MONOFLORAL BRASSICA NIGRA POLLEN IMPROVES OXIDATIVE STRESS AND METABOLIC PARAMETERS IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
Abstract views: 197 / PDF downloads: 346Keywords:
Diabetes mellitus, oxidative stress, black mustard, bee pollen, micronucleusAbstract
Diabetes mellitus is accompanied by metabolic and oxidative stress-related complications and supportive treatment with natural products might prevent or delay the progression of these complications. The present study hypothesized that Brassica nigra pollen (BNP), shown to have metabolic and antioxidative properties, can reduce oxidative stress and genotoxicity and improve metabolic parameters in diabetes mellitus. Diabetes was induced by intraperitoneal injection of STZ (65 mg/kg). The rats were divided into four groups; control rats; control group rats given BNP; diabetic rats; diabetic group rats given BNP. Rats were given with BNP for thirty days (350 mg/kg/day). Serum insulin, blood glucose, triglyceride, total cholesterol, high-density lipoprotein-cholesterol, alanine aminotransferase, aspartate aminotransferase levels were evaluated using an auto-analyzer. Plasma and tissue malondialdehyde (MDA) levels were measured with spectrophotometric methods. Serum paraoxonase (PON), arylesterase (ARE), superoxide dismutase, glutathione peroxidase activities were determined using commercial kits. Genotoxicity was determined by the bone marrow micronucleus (MN) method. BNP recovered the increased plasma and heart, muscle, liver, and kidney tissue MDA and serum glucose and total cholesterol levels. BNP increased serum PON, ARE activities and showed antigenotoxic activity by decreasing MN frequency in the BNP-treated diabetic rats. BNP has antihyperglycemic, antihyperlipidemic, antioxidant, and antigenotoxic properties and can be a promising supportive therapeutic agent in diabetes mellitus for improving treatment outcomes and reducing treatment-related and/or diabetes-related complications.
References
Giacco, F., Brownlee, M. (2010): Oxidative stress and diabetic complications. Circulation Research 107:1058–1070.
Volpe, C.M.O., Villar-Delfino, P.H., Dos Anjos, P.M.F., Nogueira-Machado. J.A. (2018): Cellular death, reactive oxygen species (ROS), and diabetic complications. Cell Death & Disease 9:119.
Forman, H.J., Zhang, H. (2021): Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nature Reviews Drug Discovery 30:1–21.
Chung, S.S., Ho, E.C., Lam, K.S., Chung, S.K. (2003): Contribution of polyol pathway to diabetes-induced oxidative stress. Journal of the American Society of Nephrology 14:S233–236.
Singh, R., Devi, S., Gollen, R. (2015): Role of free radical in atherosclerosis, diabetes, and dyslipidemia: larger-than-life. Diabetes/Metabolism Research and Reviews 31:113–126.
Dave, H.D., Preuss C.V. (2021): Human Insulin. In: StatPearls Publishing; Treasure Island (FL).
Stein, S.A., Lamos, E.M., Davis, S.N. (2013): A review of the efficacy and safety of the oral antidiabetic drug. Expert Opinion on Drug Safety 12:153–175.
Denisow. B., Denisow-Pietrzyk, M. (2016): Biological and therapeutic properties of bee pollen: A review. Journal of the Science of Food and Agriculture 96:4303–4309.
Linskens, H.F., Jorde, W. Pollen as food and medicine—A review. (1997): Economic Botany 51:78–86.
Komosińska-Vassev, K., Olczyk, P., Kázmierczak, J., Mencner, L., Olczyk, K. (2015): Bee pollen: Chemical composition and therapeutic application. Evidence-Based Complementary and Alternative Medicine. Med ID 297425, 1-6. http://dx.doi.org/10.1155/2015/297425.
Almaraz-Abarca, N., Campos, M.G., Ávila-Reyes, J.A., Naranjo-Jiménez, N., Corral, J.H., González-Valdez, L.S. (2007): Antioxidant activity of polyphenolic extract of monofloral honeybee-collected pollen from mesquite (Prosopis juliflora, Leguminosae). Journal of Food Composition and Analysis 20:119–124.
Anand, P., Murali, Y.K., Tandon, V., Chandra, R., Murthy, P.S. (2007): Preliminary studies on the antihyperglycemic effect of aqueous extract of Brassica nigra (L.) Koch in streptozotocin-induced diabetic rats. Indian Journal of Experimental Biology 45:696-701.
Anand, P., Murali, Y.K., Tandon, V., Murthy, P.S., Chandra, R. (2009): Insulinotropic Effect of Aqueous Extract of Brassica nigra Improves Glucose Homeostasis in Streptozotocin-Induced Diabetic Rats. Experimental and Clinical Endocrinology and Diabetes Latest 117:251-256.
Singhal, P., Singla, N., Sakhare, D., Sharma, A.K. (2017): A Comparative Evaluation of In-Vitro Antioxidant Activity of Some Commonly Used Spices of Northern India. The Natural Products Journal 7: 1-6.
Biljana, S.Đ., Dragan, Z.T., Zoran, B.T., Ivica, G.Đ., Ljiljana, P.S., Mitrović, P.M, Veljković, V.B. (2020): The effect of the triethanolamine: glycerol deep eutectic solvent on the yield, fatty acid composition, antioxidant activity and physicochemical properties of black mustard (Brassica nigra L.) seed oil. Journal of Food Measurement and Characterization 14:2570–2577.
Spulber, R., Doğaroğlu, M., Babeanu, N., Popa, O. (2018): Physicochemical characteristics of fresh bee pollen from different botanical origins. Romanian Biotechnological Letters 23:13357-13365.
Ketkar, S.S., Rathore, A.S., Lohidasan, S., Rao, L., Paradkar, A.R., Mahadik, K.R. (2014): Investigation of the nutraceutical potential of monofloral Indian mustard bee pollen. Journal of Complementary and Integrative Medicine 12:379-389.
Kalaycıoğlu, Z., Kaygusuz, H., Döker, S., Kolaylı, S., Erim, F.B. (2017): Characterization of Turkish honeybee pollens by principal component analysis based on their individual organic acids, sugars, minerals, and antioxidant activities. LWT– Food Science and Technology 84:402-408.
Ohkawa, H., Ohishi, N., Yagi, K. (1979): Assay for lipid peroxides in animal tissues by the thiobarbituric acid reaction. Analytical Biochemistry 95: 351–358.
Young, I.S., Trimble, E.R. (1991): Measurement of malondialdehyde in plasma by high-performance liquid chromatography with fluorimetric detection. Annals of Clinical Biochemistry 28: 504–508.
Waters, M.D., Brady, A.L., Stack, H.F., Brockman, H.E. (1990): Antimutagenicity profiles for some model compounds. Mutation Research 238:57–85.
Taş, S., Sarandöl, E., Dirican, M. (2014): Vitamin B6 supplementation improves oxidative stress and enhances serum paraoxonase/arylesterase activities in streptozotocin-induced diabetic rats. The Scientific World Journal 351598. DOI: 10.1155/2014/351598.
Sarandol, E., Tas, S., Serdar, Z., Dirican, M. (2020): Effects of thiamine treatment on oxidative stress in experimental diabetes. Bratislava Medical Journal 121:235–241.
Bassalat, N., Taş, S., Jaradat, N. (2020): Teucrium leucocladum: An Effective Tool for the Treatment of Hyperglycemia, Hyperlipidemia, and Oxidative Stress in Streptozotocin-Induced Diabetic Rats. Evidence-based Complementary and Alternative Medicine 8. https://doi.org/10.1155/2020/3272103.
Amal, D., Drira, M., Sana, B., Najla, H., Kais, M., Adel, K., Néji, GH. (2015): Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of Date Palm Pollen (DPP) from two Tunisian cultivars. Arabian Journal of Chemistry http://doi.org/10.1016/j.arabjc.
Dragan, S., Andrica M.F., Maria-Corina, S., Timar, R. (2015): Polyphenols-rich natural products for the treatment of diabetes. Current Medicinal Chemistry 21:14-22.
Hamden, K., Keskes, H., Belhaj, S., Mnafgui, K., Feki, A., Allouche, N. (2011): Inhibitory potential of omega-3 fatty and fenugreek essential oil on key enzymes of carbohydrate-digestion and hypertension in diabetes rats. Lipids in Health and Disease 10:226. DOI: 10.1186/1476-511X-10-226.
Hogan, S., Zhang, L., Li, J., Sun, S., Canning, C., Zhou, K. (2010): Antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase. Nutrition & Metabolism 7:71. DOI: 10.1186/1743-7075-7-71.
Pereira, D.F., Cazarolli, L.H., Lavado, C., Mengatto, V., Santos, Figueiredo, M.R.B., Guedes, A., Pizzolatti, M.G., Silva, F.R.M.B. (2011): Effects of flavonoids on α-glucosidase activity: potential targets for glucose homeostasis. Nutrition 27:1161–1167.
Sireesha, Y., Kasetti, R.B., Nabi, S.A., Swapna, S., Apparao, C. (2011): Antihyperglycemic and hypolipidemic activities of Setaria italica seeds in STZ diabetic rats. Pathophysiology18:159–164.
Gaweł, S., Wardas, M., Niedworok, E., Wardas, P. (2004): Malondialdehyde (MDA) as a lipid peroxidation marker. Wiadomosci lekarskie 57:453-455.
Karadal, F., Onmaz, N.E., Abay, S., Yildirim, Y., Al, S., Tatyuz, I., Akcay, Aytac. (2018): A Study of Antibacterial and Antioxidant Activities of Bee Products: Propolis, Pollen and Honey Samples. The Ethiopian Journal of Health Development 32:116-122.
Saral, Ö., Yıldız, O., Yazıcıoğlu, RA., Yuluğ, E., Canpolat, S., Öztürk, F., Kolaylı S. (2016): Apitherapy products enhance the recovery of CCL4-induced hepatic damages in rats. Turkish Journal Of Medical Sciences 46:194–202.
Ulusoy, E., Kolayli, S. (2013): Phenolic composition and antioxidant properties of Anzer bee pollen. Journal of Food Biochemistry 38:73–82.
Rajamurugan, R., Selvaganabathy, N., Kumaravel, S., Ramamurthy, C.H., Sujatha, V., Thirunavukkarasu, C. (2012): Polyphenol contents and antioxidant activity of Brassica nigra (L.) Koch. leaf extract. Natural Product Research 26:2208-2210.
Laaroussi, H., Bakour, M., Ousaaid, D., Aboulghazi, A., Ferreira-Santos P., Genisheva, Z., Teixeira, J.A., Lyoussia, B. (2020): Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced type 2 diabetes in rats. Food Research International 138:109802 https://doi.org/10.1016/j.foodres.2020.109802.
Lewandowski, L., Kepinska, M., Milnerowicz, H. (2019): The copper‐zinc superoxide dismutase activity in selected diseases. European Journal of Clinical Investigation 49:e13036 DOI: 10.1111/eci.13036.
Huanga, J.Q., Zhouc, J.C., Wua, Y.Y., Rena, F.Z., Le, X.G. (2018): Role of glutathione peroxidase 1 in glucose and lipid metabolism-related diseases. Free Radical Biology and Medicine 127:108–115. doi:10.1016/j.freeradbiomed.2018.05.077
Ighodaro, O.M., Akinloye, O.A. (2018): First line defense antioxidants-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defense grid. Alexandria Journal of Medicine 54:287-293.
Meneses, M.J., Silvestre, R., Sousa-Lima, I., Macedo, M.P. (2019): Paraoxonase-1 as a Regulator of Glucose and Lipid Homeostasis: Impact on the Onset and Progression of Metabolic Disorders. International Journal of Molecular Sciences 20:4049. DOI: 10.3390/ijms20164049.
Aviram, M., Fuhrman, B. (1998): LDL oxidation by arterial wall macrophages depends on the oxidative status in the lipoprotein and the cells: role of prooxidants vs. antioxidants. Molecular and Cellular Biochemistry 188: 149–159.
Tsuzura, S., Ikeda, Y., Suehiro, T., Ota, K., Osaki, F., Arii, K., Kumon, Y., Hashimoto, K. (2004): Correlation of plasma oxidized low-density lipoprotein levels to vascular complications and human serum paraoxonase in patients with type 2 diabetes. Metabolism: Clinical and Experimental 53: 297–302.
Karakaya, P., Ozdemir, B., Mert, M., Okuturlar, Y. (2018): Relation of Paraoxonase 1 Activity with Biochemical Variables, Brachial Artery Intima-Media Thickness in Patients with Diabetes with or without Obesity. Obesity Facts 11:56–66.
Vikram, A., Tripathi, D.N., Ramarao, P., Jena, G.B. (2007): Evaluation of streptozotocin genotoxicity in rats from different ages using the micronucleus assay. Regulatory Toxicology and Pharmacology 49:238–244.
Kita-Tomihara, T., Sato, S., Yamasaki, S., Ueno, Y., Kimura, G., Ketema, R.M., Kawahara, T., Kurasaki, M., Sait, T. (2019): Polyphenol-enriched azuki bean (Vina angularis) extract reduces the oxidative stress and prevents DNA oxidation in the hearts of streptozotocin-induced early diabetic rats. International Journal of Food Science 70: 845-855.
Bolzán, A.D., Bianchi, M.S. (2002): Genotoxicity of streptozotocin. Mutation Research 512:121–134.
Stopper, H., Müller, S.O. (1997): Micronuclei as a biological endpoint for genotoxicity: A minireview. Toxicology in Vitro 11:661–667.
Lee, S.C., Chan, J.C.N. (2015): Evidence for DNA Damage as a Biological Link Between Diabetes and Cancer. Chinese Medical Journal 128:1543-1548. DOI: 10.4103/0366-6999.157693.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Journal of Applied Biological Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
JABS is licensed under a 