Bogaj were too
distinct on their own. Analyzed molecular variation was observed to have more molecular variation within populations (73%), compared to
among populations (27%). Highest genetic distance is observed between Leqinat and M. e Madhe. He values were highest in Bogaj
population, while lowest in M. e Madhe population. Results indicated that Bogaj population are more polymorphic. RAPD allows identifying
and assessing genetic similarities among plant populations.