DETERMINATION OF ANTIBIOTIC RESISTANCE PROFILES IN ACINETOBACTER SPECIES ISOLATED FROM TANK MILK

Abstract

The aim of this study was to determine the occurrence of Acinetobacter spp. in tank milk samples (TMS) in Hatay province and to analyze their antimicrobial susceptibility profile. In this study, a total of 60 TMS were collected between April and December 2016. Acinetobacter spp. was identified by using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry (MS). The identified Acinetobacter isolates were tested to determine the antimicrobial resistance profiles towards 23 antibiotics using the disc diffusion method. A total of 13 Acinetobacter isolates were obtained from TMS (21.6%).  Species distribution were as follow; Acinetobacter baumannii (n=9), Acinetobacter ursingii (n=3) and Acinetobacter iwoffii (n=1). Resistance to aztreonam, chloramphenicol, amoxicillin and clavulanate, cefoxitin, cefpodoxime gentamicin, cefuroxime, florphenicol was 53.8%, 15.3% 15.3%, 15.3%, 15.3%, 7.7%, 7.7%, and 7.7%, respectively. However, none of the isolates was resistant to nalidixic acid, ciprofloxacin, enrofloxacin, levofloxacin, norfloxacin, penicillin, tetracycline, sulfamethoxazole/trimethoprim, ampicillin, imipenem, colistin, cephalothin, and ceftazidime. As a result, the TMS were found to be contaminated with different species of Acinetobacter. Another important finding of this study is the antimicrobial resistance characteristics of these strains, which might pose a public health problem. Therefore, it may be said that TMS would be better to be monitored for this potentially pathogenic bacterium.