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Osmotic dehydratation, Antioxidant properties, Functional food, Bioactive content, Acacetin


The current research looks to benefit from Calamintha nepeta (L.) Savi. in food enrichment by using osmotic dehydratation (OD) for supplementing a solid foodstuff model. The kinetic gain of food model in terms of individual phenolics using HPLC-DAD, total phenolics (TPC), total flavonoids contents (TFC) as well as antioxidant properties were examined at different impregnation times. The food model gained the same phenolics profile of osmotic solution dominated by acacetin and Cryptochlorogenic acid. The enrichment process created a rich osmo-dehydrated food in TPC and TFC reaching the bioactive features of most of rich-in-phenolics human foods. The food model exhibited a potent antioxidant activity, even at a short impregnation time (4h), attaining 92.07±3.63% as β-carotene bleaching potency and 47.64±0.59% (at 10 mg/mL) as DPPH scavenging capacity. The kinetic gain of individual phenolics compounds depends on their concentration. This investigation supports valorization of Calamintha nepeta for the production of functional foods.


Božović, M., Ragno, R. (2017): Calamintha nepeta (L.) Savi and its Main Essential Oil Constituent Pulegone: Biological Activities and Chemistry–A review. Molecules 22: article 290.

Briskin, D. P. (2000): Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health. Plant Physiology 124: 507–514.

McDougall, G. J. (2017): Phenolic-enriched foods: sources and processing for enhanced health benefits. Proceedings of the Nutrition Society 76(2): 163–171.

Davies K. (2000): Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. International Union of Biochemistry and Molecular Biology-Life 50(4–5): 279–289.

Gonçalves, S., Moreira, E., Grosso, C., Andrade, P. B., Valentão, P., Romano, A. (2016): Phenolic profile, antioxidant activity and enzyme inhibitory activities of extracts from aromatic plants used in Mediterranean diet. Journal of Food Science and Technology 54(1): 219–227.

Merouane, A., Saadi, A., Noui, A. (2018): Impact of removal of micro and Nano sized particles on the phenolic content and antioxidant activity: Study on aqueous and methanolic leaves extracts of Phlomis crinita. Industrial Crops and Products 114: 132–136.

Costa, D. C., Costa, H. S., Albuquerque, T. G., Ramos, F., Castilho, M. C., Sanches-Silva, A. (2015): Advances in phenolic compounds analysis of aromatic plants and their potential applications. Trends in Food Science and Technology 45: 336–354.

Phoon, K. Y., Ng, H. S., Zakaria, R., Yim, H. S., Mokhtar, M. N. (2018): Enrichment of minor components from crude palm oil and palm-pressed mesocarp fibre oil via sequential adsorption-desorption strategy. Industrial Crops and Products 113: 187–195.

Olson, R., Gavin-Smith, B., Ferraboschi, C., Kraemer, K. (2021): Food Fortification: The Advantages, Disadvantages and Lessons from Sight and Life Programs-review-. Nutrients 13: e1118.

Bourekoua, H., Różyło, R., Gawlik‑Dziki, U., Zidoune, N. M., Dziki, D. (2018): Evaluation of physical, sensorial, and antioxidant properties of gluten‑free bread enriched with Moringa oleifera leaf powder. European Food Research and Technology 244: 189–195.

Alenisan, M. A., Alqattan, H. H., Tolbah, L. S., Shori, A. B. (2020): Antioxidant properties of dairy products fortified with natural additives: A review. Journal of the Association of Arab Universities for Basic and Applied Sciences 24: 101–106.

Rózek, A., Achaerandio, I., Almajano, M. P., Güell, C., López, F., Ferrando, M. (2007): Solid Foodstuff Supplemented with Phenolics from Grape: Antioxidant Properties and Correlation with Phenolic Profiles. Journal of Agricultural and Food Chemistry 55: 5147–5155.

Rastogi, N. K., Raghavarao, K. S. M. S., Niranjan, K. (2005): Developments in Osmotic Dehydration. In: D-W. Sun (ed.) Emerging Technologies for Food Processing, 1st edition (pp. 221-249), Academic Press, San Diego, California, USA.

Bchir, B., Besbes, S., Attia, H., Blecker, C. (2012): Osmotic dehydration of pomegranate seeds (Punica granatum L.): Effect of freezing pretreatment. Journal of Food Process Engineering 35(3): 335– 354.

Beristatn, C. I., Azuara, E., Cortés, R., Garcia, H. S. (1990): Mass transfer during osmotic dehydration of pineapple rings. Journal of Food Science and Technology 25: 576–582.

Singleton, V. L., Orthofer, R., Lamuela-Raventoís, R. M. (1999): Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent (pp.152-178). In: L. Packer (ed.) Methods in enzymology: oxidants and antioxidants (volume 299), Academic Press, San Diego, California, USA.

Tepe, B., Degerli, S., Arslan, S., Malatyali, E., Sarikurkcu, C. (2011): Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucriumpolium and Stachys iberica. Fitoterapia 82: 237–246.

Muid, S., Ali, A. M., Yusoff, K., Nawawi, H. (2013): Optimal antioxidant activity with moderate concentrations of Tocotrienol rich fraction (TRF) in in vitro assays. International Food Research Journal 20(2): 687–694.

Dapkevicius, A., Venskutonis, R., Van Beek, T. A., Linssen, P. H. (1998): Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture 77: 140–146.<140::AID-JSFA18>3.0.CO;2-K

Sadilova, E., Carle, R., Stintzing, F. C. (2007): Thermal degradation of anthocyanins and its impact on color and in vitro antioxidant capacity. Molecular Nutrition and Food Research 51: 1461–1471.

Marin, P. D., Grayer, R. J., Veitch, N. C., Kite, G. C., Harborne, J. B. (2001): Acacetin glycosides as taxonomic markers in Calamintha and Micromeria. Phytochemistry 58: 943–947.

Pacifico, P., Galasso, S., Piccolella, S., Kretschmer, N., Pan, S-P., Marciano, S., Bauer, R., Monaco, P. (2015): Seasonal variation in phenolic composition and antioxidant and anti-inflammatory activities of Calamintha nepeta (L.) Savi. Food Research International 69: 121–132. https://doi.org10.1016/j.foodres.2014.12.019

Janicsák, G., Máthé, I., Miklóssy-Vári, V., Blunden, G. (1999): Comparative studies of the rosmarinic and caffeic acid contents of Lamiaceae species. Biochemical Systematics and Ecology 27: 733–738.

Singh, S., Gupta, P., Meena, A., Luqman, S. (2020): Acacetin, a flavone with diverse therapeutic potential in cancer, inflammation, infections and other metabolic disorders. Food and Chemical Toxicology 145: 111708.

Ma, L., Tang, L., Yi, Q. (2019): Salvianolic Acids: Potential Source of Natural Drugs for the Treatment of Fibrosis Disease and Cancer. Frontiers in Pharmacology 10: 97.

Nunes, S., Madureira, R., Campos, D., Sarmento, B., Gomes, A. M., Pintado, M., Reis, F. (2017): Therapeutic and Nutraceutical Potential of Rosmarinic Acid—Cytoprotective Properties and Pharmacokinetic Profile. Critical Reviews in Food Science and Nutrition: 57(59): 1799-1806.

Das, J., Thapa, S., Pradhan, D., Thorat, S. S., Talukdar, N. C. (2013): Intra-specific genetic diversity, phytochemical analysis and antioxidant activities of a potential Himalayan Swertia (Swertia bimaculata Hook.F. and Thomas.). Industrial Crops and Products 49: 341–347.

Ferrali, M., Signorini, C., Caciotti, B., Sugherini, L., Ciccoli, L., Giachetti, D., Comporti, M. (1997): Protection against oxidative damage of erythrocyte membrane by the flavonoid quercetin and its relation to iron chelating activity. Federation of European Biochemical Societies Letters 416: 123–129.

Elliott, A. J., Scheiber, S. A., Thomas, C., Pardini, R. S. (1992): Inhibition of glutathione reductase by flavonoids. A structure-activity study. Biochemical Pharmacology 44: 1603–1608.

Cos, P., Ying, L., Calomme, M., Hu, J. P., Cimanga, K., Van Poel, B., Pieters, L., Vlietinck, A. J., VandenBerghe, D. (1998): Structure-activity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers. Journal of Natural Products 61: 71–76.

Ovaskainen, M. L., Törrönen, R., Koponen, J. M., Sinkko, H., Hellström, J., Reinivuo, H., Mattila, P. (2008): Dietary intake and major food sources of polyphenols in Finnish adults. Journal of Nutrition 138: 562–566.

Marinova, D., Ribarova, F., Atanassova, M. (2005): Total phenolics and total flavonoids Inbulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy 40(3): 255–260.

Recuenco, M. C., Lacsamana, M. S., Hurtada, W. A., Sabularse, V. C. (2016): Total Phenolic and Total Flavonoid Contents of Selected Fruits in the Philippines. Philippine Journal of Science 145(3): 275–281.

Ali Haimoud, S., Allem, R., Merouane, A. (2016): Antioxidant and anti-inflammatory properties of widely consumed date palm (Phoenix dactylifera L.) Fruit varieties in Algerian oases. Journal of Food Biochemistry 40: 463–471.

USDA. (2015): USDA Database on the Procyanidin Content of Selected Foods, release 2, Nutrient Data Laboratory, Beltsville Human Nutrition Research Center, ARS, USDA.

Sicari, V., Pellicanò, T. M., Giuffrè, A. M., Zappia, C., Capocasale, M. (2016): Bioactive compounds and antioxidant activity of citrus juices produced from varieties cultivated in Calabria. Journal of Food Measurement and Characterization 10: 773–780.




How to Cite

Merouane, A., Fellag, S., & Noui, A. (2022). CREATION OF FUNCTIONAL SOLID FOOD MODEL ENRICHED WITH CALAMINTHA NEPETA (L.) SAVI.: KINETIC GAIN OF PHENOLICS COMPOUND. Journal of Applied Biological Sciences, 16(2), 326–339. Retrieved from