Production of Cold Active Lipase from Bacillus sp.

Abstract

A cold active lipase producing Bacillus sp. strains were isolated from sewage of oil. Bacillus sp. strain SY-7 was determined as the best lipase producing isolate. The highest enzyme production was found at 20°C and pH 8.0 on tributyrin media. Analyses
of molecular mass of the partial purified lipase was carried out by SDS-PAGE which revealed a single band as 110.5 kDa. The enzyme activity and stability were determined by spectrophotometric and titrimetric methods. The enzyme was active between
pH 4.0-10.0 and 5-50°C and showed optimal activity and stability at pH 8.0 and 20°C. In the presence of BaCl2 (4mM), KCl (4mM), AgNO3 (4mM), CuSO4 (4mM), MgCl2 (4mM), CaCl2 (4mM), ZnCl2 (4mM) and NaCl (4mM), the enzyme exhibited the
following activities 105%, 100%, 100%, 89%, 105%, 95%, 100% and 100%, respectively. In the presence of Tween-20(5%), Tween-80(5%), detergent-1(5%) and detergent-6(5%) the enzyme saved its original activity. SDS(5%), detergent-2(5%) and detergent-7(5%) increased the activity 10%, 5% and 5%, respectively. Detergent-3(5%), detergent-4(5%) and detergent-5(5%) reduced its activity respectively, 14%, 14% and 52%. According to these results, SY-7 lipase shows alkaline, psychrotrophilic, cold active and stable, chelator and detergent resistant properties. Owing to these properties, this lipase can be useful in detergent industry.