EFFECTS OF MEDIUM SUPPLEMENTS ON VIABILITY AND FUNCTIONALITY IN LONG-TERM CULTURED ISLET CELLS

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Keywords:

Bovine serum albumin, fetal bovine serum, islet, insulin-transferrin-sodium selenite premix, viability

Abstract

It is highly important to keep islet cells alive and functional before transplantation. This study aims to maintain the viability and functionality of islets with different supplements and provide longer incubation. Isolated islets were cultured with RPMI1640 with 10% fetal bovine serum (FBS) or 1% insulin-transferrin-sodium selenite (ITS) premix or 0.3% bovine serum albumin (BSA) up to 120h. Islets were evaluated in terms of viability, functionality and morphologically. Islet viability of the BSA group decreased significantly in 72, 96, and 120 h (P < 0.05) compared to FBS and ITS groups. While insulin secretion of the BSA group was lower than the others at only 48 and 72 h, the stimulation index was at rational reasonable for transplantation in all groups at 48h and only in the FBS and ITS groups at 72 h. However, islet cells adhere to each other in the FBS group after 48h. Moreover, total oxidant status was the highest and total antioxidant status was the lowest in the BSA group at 120 h. It was concluded that ITS premix could be preferred to FBS and BSA in long-term islet cultures.

References

Van der Valk, J., Brunner, D., De Smet, K., Svenningsen, A.A., Honegger, p., Knudsen, L.E., Lindl, T., Noraberg, J., Price, A., Scarino, M.L. (2010): Optimization of Chemically Defined Cell Culture Media–Replacing Fetal Bovine Serum in Mammalian In Vitro Methods. Toxicology In Vitro. 24: 1053–1063.

Gstraunthaler, G. (2003): Alternatives to The Use of Fetal Bovine Serum: Serum-Free Cell Culture. ALTEX-Alternatives to Animal Experimentation 20: 275–281.

Taub, M. (1990): The Use of Defined Media in Cell and Tissue Culture. Toxicology In Vitro 4: 213–225.

Nacher, M., Estil·Les, E., Garcia, A., Nadal, B., Pairó, M., Garcia, C., Secanella, L., Montanya, E. (2016): Human Serum Versus Human Serum Albumin Supplementation in Human Islet Pretransplantation Culture: In Vitro and in Vivo Assessment. Cell Transplantation 25: 343–352.

Daoud, J.T., Petropavlovskaia, M. S., Patapas, J.M., Degrandpré, C.E., DiRaddo, R.W., Rosenberg, L., Tabrizian, M. (2011): Long-Term In Vitro Human Pancreatic Islet Culture Using Three-Dimensional Microfabricated Scaffolds. Biomaterials 32: 1536–1542.

Boyuk, G., Yigit, A.A., Aydogan, I. (2018): Co-Culture of Rat Luteal Cells with Islet Cells Enhances Islet Viability and Revascularization. In Vitro Cellular and Developmental Biology - Animal 54: 640–647.

Lucas-Clerc, C., Massart, C., Campion, J.P., Launois, B., Nicol, M. (1993): Long-Term Culture of Human Pancreatic Islets in an Extracellular Matrix: Morphological and Metabolic Effects. Molecular and Cell Endocrinology 94: 9–20.

Mccord, J.M. (1993): Human Disease, Free Radicals, and the Oxidant/Antioxidant Balance. Clinical Biochemistry 26: 351–357.

Halliwell, B. (2003): Oxidative Stress in Cell Culture: An Under-Appreciated Problem? FEBS Letters 540: 3–6.

Boyd, V., Cholewa, O.M., Papas, K.K. (2008): Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell Permeable Nucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Current Trends in Biotechnological Pharmacy 2: 66–84.

Dagli Gul, A.S., Fadillioglu, E., Karabulut, I., Yesilyurt, A., Delibasi, T. (2013): The Effects of Oral Carvacrol Treatment Against H2O2 Induced Injury on Isolated Pancreas Islet Cells of Rats. Islets 5: 149-155.

Togar, B., Turkez, H., Tatar, A., Hacimuftuoglu, A., Geyikoglu, F. (2015): Cytotoxicity and Genotoxicity of Zingiberene on Different Neuron Cell Lines In Vitro. Cytotechnology 67: 939–946.

Erel, O. (2005): A New Automated Colorimetric Method for Measuring Total Oxidant Status. Clinical Biochemistry 38: 1103–1011.

White, S.A, James, R.F.L., Swift, S.M, Kimber, R.M, Nicholson, M.L. (2001): Human Islet Cell Transplantation - Future Prospects. Diabetic Medicine 18: 78–103.

Bjare, U. (1992): Serum-Free Cell Culture. Pharmacology and Therapeutics 53: 355–374.

Rush, B.T., Fraga, D.W., Kotb, M.Y., Sabek, O.M., Lo, A., Gaber, L.W., Halim, A.B., Gaber, A.O. (2004): Preservation of Human Pancreatic Islet In Vivo Function After 6-Month Culture in Serum-Free Media. Transplantation 77: 1147–1154.

Rastellini, C., Cicalese, L., Zeevi, A., Mattes, C., Stanko., R.T., Starzl, T.E., Rao, A.S. (1995): Long-Term Culture of Viable Human Pancreatic Islets in Pyruvate-Rich Medium. In: Transplantation Proceedings. Elsevier USA. p. 3383–3384.

Gaber, A.O., Fraga, D. (2004): Advances in Long-Term Islet Culture: The Memphis Experience. Cell Biochemistry and Biophysics 40: 49–54.

Clark, S.A., Chick, W.L. (1990): Islet Cell Culture in Defined Serum-Free Medium. Endocrinology 126:1895–903.

Avgoustiniatos, E.S., Scott, W.E., Suszynski, T.M., Schuurman, H.J., Nelson, R.A., Rozak, R.R., Mueller, K.R., Balamurugan, A.N., Ansite, J.D., Fraga, D.W. (2012): Supplements in Human Islet Culture: Human Serum Albumin is Inferior to Fetal Bovine Serum. Cell Transplantation 21: 2805–2814.

Lee, J.J., Kwon, J.H., Park, Y.K., Kwon, O., Yoon, T.W. (1997): The Effects of Various Hormones and Growth Factors On the Growth of Human Insulin-Producing Cell Line In Serum-Free Medium. Experimental and Molecular Medicine 29: 209.

Fraga, D.W., Sabek, O., Hathaway, D.K., Gaber, A.O. (1998): A Comparison of Media Supplement Methods for the Extended Culture of Human Islet Tissue. Transplantation. 65: 1060–1066.

Gaber, A.O., Fraga, D.W., Callicutt, C.S., Gerling, I.C., Sabek, O.M., Kotb, M.Y. (2001): Improved in Vivo Pancreatic Islet Function After Prolonged In Vitro Islet Culture. Transplantation 72: 1730–1736.

Ling, Z., Hannaert, J.C., Pipeleers, D (1994): Effect of Nutrients, Hormones and Serum On Survival of Rat Islet Beta Cells in Culture. Diabetologia 37: 15–21.

Behboo, R. (1994): Improved Long-Term Culture of Functional Human Islets in Serum-Free Medium. Transplantation Proceedings 26: 3301.

Bertera, S., Balamurugan, A.N., Bottino, R., He, J., Trucco, M. (2012): Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin. Journal of Transplantation 2012: 1–9.

Ahangarpour, A., Heidari, H., Mard, S.A., Hashemitabar, M., Khodadadi, A. (2014): Progesterone and cilostazol protect mice pancreatic islets from oxidative stress induced by hydrogen peroxide. Iranian Journal of Pharmacological Resesearch 13: 937–944.

Carter, J., Karmiol, S., Nagy, M., McElreath, N., Calloway, C., Motley, A., Neill, A., Jang, H.J., Posselt, A., Stock, P. (2005): Pretransplant Islet Culture: A Comparison of Four Serum-Free Media Using a Murine Model of Islet Transplantation. Transplantation Proceedings 37: 3446–3449.

Murdoch, T.B., McGhee-Wilson, D., Shapiro, A.M.J., Lakey, J.R.T. (2004): Methods of Human Islet Culture for Transplantation. Cell Transplantation 13: 605–618.

Jackson, J.L., Chamberlin, J., Kroenke, K. (2001): Predictors of Patient Satisfaction. Social Science and Medicine 52: 609–620.

Raposo do Amaral, A.S., Pawlick, R.L., Rodrigues, E., Costal, F., Pepper, A., Ferreira Galvão, F.H., Correa-Giannella, M.L., Shapiro, A.M.J., Nadal, A. (2013): Glutathione Ethyl Ester Supplementation during Pancreatic Islet Isolation Improves Viability and Transplant Outcomes in a Murine Marginal Islet Mass Model. PLoS ONE 8: e55288.

Faure, P., Troncy, L., Lecomte, M., Wiernsperger, N., Lagarde, M., Ruggiero, D., Halimi, S. (2005): Albumin Antioxidant Capacity Is Modified by Methylglyoxal. Diabetes and Metabolism Journal 31: 169–177.

Corbin, K.L., West, H.L., Brodsky, S., Whitticar, N.B., Koch, W.J., Nunemaker, C.S. (2021): A Practical Guide to Rodent Islet Isolation and Assessment Revisited. Biological Procedures Online 23: 7.

Wang, Y., Danielson, K.K., Ropski, A., Harvat, T., Barbaro, B., Paushter, D., Qi, M., Oberholzer, J. (2013): Systematic Analysis of Donor and Isolation Factor’s Impact on Human Islet Yield and Size Distribution. Cell Transplantation 22: 2323–2333.

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Published

2022-05-29

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Boyuk, G., & Yigit, A. A. (2022). EFFECTS OF MEDIUM SUPPLEMENTS ON VIABILITY AND FUNCTIONALITY IN LONG-TERM CULTURED ISLET CELLS. Journal of Applied Biological Sciences, 16(2), 353–365. Retrieved from https://jabsonline.org/index.php/jabs/article/view/1038

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