IN VITRO STUDY: ANTIVIRULENCE EFFECT OF CINNAMOMUM VERUM AGAINST STREPTOCOCCUS MUTANS - MAIN CAUSATIVE AGENT OF DENTAL CARIES
DOI:
https://doi.org/10.71336/jabs.1322Keywords:
Antibiotic resistance, biofilm, Cinnamomum verum, inhibitor, Streptococcus mutans, virulence factorsAbstract
Streptococcus mutans (S. mutans) predominantly resides in biofilms that develop on teeth, commonly referred to as dental plaque. The objective of the study is to explore the antimicrobial and antibiofilm efficacy of the Cinnamomum verum (C. verum) plant extract against an isolated strain of S. mutans. We assessed the antimicrobial and antibiofilm effectiveness of a natural plant extract derived from C. verum against S. mutans using a variety of in vitro tests, including the crystal violet biofilm inhibition assay and growth curve analyses, and then quantified extracellular polymeric substances (EPS) using ethanol extract of C. verum. At a concentration of 5 mg/mL, the ethanol extract obtained from C. verum showed inhibition of S. mutans. In the biofilm assay, statistical analysis indicated a 65.25% inhibition of biofilm formation at a subinhibitory dose of 1.25 mg/mL, without impacting the growth of S. mutans, and also at 1.25 mg/mL C. verum inhibited EPS production of S. mutans to 41.55 %. In summary, our investigations revealed that C. verum exhibits both antimicrobial and antibiofilm activities against S. mutans. The present study found that the ethanol extract derived from C. verum has antibacterial properties, and in vitro studies show a noteworthy decrease in biofilm formation. C. verum could serve as a potent remedy for diverse infections linked to dental cavities, highlighting its potential as a safe and effective alternative treatment option.
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