EFFECT OF PYRACLOSTROBIN-BASED HERBICIDE ON DNA DAMAGE AND REPRODUCTIVE PERFORMANCE IN DROSOPHILA MELANOGASTERS

Authors

  • Fahriye Zemheri Navruz
  • Özge Çeliktaş Köstekçi
  • Sinan İnce

DOI:

https://doi.org/10.71336/jabs.1197

Keywords:

Drosophila melanogaster, DNA damage, larval toxicity, pyraclostrobin, reproductive performance

Abstract

The increasingly uncontrolled proliferation of pesticide use today affects the life of all creatures negatively. Pyraclostrobin (Pyra) is a strobilurin class fungicide, which has been widely used in recent years and is known to be toxic to aquatic species. In this study, the effects of Pyra at increased concentrations between 25-1000 µg/L on reproductive performance and genotoxicity of a model organism, Drosophila melanogaster, were investigated. It was found that Pyra decreased reproductive performance and pupa formation from larvae in D. melonagasters in parallel with the dose increase. In addition, it was found that Pyra concentrations (between 200-1000 µg/L) administered to male and female adults caused DNA damage in D. melonagasters, in parallel with their increased concentrations. As a result, it was concluded that Pyra at high concentrations may have a negative effect on D. melanogasters and therefore on the ecosystem of the living creatures.

References

Tang, F. H., Lenzen, M., McBratney, A., & Maggi, F. (2021): Risk of pesticide pollution at the global scale. Nature Geoscience 14(4): 206-210. https://doi.org/10.1038/s41561-021-00712-5. DOI: https://doi.org/10.1038/s41561-021-00712-5

Grzywacz, J. G., Gonzales-Backen, M., Liebman, A., Marín, A. J., Trejo, M., Gudino, C. O., ... & Tovar-Aguilar, J. A. (2019): Attending to pesticide exposure and heat illness among farmworkers: Results from an attention placebo-controlled evaluation design. Journal of Occupational and Environmental Medicine 61(9): 735-742. https://doi.org/10.1097/JOM.0000000000001650. DOI: https://doi.org/10.1097/JOM.0000000000001650

Cui, F., Chai, T., Liu, X., & Wang, C. (2017): Toxicity of three strobilurins (kresoxim‐methyl, pyraclostrobin, and trifloxystrobin) on Daphnia magna. Environmental toxicology and chemistry 36(1): 182-189. https://doi.org/10.1002/etc.3520. DOI: https://doi.org/10.1002/etc.3520

Hou, K., Shi, B., Liu, Y., Lu, C., Li, D., Du, Z., ... & Zhu, L. (2022): Toxicity evaluation of Pyraclostrobin exposure in farmland soils and co-exposure with nZnO to Eisenia fetida. Journal of Hazardous Materials 433: 128794. https://doi.org/10.1016/j.jhazmat.2022.128794. DOI: https://doi.org/10.1016/j.jhazmat.2022.128794

Bartholomaeus, A., (2003): Pyraclostrobin, Office of Chemical Safety, Therapeutic Goods Administration, Canberra, Australia 275–319 JMPR 2003.

Fan, L., Huang, Y., Huang, T., Zhao, K., Zhang, Y. N., Li, C., & Zhao, Y. H. (2020): Photolysis and photo-induced toxicity of pyraclostrobin to Vibrio fischeri: Pathway and toxic mechanism. Aquatic Toxicology 220: 105417. https://doi.org/10.1016/j.aquatox.2020.105417. DOI: https://doi.org/10.1016/j.aquatox.2020.105417

Reilly, T. J., Smalling, K. L., Orlando, J. L., & Kuivila, K. M. (2012): Occurrence of boscalid and other selected fungicides in surface water and groundwater in three targeted use areas in the United States. Chemosphere 89(3): 228-234. https://doi.org/10.1016/j.chemosphere.2012.04.023. DOI: https://doi.org/10.1016/j.chemosphere.2012.04.023

Mimbs IV, W. H., Cusaac, J. P. W., Smith, L. M., McMurry, S. T., & Belden, J. B. (2016): Occurrence of current-use fungicides and bifenthrin in Rainwater Basin wetlands. Chemosphere 159: 275-281. https://doi.org/10.1016/j.chemosphere.2016.06.012. DOI: https://doi.org/10.1016/j.chemosphere.2016.06.012

Guo, X., Wu, W., Song, N., Li, J., Kong, D., Kong, X., ... & Shan, Z. (2017): Residue dynamics and risk assessment of pyraclostrobin in rice, plants, hulls, field soil, and paddy water. Human and Ecological Risk Assessment: An International Journal 23(1): 67-81. https://doi.org/10.1080/10807039.2016.1222579. DOI: https://doi.org/10.1080/10807039.2016.1222579

Li, H., Zhao, F., Cao, F., Teng, M., Yang, Y., & Qiu, L. (2019): Mitochondrial dysfunction-based cardiotoxicity and neurotoxicity induced by pyraclostrobin in zebrafish larvae. Environmental Pollution 251: 203-211. https://doi.org/10.1016/j.envpol.2019.04.122. DOI: https://doi.org/10.1016/j.envpol.2019.04.122

Ma, J., Cheng, C., Du, Z., Li, B., Wang, J., Wang, J., ... & Zhu, L. (2019): Toxicological effects of pyraclostrobin on the antioxidant defense system and DNA damage in earthworms (Eisenia fetida). Ecological Indicators 101: 111-116. https://doi.org/10.1016/j.ecolind.2019.01.015. DOI: https://doi.org/10.1016/j.ecolind.2019.01.015

Sun Y, Yolitz J, Wang C, Spangler E, Zhan M, & Zou S. (2013): Aging studies in Drosophila melanogaster. In Biological Aging (pp. 77-93). Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-556-9_7. DOI: https://doi.org/10.1007/978-1-62703-556-9_7

Anupama KP, Shilpa O, Anet A, Siddanna TK, Gurushankara HP. (2019): Convolvulus pluricaulis (Shankhapushpi) ameliorates human microtubule-associated protein tau (hMAPτ) induced neurotoxicity in Alzheimer's disease Drosophila model. Journal of Chemical Neuroanatomy 95: 115-122. https://doi.org/10.1016/j.jchemneu.2017.10.002. DOI: https://doi.org/10.1016/j.jchemneu.2017.10.002

Quesada‐Calderón S, Bacigalupe LD, Toro‐Vélez AF, Madera‐Parra CA, Peña‐Varón MR, & Cárdenas‐Henao H. (2017): The multigenerational effects of water contamination and endocrine disrupting chemicals on the fitness of Drosophila melanogaster. Ecology and Evolution 7(16): 6519-6526. doi: 10.1002/ece3.3172. https://doi.org/10.1002/ece3.3172. DOI: https://doi.org/10.1002/ece3.3172

Zhang, C., Wang, J., Zhang, S., Zhu, L., Du, Z., Wang, J, (2017): Acute and subchronic toxicity of pyraclostrobin in zebrafish (Danio rerio). Chemosphere 188: 510-516. https://doi.org/10.1016/j.chemosphere.2017.09.025. DOI: https://doi.org/10.1016/j.chemosphere.2017.09.025

Uysal H, Semerdoken S. The assesment of longevity effects and larval toxicity of synthetic food dyes on Oregon R wild type of Drosophila melanogaster. Kafkas Uni. J. Grad. School Nat. Applied Sci 4(1): 71-87.

Abolaji, A. O., Kamdem, J. P., Lugokenski, T. H., Nascimento, T. K., Waczuk, E. P., Farombi, E. O., ... & Rocha, J. B. T. (2014): Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster. Free Radical Biology and Medicine 71:99-108. https://doi.org/10.1016/j.freeradbiomed.2014.03.014. DOI: https://doi.org/10.1016/j.freeradbiomed.2014.03.014

Pompa, P. P., Vecchio, G., Galeone, A., Brunetti, V., Sabella, S., Maiorano, G., Falqui, A., Bertoni, G., Cingolani, R. (2011): In vivo toxicity assessment of gold nanoparticles in Drosophila melanogaster. Nano Research 4(4): 405-413. https://doi.org/10.1007/s12274-011-0095-z. DOI: https://doi.org/10.1007/s12274-011-0095-z

Alexander, E. M., Aguiyi, J. C., Mdekera, I. W., Ogwu, O. S., Imoleayo, O. O., Ugokwe, C. V., & Pam, D. (2019): The climbing performance, neuromuscular transmitter (ACHE) activity, reproductive performance and survival of Drosophila melanogaster fed diet with Mangifera indica cold aqueous leaf extract. Journal of Applied Life Sciences International 1-11. https://doi.org/10.9734/JALSI/2019/v22i230120. DOI: https://doi.org/10.9734/jalsi/2019/v22i230120

Dhawan, A., Bajpayee, M. M., Pandey, A. K., & Parmar, D. (2003): Protocol for the single cell gel electrophoresis/comet assay for rapid genotoxicity assessment. Sigma 1077(1): 1-10.

Olive PL, & Banáth JP. (2006): The comet assay: a method to measure DNA damage in individual cells. Nature Protocols | VOL.1 NO.1: 23-29. https://doi.org/10.1038/nprot.2006.5. DOI: https://doi.org/10.1038/nprot.2006.5

Kissoum, N., Bensafi-Gheraibia, H., Hamida, Z. C., & Soltani, N. (2020): Evaluation of the pesticide Oberon on a model organism Drosophila melanogaster via topical toxicity test on biochemical and reproductive parameters. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 228: 108666. https://doi.org/10.1016/j.cbpc.2019.108666. DOI: https://doi.org/10.1016/j.cbpc.2019.108666

Ahmed, M. A. I., Vogel, C. F. A., & Malafaia, G. (2022): Short exposure to nitenpyram pesticide induces effects on reproduction, development and metabolic gene expression profiles in Drosophila melanogaster (Diptera: Drosophilidae). Science of The Total Environment 804: 150254. https://doi.org/10.1016/j.scitotenv.2021.150254. DOI: https://doi.org/10.1016/j.scitotenv.2021.150254

Li, H., Yang, S., Li, T., Li, X., Huang, X., Gao, Y., ... & Mu, W. (2020): Determination of pyraclostrobin dynamic residual distribution in tilapia tissues by UPLC-MS/MS under acute toxicity conditions. Ecotoxicology and Environmental Safety 206: 111182. https://doi.org/10.1016/j.ecoenv.2020.111182. DOI: https://doi.org/10.1016/j.ecoenv.2020.111182

Oulhaci, C. M., Denis, B., Kilani‐Morakchi, S., Sandoz, J. C., Kaiser, L., Joly, D., & Aribi, N. (2018): Azadirachtin effects on mating success, gametic abnormalities and progeny survival in Drosophila melanogaster (Diptera). Pest Management Science 74(1): 174-180. https://doi.org/10.1002/ps.4678. DOI: https://doi.org/10.1002/ps.4678

Aribi, N., Oulhaci, M. C., Kilani-Morakchi, S., Sandoz, J. C., Kaiser, L., Denis, B., & Joly, D. (2017): Azadirachtin impact on mate choice, female sexual receptivity and male activity in Drosophila melanogaster (Diptera: Drosophilidae). Pesticide Biochemistry and Physiology 143: 95-101. https://doi.org/10.1016/j.pestbp.2017.09.002. DOI: https://doi.org/10.1016/j.pestbp.2017.09.002

Wang, L. X., Zhang, Y. C., Tao, S., Guo, D., Zhang, Y., Jia, Y. L., ... & Wu, S. F. (2020): Pymetrozine inhibits reproductive behavior of brown planthopper Nilaparvata lugens and fruit fly Drosophila melanogaster. Pesticide Biochemistry and Physiology 165: 104548. https://doi.org/10.1016/j.pestbp.2020.02.014. DOI: https://doi.org/10.1016/j.pestbp.2020.02.014

Li, X., Liu, J., & Wang, X. (2020): Exploring the multilevel hazards of thiamethoxam using Drosophila melanogaster. Journal of Hazardous Materials 384: 121419. https://doi.org/10.1016/j.jhazmat.2019.121419. DOI: https://doi.org/10.1016/j.jhazmat.2019.121419

Demir, E. (2012): In vivo genotoxicity assessment of diflubenzuron and spinosad in Drosophila melanogaster with the comet assay using haemocytes and the SMART assay. Fresenius Environmental Bulletin 21(12a): 3894-3900.

Sharma, A., Mishra, M., Shukla, A. K., Kumar, R., Abdin, M. Z., & Chowdhuri, D. K. (2012): Organochlorine pesticide, endosulfan induced cellular and organismal response in Drosophila melanogaster. Journal of Hazardous Materials 221: 275-287. https://doi.org/10.1016/j.jhazmat.2012.04.045. DOI: https://doi.org/10.1016/j.jhazmat.2012.04.045

Mukhopadhyay, I., Chowdhuri, D. K., Bajpayee, M., & Dhawan, A. (2004): Evaluation of in vivo genotoxicity of cypermethrin in Drosophila melanogaster using the alkaline Comet assay. Mutagenesis 19(2): 85-90. https://doi.org/10.1093/mutage/geh007. DOI: https://doi.org/10.1093/mutage/geh007

Rajak, P., Dutta, M., Khatun, S., Mandi, M., & Roy, S. (2017): Exploring hazards of acute exposure of Acephate in Drosophila melanogaster and search for l-ascorbic acid mediated defense in it. Journal of Hazardous Materials 321: 690-702. https://doi.org/10.1016/j.jhazmat.2016.09.067. DOI: https://doi.org/10.1016/j.jhazmat.2016.09.067

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Published

2023-05-31

How to Cite

Zemheri Navruz, F., Çeliktaş Köstekçi, Özge, & İnce, S. . (2023). EFFECT OF PYRACLOSTROBIN-BASED HERBICIDE ON DNA DAMAGE AND REPRODUCTIVE PERFORMANCE IN DROSOPHILA MELANOGASTERS. Journal of Applied Biological Sciences, 17(2), 266–275. https://doi.org/10.71336/jabs.1197

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