EVALUATION OF THE ANTIOXIDATIVE EFFECTS OF HIBISCUS SABDARIFFA L. EXTRACT ON SACCHAROMYCES CEREVISIAE WITH MOLECULAR BIOLOGICAL AND BIOCHEMICAL BIOMARKERS
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Hibiscus sabdariffa L., oxidative stress, Saccharomyces cerevisiae, SDS-PAGEAbstract
It has been determined by studies that the Hibiscus sabdariffa L. Plant has many biological activities such as antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant, anticarcinogenic, anticholesterol, antidiabetic and antihypertensive. This experiment tested whether Hibiscus sabdariffa L. plant extract could protect S. cerevisiae from oxidative damage caused by carbon tetrachloride (CCl4). In this study, four groups were formed. Groups: (i) Control Group: Group planted only with yeast; (ii) Hibiscus Group: Hibiscus-provided group (10%); (iii) CCl4 Group: CCl4 given group (15 millimeters); (iv) Hibiscus +CCl4, granted group: Hibiscus given group (10%). S. cerevisiae cultures were grown at 30 degrees Celsius for one, three, five, and twenty-four hours. A spectrophotometer was used to measure the time of cell development, lipid peroxidation, malondialdehyde (MDA) analysis, catalase activity determination (CAT), and glutathione (GSH) levels. The total protein changes in S. cerevisiae cultures at 1, 3, 5, and 24 hours were identified using SDS-PAGE electrophoresis and calculated using the Bradford method. Therefore, the purpose of this experiment was to determine the therapeutic action of plant extract from the polyphenol-rich species Hibiscus sabdariffa L. and to uncover the plant's potential for use as a medication to treat a variety of ailments. Our study is unusual since there hasn't been enough research done on Hibiscus sabdariffa L. plant extract in the literature that is currently available in our nation. It has special significance, particularly when it comes to examining the role and promoting mechanism of plant extract Hibiscus sabdariffa L. in protein synthesis, which is referred to as the genesis of life. Because of its potent bioactive qualities, Hibiscus sabdariffa L. plant extract is believed to assist in eradicating a wide range of health issues when utilized on a daily basis. Issues that exist in society, it will favorably contribute to the improvement of the nation's medical expenses.
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