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Heat, local, measurement, temperature, wound model


Non-contact methods such as infrared thermography (IRT) stand out with their important advantages such as reducing the risk of contamination, providing real-time results, quantitatively determining a relative finding such as pain, being economical, easy, non-invasive, and safe. The study was aimed to evaluate the differences of intra-wound, wound edge, and non-wound local temperatures in untreated and treated wounds of a full-thickness excisional skin wound model by IRT in rats. Twelve Wistar albino rats (200–300 g, 8 weeks old, male) were used in the study. A full-thickness excisional skin wound (2.25 cm2) was created in the dorsal interscapular region of animals under general anesthesia. Animals were randomly divided into two groups (n=6), Group 1 (control) and Group 2 (dexpanthenol treatment), and the wound surface was treated topically once a day. Images were taken from the wound area of all animals with an infrared thermography camera 0, 1, 7, and 14 days after the wound creation. Minimum and maximum temperatures were measured with the Trotec IC-IR Report software for IRT in three different areas as intra-wound, wound margin, and non-wound. Local temperature changes were examined and analyzed statistically. In addition, the time/local temperature correlation was analyzed for the wound treatment. In the results, it was determined that the local temperature increase was less as the distance from the wound center and the wound treatment time progressed (p<0.05). Compared to Group 1, it was determined that there was less local temperature increase and less inflammation in Group 2 as the treatment progressed (p<0.05). In conclusion, this study determined the reference values for the use of IRT in wound healing studies in the full-thickness excisional skin wound model in rats. Further research is needed to standardize the methodology for using IRT in other wound models, such as infected wounds and diabetic wounds.


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How to Cite

Deveci, M. Z. Y., Işler, C. T., & Kırgız, Ömer. (2022). INVESTIGATION OF WOUND HEALING BY INFRARED THERMOGRAPHY IN A FULL-THICKNESS SKIN WOUND MODEL IN RATS. Journal of Applied Biological Sciences, 16(3), 493–502. Retrieved from