DETERMINATION OF ANTIBIOTIC RESISTANCE PROFILES IN ACINETOBACTER SPECIES ISOLATED FROM TANK MILK
DOI:
https://doi.org/10.71336/jabs.1109Keywords:
Acinetobacter, tank milk, antibiotic resistanceAbstract
The aim of this study was to determine the occurrence of Acinetobacter spp. in tank milk samples (TMS) in Hatay province and to analyze their antimicrobial susceptibility profile. In this study, a total of 60 TMS were collected between April and December 2016. Acinetobacter spp. was identified by using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry (MS). The identified Acinetobacter isolates were tested to determine the antimicrobial resistance profiles towards 23 antibiotics using the disc diffusion method. A total of 13 Acinetobacter isolates were obtained from TMS (21.6%). Species distribution were as follow; Acinetobacter baumannii (n=9), Acinetobacter ursingii (n=3) and Acinetobacter iwoffii (n=1). Resistance to aztreonam, chloramphenicol, amoxicillin and clavulanate, cefoxitin, cefpodoxime gentamicin, cefuroxime, florphenicol was 53.8%, 15.3% 15.3%, 15.3%, 15.3%, 7.7%, 7.7%, and 7.7%, respectively. However, none of the isolates was resistant to nalidixic acid, ciprofloxacin, enrofloxacin, levofloxacin, norfloxacin, penicillin, tetracycline, sulfamethoxazole/trimethoprim, ampicillin, imipenem, colistin, cephalothin, and ceftazidime. As a result, the TMS were found to be contaminated with different species of Acinetobacter. Another important finding of this study is the antimicrobial resistance characteristics of these strains, which might pose a public health problem. Therefore, it may be said that TMS would be better to be monitored for this potentially pathogenic bacterium.
References
Tamang, M. D., Gurung, M., Nam, H. M., Kim, S. R., Jang, G. C., Jung, S. C. (2014): Short communication: genetic characterization of antimicrobial resistance in Acinetobacter isolates recovered from bulk tank milk. J. Dairy Sci. 97, 704–709. DOI: 10.3168/jds.2013-7403. DOI: https://doi.org/10.3168/jds.2013-7403
Nahla A. Abou El-Roos; Ebtesam M. Mazıd; Eman M. Zakary and Kayrı F. Abou El Yazıd. (2013): Molecular Characterızatıon of Pseudomonas Aerugınosa Isolated from Mılk. J. Assiut Vet. Med59, 14-22. DOI: 10.21608/avmj.2013.171897. DOI: https://doi.org/10.21608/avmj.2013.171897
Berhe, G., Wasihun, A.G., Kassaye, E., Gebreselasie, K. (2020): Milk-borne bacterial health hazards in milk produced for commercial purpose in Tigray, northern Ethiopia. BMC Public Health. 20:894. https://doi.org/10.1186/s12889-020-09016-6. DOI: https://doi.org/10.1186/s12889-020-09016-6
Saad, N.M., Amın W.F., Mostafa, S.M. (2018): Detectıon of Acınetobacter Specıes in Mılk and Some Daıry Products. Assiut Vet. Med. J. Vol. 64 No. 156 2018, 34-40. DOI: 10.21608/AVMJ.2018.168683. DOI: https://doi.org/10.21608/avmj.2018.168683
Claeys W, Cardoen S, Daube G, De Block J, Dewettinck K, Dierick K. (2013): Raw or Heated Cow Milk Consumption: Review of Risks and Benefits. Food Control. 31:251e262. https://doi.org/10.1016/j.foodcont.2012.09.035. DOI: https://doi.org/10.1016/j.foodcont.2012.09.035
Gurung M., Nam H. M., Tamang M. D., Chae M. H., Jang G. C., Jung S. C., and Lim S. K. (2013): Prevalence and antimicrobial susceptibility of Acinetobacter from raw bulk tank milk in Korea J. Dairy Sci. 96:1997–2002. DOI: 10.3168/jds.2012-5965. DOI: https://doi.org/10.3168/jds.2012-5965
Manchanda V., Sanchaita S., Singh NP. (2010): Resistant Acinetobacter significance and control. J Global Infect Dis. 2:291-304. DOI: 10.4103/0974-777X.68538. DOI: https://doi.org/10.4103/0974-777X.68538
Doughari, H.J.; Ndakidemi, P.A.; Human, I.S. and Benade, S. (2011): The ecology, biology, and pathogenesis of Acinetobacter spp. an overview. Microbes and Environments/JSME, 26(2): 101-112. DOI: 10.1264/jsme2.me10179. DOI: https://doi.org/10.1264/jsme2.ME10179
Asif M., Alvi I.A., Rehman S.U. (2018): Insight into Acinetobacter baumannii: pathogenesis global resistance, mechanisms of resistance, treatment options, and alternative modalities. Infect Drug Resist. 11:1249-60. DOI: 10.2147/IDR.S166750. DOI: https://doi.org/10.2147/IDR.S166750
Moreira Silva G, Morais L, Marques L, Senra V. (2012): Acinetobacter community-acquired pneumonia in a healthy child. Rev Port Pneumol. 18:96-8. DOI: 10.1016/j.rppnen.2011.12.005. DOI: https://doi.org/10.1016/j.rppnen.2011.12.005
Tacconelli E and Magrini N. (2018): Global Priority List of Antibiotic-Resistant Bacteria to Guide Research, Discovery, and Development of New Antibiotics. World Health Organization (WHO). DOI: 10.1016/S1473-3099(17)30753-3. DOI: https://doi.org/10.1016/S1473-3099(17)30753-3
Centers for Disease Control (CDC). (2018): Available from: www.cdc.gov/drugresistance/biggest_ treat.html.
Amorim AM, Nascimento JD. (2017): Acinetobacter: an underrated foodborne pathogen? J Infect Dev Ctries. 11:111-4. DOI: 10.3855/jidc.8418. DOI: https://doi.org/10.3855/jidc.8418
Ramos GLPA, Nascimento JS. (2019): Characterization of Acinetobacter spp. from raw goat milk. Cienc Rural. 49: e20190404. https://doi.org/10.1590/0103-8478cr20190404. DOI: https://doi.org/10.1590/0103-8478cr20190404
Jain, R., and L. H. Danziger. (2004): Multidrug-resistant Acinetobacter infections: An emerging challenge to clinicians. Ann. Pharmacother. 38:1449–1459. DOI: 10.1345/aph.1D592. DOI: https://doi.org/10.1345/aph.1D592
Gallego, L., and K. J. Towner. (2001): Carriage of class 1 integrons and antibiotic resistance in clinical isolates of Acinetobacter baumannii from northern Spain. J. Med. Microbiol. 50:71–77. DOI:10.1099/0022-1317-50-1-71. DOI: https://doi.org/10.1099/0022-1317-50-1-71
Jayarao, B. M., and L. Wang. (1999): A study on the prevalence of gram- negative bacteria in bulk-tank milk. J. Dairy Sci. 82:2620–2624. DOI: 10.3168/jds. S0022-0302(99)75518-9. DOI: https://doi.org/10.3168/jds.S0022-0302(99)75518-9
Ndegwa E. N., Mulei C. M., and Munyua S. J. (2001): Prevalence of microorganisms associated with udder infections in dairy goats on small-scale farms in Kenya. J. S. Afr. Vet. Assoc. 72:97–98. DOI: 10.4102/jsava. v72i2.627. DOI: https://doi.org/10.4102/jsava.v72i2.627
Nam H.M., Lim S.K., Kang H.M., Kim J.M., Moon J.S., Jang K.C., Kim J.M., Joo Y.S., Jung S.C. (2009): Prevalence and antimicrobial susceptibility of gram-negative bacteria isolated from bovine mastitis between 2003 and 2008 in Korea. J. Dairy Sci. 92:2020–2026. DOI: 10.3168/jds.2008-1739. DOI: https://doi.org/10.3168/jds.2008-1739
Straley, B. A., Donaldson, S. C., Hedge, N. V., Sawant, A. A., Srinivasan, V., Oliver, S. P. (2006): Public health significance of antimicrobial-resistant gram- negative bacteria in raw bulk tank milk. Foodborne Pathog. Dis. 3, 222–233. DOI: 10.1089/fpd.2006.3.222. DOI: https://doi.org/10.1089/fpd.2006.3.222
Kim C.U., Lee C. H., Kim J.H., Park Y.K., Shin S., Park K.T., Park H.Y. (2017): Prevalence and antimicrobial susceptibility of Acinetobacter baumannii in raw milk in Korea. Cheong Ung Kim et al. Appli Micro, 3:3 (Suppl) DOI: 10.4172/2471-9315-C1-009. DOI: https://doi.org/10.4172/2471-9315-C1-009
Cho G.S., Li B., Rostalsky A., Fiedler G., Rösch N., Igbinosa E., Kabisch J., Bockelmann W., Hammer P., Huys G. (2018): Diversity and Antibiotic Susceptibility of Acinetobacter Strains from Milk Powder Produced in Germany. Front. Microbiol. 9:536. DOI: 10.3389/fmicb.2018.00536. DOI: https://doi.org/10.3389/fmicb.2018.00536
Temel A, Eraç B. Küresel bir tehdit: Acinetobacter baumannii, antimikrobiyal dirençte güncel durum ve alternatif tedavi yaklaşımları. Turk Hij Den Biyol Derg, 2020; 77(3): 367-378. DOI: 10.5505/TurkHijyen.2019.04764. DOI: https://doi.org/10.5505/TurkHijyen.2019.04764
Savcı Ü., Özveren G., Yenişehirli G., Bulut Y., Özdaş S., (2020): Klinik örneklerden izole edilen Acinetobacter baumannii suşlarının in- vitro duyarlılık durumları. Turk Journal of Clin and Lab. 2015; 6:24-9. https://doi.org/10.18663/tjcl.42209. DOI: https://doi.org/10.18663/tjcl.42209
Kanaan Ghaffoori M.H., et al. (2020): Drug resistance and virulence traits of Acinetobacter baumannii from Turkey and chicken raw meat. Comparative Immunology, Microbiology, and Infectious Diseases 70, 101451. DOI: 10.1016/j.cimid.2020.101451. DOI: https://doi.org/10.1016/j.cimid.2020.101451
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Journal of Applied Biological Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
